Turkish Validity and Reliability of the Individual Cultural Values Scale on Nurses

Background Nurses need to understand their own cultures in order to care for patients in ways that are based on the cultural structure of the patient, which means to the patient’s cultural values and beliefs. Aim This study was conducted in Turkey, and the aim of the study was to test the Turkish validity and reliability of the Individual Cultural Values Scale (CVSCALE) for nurses. Methods This research was a methodological study. The sample of the research was composed of 256 nurses. The reliability and validity analyses were performed such as additivity, Cronbach’s alpha, response bias, language, content, and construct validity. Ethical approval was obtained for the research. Results The content validity index of the scale was .91. As a result of confirmatory factor analysis, it was determined that the model had a good fit, and five dimensions of the scale were confirmed. The internal consistencies of subscales, except those for power distance and masculinity, were very reliable. The test–retest correlations were found to be very high for the CVSCALE. Conclusion The Turkish form of the Individual Cultural Values Scale that was conducted on nurses had acceptable levels of validity and reliability. Measuring culture at the individual level is important for transcultural nursing, and it will contribute the creation of nursing policies in Turkey.

Occipital neural dynamics in cannabis and alcohol use: independent effects of addiction

Alcohol and cannabis use disorder (AUD/CUD) are two of the most common addictive disorders. While studies are beginning to understand the neural changes related to acute and chronic use, few studies have examined the independent effects of AUD and CUD on neural oscillatory activity. We examined 45 adults who reported current use of both cannabis and alcohol. Participants underwent the SCID-V to determine whether they met criteria for AUD and/or CUD. Participants also completed a visual-spatial processing task while undergoing magnetoencephalography (MEG). ANCOVA with a 2 × 2 design was then used to identify the main effects of AUD and CUD on source-level oscillatory activity. Of the 45 adults, 17 met criteria for AUD, and 26 met criteria for CUD. All participants, including comparison groups, reported use of both cannabis and alcohol. Statistical analyses showed a main effect of AUD, such that participants with AUD displayed a blunted occipital alpha (8–16 Hz) response. Post-hoc testing showed this decreased alpha response was related to increased AUD symptoms, above and beyond amount of use. No effects of AUD or CUD were identified in visual theta or gamma activity. In conclusion, AUD was associated with reduced alpha responses and scaled with increasing severity, independent of CUD. These findings indicate that alpha oscillatory activity may play an integral part in networks affected by alcohol addiction.

The Right Lateral Prefrontal Cortex Impacts Control Perception as a Function of Probabilistic Stimulus Processing

Being able to control inner and environmental states is a basic need of living creatures. Control perception (CP) itself may be neurally computed as the subjective ratio of outcome probabilities given the presence and the absence of behavior. If behavior increases the perceived probability of a given outcome, action-outcome contingency is met, and CP may emerge. Nonetheless, in regard of this model, not much is known on how the brain processes CP from these information. This study uses low-intensity transcranial focused ultrasound neuromodulation in a randomized-controlled doubleblind cross-over design to investigate the impact of the right inferior frontal gyrus on this process. Fourty healthy participants visited the laboratory twice (once in a sham, once in a neuromodulation condition) and rated their control perception regarding a classical control illusion task. EEG alpha and theta power density were analyzed in a hierarchical single trial based mixed modeling approach. Results indicate that the right lateral PFC modulates action-outcome learning by providing stochastic information about the situation with increased alpha responses during low control situations (in which the ratio of probabilities is zero). Furthermore, this alpha response was found to modulate mid-frontal theta by altering its relationship with self-reported effort and worrying. These data provide evidencefor right lateral PFC mediated probabilistic stimulus processing during the emergence of CP.

