A novel fission yeast gene, kms1 +, is required for the formation of meiotic prophase-specific nuclear architecture

1997 ◽  
Vol 254 (3) ◽  
pp. 238-249 ◽  
Author(s):  
M. Shimanuki ◽  
F. Miki ◽  
D.-Q. Ding ◽  
Y. Chikashige ◽  
Y. Hiraoka ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Meiotic Prophase ◽  
Nuclear Architecture ◽  
Yeast Gene

scholarly journals Fission yeast gene structure and recognition

1994 ◽  
Vol 22 (9) ◽  
pp. 1750-1759 ◽  
Author(s):  
M.Q. Zhang ◽  
T.G. Marr
Keyword(s):  
Fission Yeast ◽  
Gene Structure ◽  
Yeast Gene

scholarly journals Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast

2006 ◽  
Vol 173 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Takamune T. Saito ◽  
Daisuke Okuzaki ◽  
Hiroshi Nojima
Keyword(s):  
Fission Yeast ◽  
Meiotic Prophase ◽  
Coiled Coil ◽  
Cell Cortex ◽  
Ph Domain ◽  
Nuclear Movement ◽  
Recombination Rates ◽  
Homologous Chromosome Pairing ◽  
Cortical Localization ◽  
Pulling Force

During meiotic prophase I of the fission yeast Schizosaccharomyces pombe, oscillatory nuclear movement occurs. This promotes homologous chromosome pairing and recombination and involves cortical dynein, which plays a pivotal role by generating a pulling force with the help of an unknown dynein anchor. We show that Mcp5, the homologue of the budding yeast dynein anchor Num1, may be this putative dynein anchor. mcp5+ is predominantly expressed during meiotic prophase, and GFP-Mcp5 localizes at the cell cortex. Moreover, the mcp5Δ strain lacks the oscillatory nuclear movement. Accordingly, homologous pairing and recombination rates of the mcp5Δ strain are significantly reduced. Furthermore, the cortical localization of dynein heavy chain 1 appears to be reduced in mcp5Δ cells. Finally, the full function of Mcp5 requires its coiled-coil and pleckstrin homology (PH) domains. Our results suggest that Mcp5 localizes at the cell cortex through its PH domain and functions as a dynein anchor, thereby facilitating nuclear oscillation.

scholarly journals Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly

2015 ◽  
Vol 211 (2) ◽  
pp. 295-308 ◽  
Author(s):  
Hui-Ju Yang ◽  
Haruhiko Asakawa ◽  
Tokuko Haraguchi ◽  
Yasushi Hiraoka
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Meiotic Prophase ◽  
Spindle Assembly Checkpoint ◽  
Meiotic Spindle ◽  
Spindle Attachment ◽  
Kinetochore Assembly ◽  
Sister Chromatids ◽  
Monopolar Spindle ◽  
Meiosis I

During meiosis, the kinetochore undergoes substantial reorganization to establish monopolar spindle attachment. In the fission yeast Schizosaccharomyces pombe, the KNL1–Spc7-Mis12-Nuf2 (KMN) complex, which constitutes the outer kinetochore, is disassembled during meiotic prophase and is reassembled before meiosis I. Here, we show that the nucleoporin Nup132 is required for timely assembly of the KMN proteins: In the absence of Nup132, Mis12 and Spc7 are precociously assembled at the centromeres during meiotic prophase. In contrast, Nuf2 shows timely dissociation and reappearance at the meiotic centromeres. We further demonstrate that depletion of Nup132 activates the spindle assembly checkpoint in meiosis I, possibly because of the increased incidence of erroneous spindle attachment at sister chromatids. These results suggest that precocious assembly of the kinetochores leads to the meiosis I defects observed in the nup132-disrupted mutant. Thus, we propose that Nup132 plays an important role in establishing monopolar spindle attachment at meiosis I through outer kinetochore reorganization at meiotic prophase.

scholarly journals Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA.

