Distribution of phosphorylated protein kinase C alpha in goldfish retinal bipolar synaptic terminals: control by state of adaptation and pharmacological treatment

2006 ◽  
Vol 327 (2) ◽  
pp. 209-220 ◽  
Author(s):  
Uwe D. Behrens ◽  
Johannes Borde ◽  
Andreas F. Mack ◽  
Hans-Joachim Wagner
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Pharmacological Treatment ◽  
Phosphorylated Protein ◽  
Synaptic Terminals ◽  
Kinase C ◽  
Protein Kinase C Alpha

scholarly journals Studies on the phosphorylation of protein kinase C-α

1992 ◽  
Vol 283 (2) ◽  
pp. 515-518 ◽  
Author(s):  
C Pears ◽  
S Stabel ◽  
S Cazaubon ◽  
P J Parker
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Alpha Activity ◽  
Phosphate Content ◽  
Phosphorylated Protein ◽  
Kinase C ◽  
Protein Kinase C Alpha ◽  
The Relationship

A kinase-defective protein kinase C-alpha mutant is shown to be a phosphoprotein when expressed in COS-1 cells, indicating that intramolecular phosphorylation does not fully account for the phosphate content of protein kinase C-alpha. Furthermore, evidence is presented that the intermolecular phosphorylation of protein kinase C-alpha is due to an activity other than protein kinase C-alpha itself, and this phosphorylation appears to be necessary for protein kinase C-alpha activity. By contrast, the characteristic shift in apparent molecular mass consequent on phosphorylation in vivo can be accounted for by autophosphorylation, as demonstrated in vitro. The relationship between these phosphorylated protein kinase C-alpha species is discussed.

scholarly journals Association of protein kinase-C-alpha with cytoplasmic vesicles in melanoma cells.

1996 ◽  
Vol 44 (2) ◽  
pp. 177-182 ◽  
Author(s):  
J Timar ◽  
B Liu ◽  
R Bazaz ◽  
K V Honn
Keyword(s):  
Plasma Membrane ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Fluid Phase ◽  
Subcellular Fractionation ◽  
Melanoma Cells ◽  
Kinase C ◽  
Cytoplasmic Vesicles ◽  
Protein Kinase C Alpha ◽  
Pkc Alpha

In B16a melanoma cells, protein kinase-C-alpha (PKC alpha) is immunomorphologically associated with cytoplasmic vesicles in addition to the previously observed locations (plasma membrane, cytoskeleton, nucleus), as detected with monoclonal antibody (MAb) MC3a. Subcellular fractionation indicated that the authentic 80-KD protein as well as PKC activity can be detected in several particulate fractions except for L2, which contains dense lysosomes. The highest PKC activity is associated with the cytosol-ultralight vesicles and the L1 fraction (containing plasma membrane, endosomes, and the Golgi apparatus). Both of these fractions contained the fluid-phase endocytosis marker peroxidase, indicating that PKC alpha, in addition to other subcellular structures, is most probably associated with endosomal membranes in B16a melanoma cells.

Protein Kinase C Alpha and Tumorigenesis of the Endocrine Gland

1997 ◽  
Vol 47 (4-6) ◽  
pp. 140-144 ◽  
Author(s):  
Corinne Prévostel ◽  
Annick Martin ◽  
Véronique Alvaro ◽  
Claude Jaffiol ◽  
Dominique Joubert
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Endocrine Gland ◽  
Kinase C ◽  
Protein Kinase C Alpha
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