Evaluation of a 14.5 kDa-Fasciola gigantica fatty acid binding protein as a diagnostic antigen for human fascioliasis

2011 ◽  
Vol 110 (5) ◽  
pp. 1863-1871 ◽  
Author(s):  
Gamal Allam ◽  
Ibrahim R. Bauomy ◽  
Zeinab M. Hemyeda ◽  
Thabet F. Sakran
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fasciola Gigantica ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Diagnostic Antigen ◽  
Human Fascioliasis

Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

2011 ◽  
Vol 110 (1) ◽  
pp. 419-426 ◽  
Author(s):  
Niranjan Kumar ◽  
Varghese Anju ◽  
Nagar Gaurav ◽  
Dinesh Chandra ◽  
S. Samanta ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fasciola Gigantica ◽  
Glutathione S Transferase ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Diagnostic potential ofFasciola gigantica-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis

2012 ◽  
Vol 87 (2) ◽  
pp. 147-153 ◽  
Author(s):  
G. Allam ◽  
I.R. Bauomy ◽  
Z.M. Hemyeda ◽  
T.M. Diab ◽  
T.F. Sakran
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Sandwich Elisa ◽  
Fasciola Gigantica ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Efficacy ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Diagnostic Potential

AbstractThe 14.5 kDa fatty acid binding protein (FABP) was isolated from the crude extract of adultFasciola giganticaworms. Polyclonal anti-FABP IgG was generated in rabbits immunized with prepared FABP antigen. Sandwich enzyme-linked immunosorbent assay (ELISA) was applied to detect coproantigen in stools and circulatingFasciolaantigen (CA) in sera of 126 water buffaloes by using purified and horseradish peroxidase (HRP)-conjugated anti-FABP IgG. Sandwich ELISA sensitivity was 96.97% and 94.95%; while specificity was 94.12% and 82.35% for coproantigen and CA detection, respectively. However, sensitivity and specificity of the Kato–Katz technique was 73.74% and 100%, respectively. The diagnostic efficacy of sandwich ELISA was 96.55% and 93.1% for coproantigen and CA detection, respectively. In contrast, the diagnostic efficacy of the Kato–Katz technique was 77.59%. In conclusion, these results demonstrate that the purified 14.5 kDa FABP provides a more suitable antigen for immunodiagnosis of early and current bubaline fascioliasis by using sandwich ELISA.

Vaccination of buffaloes with Fasciola gigantica recombinant fatty acid binding protein

2005 ◽  
Vol 97 (2) ◽  
pp. 129-135 ◽  
Author(s):  
P. Azhahia Nambi ◽  
S. C. Yadav ◽  
O. K. Raina ◽  
D. Sriveny ◽  
Mohini Saini
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fasciola Gigantica ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Calcium-dependent release of adipocyte fatty acid binding protein from human adipocytes

2014 ◽  
Vol 122 (03) ◽  
Author(s):  
I Schlottmann ◽  
M Ehrhart-Bornstein ◽  
M Wabitsch ◽  
SR Bornstein ◽  
V Lamounier-Zepter
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Human Adipocytes ◽  
Fatty Acid Binding ◽  
Calcium Dependent ◽  
Acid Binding Protein
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