Enhanced gellan gum production by hydrogen peroxide (H2O2) induced oxidative stresses in Sphingomonas paucimobilis

2013 ◽  
Vol 37 (4) ◽  
pp. 743-748 ◽  
Author(s):  
Guilan Zhu ◽  
Long Sheng ◽  
Qunyi Tong
Keyword(s):  
Hydrogen Peroxide ◽  
Gellan Gum ◽  
Sphingomonas Paucimobilis ◽  
Oxidative Stresses

Gellan gum biosynthesis in Sphingomonas paucimobilis ATCC 31461: Genes, enzymes and exopolysaccharide production engineering

2002 ◽  
Vol 29 (4) ◽  
pp. 170-176
Author(s):  
I Sá-Correia ◽  
A M Fialho ◽  
P Videira ◽  
L M Moreira ◽  
A R Marques ◽  
...  
Keyword(s):  
Gellan Gum ◽  
Sphingomonas Paucimobilis ◽  
Production Engineering ◽  
Exopolysaccharide Production

Effect of 2-deoxy-d-glucose on gellan gum biosynthesis by Sphingomonas paucimobilis

2019 ◽  
Vol 42 (5) ◽  
pp. 897-900 ◽  
Author(s):  
Guilan Zhu ◽  
Na Guo ◽  
Yanan Yong ◽  
Yawen Xiong ◽  
Qunyi Tong
Keyword(s):  
Gellan Gum ◽  
Sphingomonas Paucimobilis

scholarly journals Characterization, Antimicrobial Properties and Coatings Application of Gellan Gum Oxidized with Hydrogen Peroxide

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 31 ◽  
Author(s):  
Yushuang Lu ◽  
Xiaojian Zhao ◽  
Sheng Fang
Keyword(s):  
Hydrogen Peroxide ◽  
1H Nmr Spectroscopy ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Gellan Gum ◽  
Proton Nuclear Magnetic Resonance ◽  
Carboxyl Groups ◽  
Gram Negative Bacteria ◽  
Gram Positive Bacteria ◽  
H2o2 Oxidation

The effect of hydrogen peroxide (H2O2) oxidation on the physicochemical, gelation and antimicrobial properties of gellan gum was studied. The oxidized gellan gum (OGG) was characterized by measuring the carboxyl/carbonyl group contents, Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H-NMR) spectroscopy. The H2O2 oxidation resulted in a large increase in the carboxyl groups in gellan gum. The OGG lost gelation ability by oxidation even in the presence of metal ions. The antimicrobial activities of the OGG against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli), and fungal (Aspergillus niger) were tested. The OGG could inhibit the growth of both bacteria and fungal, and the activity was improved with an increase in the oxidation level. Finally, the application of the OGG as an active coatings material to extend the storage of apples was tested.

Optimization of culture medium compositions for gellan gum production by a halobacterium Sphingomonas paucimobilis

2015 ◽  
Vol 115 ◽  
pp. 694-700 ◽  
Author(s):  
Jun Zhang ◽  
Ya-chen Dong ◽  
Lin-lin Fan ◽  
Zhi-hua Jiao ◽  
Qi-he Chen
Keyword(s):  
Culture Medium ◽  
Gellan Gum ◽  
Sphingomonas Paucimobilis

scholarly journals The catalase and superoxide dismutase genes are transcriptionally up-regulated upon oxidative stress in the strictly anaerobic archaeon Methanosarcina barkeri

Microbiology ◽  
2006 ◽  
Vol 152 (6) ◽  
pp. 1671-1677 ◽  
Author(s):  
Andrei L. Brioukhanov ◽  
Alexander I. Netrusov ◽  
Rik I. L. Eggen
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Adaptive Response ◽  
Methanosarcina Barkeri ◽  
Strictly Anaerobic ◽  
Dot Blot ◽  
Oxidative Stresses ◽  
Stress Agent ◽  
Dot Blot Analysis

Methanosarcina barkeri is a strictly anaerobic methanogenic archaeon, which can survive oxidative stress. The oxidative stress agent paraquat (PQ) suppressed growth of M. barkeri at concentrations of 50–200 μM. Hydrogen peroxide (H2O2) inhibited growth at concentrations of 0.4–1.6 mM. Catalase activity in cell-free extracts of M. barkeri increased about threefold during H2O2 stress (1.3 mM H2O2, 2–4 h exposure) and nearly twofold during superoxide stress (160 μM PQ, 2 h exposure). PQ (160 μM, 2–4 h exposure) and H2O2 (1.3 mM, 2 h exposure) also influenced superoxide dismutase activity in cell-free extracts of M. barkeri. Dot-blot analysis was performed on total RNA isolated from H2O2- and PQ-exposed cultures, using labelled internal DNA fragments of the sod and kat genes. It was shown that H2O2 but not PQ strongly induced up-regulation of the kat gene. PQ and to a lesser degree H2O2 induced the expression of superoxide dismutase. The results indicate the regulation of the adaptive response of M. barkeri to different oxidative stresses.

scholarly journals The mechanism of improved gellan gum production by two-stage culture of Sphingomonas paucimobilis

3 Biotech ◽  
2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Guilan Zhu ◽  
Xiaozhong Cheng ◽  
Zijing Fu ◽  
Zhilan Zhang ◽  
Qunyi Tong
Keyword(s):  
Gellan Gum ◽  
Sphingomonas Paucimobilis ◽  
Two Stage ◽  
Two Stage Culture

Effects of carbon/nitrogen ratio, dissolved oxygen and impeller type on gellan gum production in Sphingomonas paucimobilis

2011 ◽  
Vol 62 (1) ◽  
pp. 299-305 ◽  
Author(s):  
Jin Huang ◽  
Shu Jiang ◽  
Xiaoqin Xu ◽  
Haishui Wu ◽  
Xiangcheng Zhu ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Gellan Gum ◽  
Sphingomonas Paucimobilis ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio ◽  
Impeller Type
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