The role of domestic tap water on Acanthamoeba keratitis in non-contact lens wearers and validation of laboratory methods

2015 ◽  
Vol 114 (9) ◽  
pp. 3283-3289 ◽  
Author(s):  
Ismail Soner Koltas ◽  
Fadime Eroglu ◽  
Elif Erdem ◽  
Meltem Yagmur ◽  
Ferdi Tanır
Keyword(s):  
Contact Lens ◽  
Tap Water ◽  
Acanthamoeba Keratitis ◽  
Laboratory Methods

scholarly journals Acanthamoeba keratitis related to contact lens in a tertiary hospital in China

2019 ◽  
Author(s):  
Weiwei Li ◽  
Zhiqun Wang ◽  
Jinghao Qu ◽  
Yang Zhang ◽  
Xuguang Sun
Keyword(s):  
Confocal Microscopy ◽  
Contact Lens ◽  
Contact Lenses ◽  
Early Stage ◽  
Tap Water ◽  
Acanthamoeba Keratitis ◽  
Tertiary Hospital ◽  
Chi Square ◽  
Soft Contact Lenses ◽  
Chi Square Test

Abstract Backgroud To report the clinical and microbiological features of Acanthamoeba keratitis (AK) related to contact lens in a tertiary hospital in China. Methods In this retrospective study, medical results of 61 cases of AK related to contact lens were reviewed from January 2000 to December 2017. The data included patients’ demographics, lens type, history, risk factors, the disease stages, reports of corneal scrapings and culture, treatment. Moreover, the genotype identification of part of these isolates was carried out with a PCR assay and sequence analysis of the 18S ribosomal DNA gene. Results There were 64 eyes included in the study. And 32.8% of cases wore soft contact lenses, 67.2% of patients used overnight orthokeratology. In the cases (20eyes) of early stage, 65% (13 eyes) had positive results in Giemsa stained smears, trophozoites were seen in 7 eyes (35%) in 0.9% sodium chloride (NaCl) wet mounts. Six eyes (30%) were diagnosed by confocal microscopy together with clinical sign. In the orthokeratology patients, 87.8% (36/41) of cases rinsed the lenses and/or cases with tap water, while 55% of soft lens wearers wore their lens during showering. The genotype of 9 isolates was determined, all the strains belonged to genotype T4. In the orthokeratology related cases, patients required therapeutic penetrating keratoplasty after 2005 were less than before 2005 (Chi-square test, χ2= 4.209，P = 0.04). Conclusions More than two thirds of cases were associated with orthokeratology. The examinations of Giemsa stained smears, 0.9% NaCl wet mounts and confocal microscopy should be done for the highly suspected cases of early stage to help the early diagnosis. The rate of therapeutic keratoplasty was decrease after 2005 compare with before 2005 in the cases of orthokeratology.

scholarly journals Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Weiwei Li ◽  
Zhiqun Wang ◽  
Jinghao Qu ◽  
Yang Zhang ◽  
Xuguang Sun
Keyword(s):  
Confocal Microscopy ◽  
Contact Lens ◽  
Contact Lenses ◽  
Early Stage ◽  
Tap Water ◽  
Clinical Signs ◽  
Acanthamoeba Keratitis ◽  
Tertiary Hospital ◽  
Soft Contact Lenses ◽  
Number Of Patients

