α and β subunits of F1-ATPase are required for survival of petite mutants in Saccharomyces cerevisiae

1999 ◽  
Vol 262 (4-5) ◽  
pp. 898-908 ◽  
Author(s):  
X. J. Chen ◽  
G. D. Clark-Walker
Keyword(s):  
Saccharomyces Cerevisiae ◽  
F1 Atpase ◽  
Petite Mutants ◽  
Α And Β Subunits

Production of petite mutants of Saccharomyces cerevisiae by patulin

1969 ◽  
Vol 17 (3) ◽  
pp. 454-456 ◽  
Author(s):  
Vernon W. Mayer ◽  
Marvin S. Legator
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Petite Mutants

News & Notes: Correlation of Resistance to the Alkaloid Lycorine with the Degree of Suppressiveness in Petite Mutants of Saccharomyces cerevisiae

1997 ◽  
Vol 34 (6) ◽  
pp. 382-384 ◽  
Author(s):  
Angelica Del Giudice ◽  
Domenica R. Massardo ◽  
Filomena Manna ◽  
Natalia Koltovaya ◽  
Hans Hartings ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Petite Mutants

scholarly journals Expression of Bovine F1-ATPase with Functional Complementation in Yeast Saccharomyces cerevisiae

2005 ◽  
Vol 280 (23) ◽  
pp. 22418-22424 ◽  
Author(s):  
Neeti Puri ◽  
Jie Lai-Zhang ◽  
Scott Meier ◽  
David M. Mueller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Mass ◽  
Atp Synthase ◽  
Specific Activity ◽  
Functional Complementation ◽  
Yeast Saccharomyces Cerevisiae ◽  
F1 Atpase ◽  
Mitochondrial Atp Synthase ◽  
Genes Encoding ◽  
Molecular Machinery

The mitochondrial F1F0-ATP synthase is a multimeric enzyme complex composed of at least 16 unique peptides with an overall molecular mass of ∼600 kDa. F1-ATPase is composed of α3β3γδϵ with an overall molecular mass of 370 kDa. The genes encoding bovine F1-ATPase have been expressed in a quintuple yeast Saccharomyces cerevisiae deletion mutant (ΔαΔβΔγΔδΔϵ). This strain expressing bovine F1 is unable to grow on medium containing a non-fermentable carbon source (YPG), indicating that the enzyme is non-functional. However, daughter strains were easily selected for growth on YPG medium and these were evolved for improved growth on YPG medium. The evolution of the strains was presumably due to mutations, but mutations in the genes encoding the subunits of the bovine F1-ATPase were not required for the ability of the cell to grow on YPG medium. The bovine enzyme expressed in yeast was partially purified to a specific activity of about half of that of the enzyme purified from bovine heart mitochondria. These results indicate that the molecular machinery required for the assembly of the mitochondrial ATP synthase is conserved from bovine and yeast and suggest that yeast may be useful for the expression, mutagenesis, and analysis of the mammalian F1- or F1F0-ATP synthase.

The induction of cytoplasmic petite mutants of Saccharomyces cerevisiae by hydrostatic pressure

1977 ◽  
Vol 26 (1) ◽  
pp. 373-385
Author(s):  
M.P. Rosin ◽  
A.M. Zimmerman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hydrostatic Pressure ◽  
Cell Survival ◽  
Growth Stage ◽  
Lag Phase ◽  
Pressure Treatment ◽  
Tetrad Analysis ◽  
Petite Mutants ◽  
Wide Range ◽  
Inductive Agent

This study demonstrates that hydrostatic pressure is a potent inductive agent of the petite mutation in cultures of Saccharomyces cerevisiae. The inductive capacity of this mutagen is dependent on the magnitude and the duration of the pressure treatment. Furthermore, the extent of petite induction varies with the growth stage of the culture. Induction occurs in pressure-treated (1-4 X 1-(4) lbf in.-2 or 9–66 X 10(4) kN m-2 for 4 h) log growth cultures but not in stationary or lag phase cultures. Petite induction and cell survival are also dependent on the particular strain of yeast which is pressure-treated. Tetrad analysis and complementation assays demonstrate that pressure-induced petite cells are cytoplasmic in nature. Moreover, induced petite cells show a wide range of suppressivity (2–99%) with a large proportion of the petite cells being highly suppressive.

Single Site Catalysis of the F1-ATPase from Saccharomyces cerevisiae and the Effect of Inorganic Phosphate on It1

1987 ◽  
Vol 102 (2) ◽  
pp. 273-279 ◽  
Author(s):  
Jin KONISHI ◽  
Masafumi YOHDA ◽  
Tadao HASHIMOTO ◽  
Masasuke YOSHIDA
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inorganic Phosphate ◽  
Single Site ◽  
F1 Atpase
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