PD-L1 Dependent Immunogenic Landscape in Hot Lung Adenocarcinomas Identified by Transcriptome Analysis

Background: Lung cancer is the most frequent cause of cancer-related deaths worldwide. The clinical development of immune checkpoint blockade has dramatically changed the treatment paradigm for patients with lung cancer. Yet, an improved understanding of PD-1/PD-L1 checkpoint blockade-responsive biology is warranted. Methods: We aimed to identify the landscape of immune cell infiltration in primary lung adenocarcinoma (LUAD) in the context of tumoral PD-L1 expression and the extent of immune infiltration (“hot” vs. “cold” phenotype). The study comprises LUAD cases (n = 138) with “hot” (≥150 lymphocytes/HPF) and “cold” (<150 lymphocytes/HPF) tumor immune phenotype and positive (>50%) and negative (<1%) tumor PD-L1 expression, respectively. Tumor samples were immunohistochemically analyzed for expression of PD-L1, CD4, and CD8, and further investigated by transcriptome analysis. Results: Gene set enrichment analysis defined complement, IL-JAK-STAT signaling, KRAS signaling, inflammatory response, TNF-alpha signaling, interferon-gamma response, interferon-alpha response, and allograft rejection as significantly upregulated pathways in the PD-L1-positive hot subgroup. Additionally, we demonstrated that STAT1 is upregulated in the PD-L1-positive hot subgroup and KIT in the PD-L1-negative hot subgroup. Conclusion: The presented study illustrates novel aspects of PD-L1 regulation, with potential biological relevance, as well as relevance for immunotherapy response stratification.

Foetal Immune Response Activation and High Replication Rate during Generation of Classical Swine Fever Congenital Infection

Classical swine fever virus (CSFV) induces trans-placental transmission and congenital viral persistence; however, the available information is not updated. Three groups of sows were infected at mid-gestation with either a high, moderate or low virulence CSFV strains. Foetuses from sows infected with high or low virulence strain were obtained before delivery and piglets from sows infected with the moderate virulence strain were studied for 32 days after birth. The low virulence strain generated lower CSFV RNA load and the lowest proportion of trans-placental transmission. Severe lesions and mummifications were observed in foetuses infected with the high virulence strain. Sows infected with the moderately virulence strain showed stillbirths and mummifications, one of them delivered live piglets, all CSFV persistently infected. Efficient trans-placental transmission was detected in sows infected with the high and moderate virulence strain. The trans-placental transmission occurred before the onset of antibody response, which started at 14 days after infection in these sows and was influenced by replication efficacy of the infecting strain. Fast and solid immunity after sow vaccination is required for prevention of congenital viral persistence. An increase in the CD8+ T-cell subset and IFN-alpha response was found in viremic foetuses, or in those that showed higher viral replication in tissue, showing the CSFV recognition capacity by the foetal immune system after trans-placental infection.

Protein profiling and network enrichment analysis in individuals before and after the onset of rheumatoid arthritis

Background Antibodies and upregulated cytokines and chemokines predate the onset of rheumatoid arthritis (RA) symptoms. We aimed to identify the pathways related to the early processes leading to RA development, as well as potential novel biomarkers, using multiple protein analyses. Methods A case-control study was conducted within the Biobank of northern Sweden. The plasma samples from 118 pre-symptomatic individuals (207 samples; median predating time 4.1 years), 79 early RA patients, and 74 matched controls were analyzed. The levels of 122 unique proteins with an acknowledged relationship to autoimmunity were analyzed using 153 antibodies and a bead-based multiplex system (FlexMap3D; Luminex Corp.). The data were analyzed using multifactorial linear regression model, random forest, and network enrichment analysis (NEA) based on the 10 most significantly differentially expressed proteins for each two-by-two group comparison, using the MSigDB collection of hallmarks. Results There was a high agreement between the different statistical methods to identify the most significant proteins. The adipogenesis and interferon alpha response hallmarks differentiated pre-symptomatic individuals from controls. These two hallmarks included proteins involved in innate immunity. Between pre-symptomatic individuals and RA patients, three hallmarks were identified as follows: apical junction, epithelial mesenchymal transition, and TGF-β signaling, including proteins suggestive of cell interaction, remodulation, and fibrosis. The adipogenesis and heme metabolism hallmarks differentiated RA patients from controls. Conclusions We confirm the importance of interferon alpha signaling and lipids in the early phases of RA development. Network enrichment analysis provides a tool for a deeper understanding of molecules involved at different phases of the disease progression.