Abstract Background To report the clinical and microbiological features of Acanthamoeba keratitis (AK) related to contact lens use in a tertiary hospital in China. Methods In this retrospective study, the medical results of 61 cases of AK related to contact lens use from January 2000 to December 2017 were reviewed. The data included patients’ demographics, lens type, history, risk factors, disease stages, corneal scraping and culture reports, and treatments. Moreover, genotypic identification of some of the isolates was carried out with a PCR assay and sequence analysis of the 18S ribosomal DNA gene. Results There were 64 eyes included in the study. A total of 32.8% of the patients wore soft contact lenses, and 67.2% of patients used overnight orthokeratology. In the cases (20 eyes) in the early stage, 65% (13 eyes) had positive results according to Giemsa-stained smears, and 0.9% sodium chloride (NaCl) wet mounts revealed trophozoites in 7 eyes (35%). Six eyes (30%) were diagnosed by confocal microscopy combined with clinical signs. In the orthokeratology patients, 87.8% (36/41) rinsed their lenses and/or cases with tap water; 55% of soft-lens wearers wore their lenses while showering. The genotype of 9 isolates was determined, and all the strains belonged to genotype T4. In the orthokeratology group, the number of patients who required therapeutic penetrating keratoplasty after 2005 was less than that before 2005 (chi-square test, χ2 = 4.209, P = 0.04). Conclusions More than two-thirds of the cases were associated with orthokeratology. Examinations with Giemsa-stained smears, 0.9% NaCl wet mounts and confocal microscopy should be performed for patients who are highly suspected of having early-stage AK to help with early diagnosis. In the orthokeratology group, the rate of therapeutic keratoplasty after 2005 was less than that before 2005.

scholarly journals Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review

2021 ◽  
Vol 49 (3) ◽  
pp. 030006052110009
Author(s):  
Jinfang Wu ◽  
Huatao Xie
Keyword(s):  
Contact Lens ◽  
Vision Loss ◽  
Tap Water ◽  
Significant Risk ◽  
Acanthamoeba Keratitis ◽  
Ocular Infection ◽  
Increased Risk ◽  
Analytical Review ◽  
Relative Rarity ◽  
Common Risk Factors

Acanthamoeba keratitis (AK) is a rare but severe ocular infection with a significant risk of vision loss. Contact lens use is the main risk factor for AK. The orthokeratology (OK) lens, a specially designed contact lens, has been used worldwide as an effective method of myopia control. However, the OK lens is associated with an increased risk of Acanthamoeba infection. Many primary practitioners are concerned about this infection because of its relative rarity, the lack of promising therapeutic medications, and the need for referral. We herein report two cases of AK associated with OK lenses, present a systematic review of such cases, and discuss the possible reasons for the higher incidence rate of this infection in patients who wear OK lenses. We combined the clinical knowledge and skills of corneal specialists and lens experts with the sole objective of addressing these OK lens-related AK cases. We found that the most common risk factors were rinsing the lenses or lens cases with tap water. Prompt and accurate diagnosis along with adequate amoebicidal treatment are essential to ensure desirable outcomes for OK lens wearers who develop AK. Appropriate OK lens parameters and regular checkups are also important.

Acanthamoeba Keratitis Associated With Tap Water Use During Contact Lens Cleaning

2013 ◽  
Vol 39 (2) ◽  
pp. 158-161 ◽  
Author(s):  
John E. Legarreta ◽  
Amy C. Nau ◽  
Deepinder K. Dhaliwal
Keyword(s):  
Water Use ◽  
Contact Lens ◽  
Tap Water ◽  
Acanthamoeba Keratitis

scholarly journals Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

2019 ◽  
Author(s):  
Weiwei Li ◽  
Zhiqun Wang ◽  
Jinghao Qu ◽  
Yang Zhang ◽  
Xuguang Sun
Keyword(s):  
Confocal Microscopy ◽  
Contact Lens ◽  
Contact Lenses ◽  
Early Stage ◽  
Tap Water ◽  
Clinical Signs ◽  
Acanthamoeba Keratitis ◽  
Tertiary Hospital ◽  
Soft Contact Lenses ◽  
Number Of Patients