1992 ◽  
Vol 11 (13) ◽  
pp. 5111-5120 ◽  
Author(s):  
N.H. Waseem ◽  
K. Labib ◽  
P. Nurse ◽  
D.P. Lane
Keyword(s):  
Fission Yeast ◽  
Accessory Protein ◽  
Yeast Gene ◽  
Gene Encoding

Isolation and characterization of the fission yeast gene rpa42 +, which encodes a subunit shared by RNA polymerases I and III

1999 ◽  
Vol 262 (4-5) ◽  
pp. 749-757 ◽  
Author(s):  
Y. Imazawa ◽  
K. Imai ◽  
A. Fukushima ◽  
K. Hisatake ◽  
M. Muramatsu ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Rna Polymerases ◽  
Yeast Gene ◽  
Isolation And Characterization ◽  
A Subunit

Cloning and Characterization of psu1+, a New Essential Fission Yeast Gene Involved in Cell Wall Synthesis

1999 ◽  
Vol 262 (2) ◽  
pp. 368-374 ◽  
Author(s):  
Kazuo Omi ◽  
Hikaru Sonoda ◽  
Kiyoshi Nagata ◽  
Kenji Sugita
Keyword(s):  
Cell Wall ◽  
Fission Yeast ◽  
Cell Wall Synthesis ◽  
Yeast Gene

A Fission Yeast Gene (prrl+) That Encodes a Response Regulator Implicated in Oxidative Stress Response

1999 ◽  
Vol 125 (6) ◽  
pp. 1061-1066 ◽  
Author(s):  
R. Ohmiya ◽  
C. Kato ◽  
H. Yamada ◽  
H. Aiba ◽  
T. Mizuno
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Fission Yeast ◽  
Response Regulator ◽  
Oxidative Stress Response ◽  
Yeast Gene

scholarly journals A Cytoplasmic Dynein Heavy Chain Is Required for Oscillatory Nuclear Movement of Meiotic Prophase and Efficient Meiotic Recombination in Fission Yeast

1999 ◽  
Vol 145 (6) ◽  
pp. 1233-1250 ◽  
Author(s):  
Ayumu Yamamoto ◽  
Robert R. West ◽  
J. Richard McIntosh ◽  
Yasushi Hiraoka
Keyword(s):  
Fission Yeast ◽  
Heavy Chain ◽  
Meiotic Recombination ◽  
Meiotic Prophase ◽  
Fluorescent Protein ◽  
Cytoplasmic Dynein ◽  
Spindle Pole ◽  
Nuclear Movement ◽  
Homologous Chromosomes ◽  
Dynein Heavy Chain

Meiotic recombination requires pairing of homologous chromosomes, the mechanisms of which remain largely unknown. When pairing occurs during meiotic prophase in fission yeast, the nucleus oscillates between the cell poles driven by astral microtubules. During these oscillations, the telomeres are clustered at the spindle pole body (SPB), located at the leading edge of the moving nucleus and the rest of each chromosome dangles behind. Here, we show that the oscillatory nuclear movement of meiotic prophase is dependent on cytoplasmic dynein. We have cloned the gene encoding a cytoplasmic dynein heavy chain of fission yeast. Most of the cells disrupted for the gene show no gross defect during mitosis and complete meiosis to form four viable spores, but they lack the nuclear movements of meiotic prophase. Thus, the dynein heavy chain is required for these oscillatory movements. Consistent with its essential role in such nuclear movement, dynein heavy chain tagged with green fluorescent protein (GFP) is localized at astral microtubules and the SPB during the movements. In dynein-disrupted cells, meiotic recombination is significantly reduced, indicating that the dynein function is also required for efficient meiotic recombination. In accordance with the reduced recombination, which leads to reduced crossing over, chromosome missegregation is increased in the mutant. Moreover, both the formation of a single cluster of centromeres and the colocalization of homologous regions on a pair of homologous chromosomes are significantly inhibited in the mutant. These results strongly suggest that the dynein-driven nuclear movements of meiotic prophase are necessary for efficient pairing of homologous chromosomes in fission yeast, which in turn promotes efficient meiotic recombination.

Sequence of a fission yeast gene encoding a protein with extensive homology to eukaryotic elongation factor-1 γ

Gene ◽  
1993 ◽  
Vol 134 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Momoi Hiroyuki ◽  
Yamada Hisami ◽  
Ueguchi Chiharu ◽  
Mizuno Takeshi
Keyword(s):  
Fission Yeast ◽  
Elongation Factor ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Eukaryotic Elongation Factor ◽  
Elongation Factor 1
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