Abstract Background: To report the clinical and microbiological features of Acanthamoeba keratitis (AK) related to contact lens use in a tertiary hospital in China. Methods: In this retrospective study, the medical results of 61 cases of AK related to contact lens use from January 2000 to December 2017 were reviewed. The data included patients’ demographics, lens type, history, risk factors, disease stages, corneal scraping and culture reports, and treatments. Moreover, genotypic identification of some of the isolates was carried out with a PCR assay and sequence analysis of the 18S ribosomal DNA gene. Results: There were 64 eyes included in the study. A total of 32.8% of the patients wore soft contact lenses, and 67.2% of patients used overnight orthokeratology. In the cases (20 eyes) in the early stage, 65% (13 eyes) had positive results according to Giemsa-stained smears, and 0.9% sodium chloride (NaCl) wet mounts revealed trophozoites in 7 eyes (35%). Six eyes (30%) were diagnosed by confocal microscopy combined with clinical signs. In the orthokeratology patients, 87.8% (36/41) rinsed their lenses and/or cases with tap water; 55% of soft-lens wearers wore their lenses while showering. The genotype of 9 isolates was determined, and all the strains belonged to genotype T4. In the orthokeratology group, the number of patients who required therapeutic penetrating keratoplasty after 2005 was less than that before 2005 (chi-square test, χ 2 = 4.209, P = 0.04). Conclusions: More than two-thirds of the cases were associated with orthokeratology. Examinations with Giemsa-stained smears, 0.9% NaCl wet mounts and confocal microscopy should be performed for patients who are highly suspected of having early-stage AK to help with early diagnosis. In the orthokeratology group, the rate of therapeutic keratoplasty after 2005 was less than that before 2005.

scholarly journals Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

2019 ◽  
Author(s):  
Weiwei Li ◽  
Zhiqun Wang ◽  
Jinghao Qu ◽  
Yang Zhang ◽  
Xuguang Sun
Keyword(s):  
Confocal Microscopy ◽  
Contact Lens ◽  
Contact Lenses ◽  
Early Stage ◽  
Tap Water ◽  
Clinical Signs ◽  
Acanthamoeba Keratitis ◽  
Tertiary Hospital ◽  
Soft Contact Lenses ◽  
Number Of Patients

Abstract Background: To report the clinical and microbiological features of Acanthamoeba keratitis (AK) related to contact lens use in a tertiary hospital in China. Methods: In this retrospective study, the medical results of 61 cases of AK related to contact lens use from January 2000 to December 2017 were reviewed. The data included patients’ demographics, lens type, history, risk factors, disease stages, corneal scraping and culture reports, and treatments. Moreover, genotypic identification of some of the isolates was carried out with a PCR assay and sequence analysis of the 18S ribosomal DNA gene. Results: There were 64 eyes included in the study. A total of 32.8% of the patients wore soft contact lenses, and 67.2% of patients used overnight orthokeratology. In the cases (20 eyes) in the early stage, 65% (13 eyes) had positive results according to Giemsa-stained smears, and 0.9% sodium chloride (NaCl) wet mounts revealed trophozoites in 7 eyes (35%). Six eyes (30%) were diagnosed by confocal microscopy combined with clinical signs. In the orthokeratology patients, 87.8% (36/41) rinsed their lenses and/or cases with tap water; 55% of soft-lens wearers wore their lenses while showering. The genotype of 9 isolates was determined, and all the strains belonged to genotype T4. In the orthokeratology group, the number of patients who required therapeutic penetrating keratoplasty after 2005 was less than that before 2005 (chi-square test, χ 2 = 4.209, P = 0.04). Conclusions: More than two-thirds of the cases were associated with orthokeratology. Examinations with Giemsa-stained smears, 0.9% NaCl wet mounts and confocal microscopy should be performed for patients who are highly suspected of having early-stage AK to help with early diagnosis. In the orthokeratology group, the rate of therapeutic keratoplasty after 2005 was less than that before 2005.

The role of companionship for support and relationship satisfaction: Evidence using diary and laboratory methods

2011 ◽  
Author(s):  
Gertraud Stadler ◽  
Niall Bolger ◽  
Kenzie A. Snyder ◽  
Elizabeth A. Pitula ◽  
Patrick E. Shrout
Keyword(s):  
Relationship Satisfaction ◽  
Laboratory Methods
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