ENHANCEMENT OF TOMATO ROOT-KNOT NEMATODES CONTROL USING A NOVEL BIOLOGICAL TREATMENT IN NORTH SINAI

This study focuses on using olive mill waste water (OMWW) as a novel biological treatment to decrease plant diseases while improving food quality. Root-knot nematode Meloidogyne is considered one of the main tomato diseases in North Sinai. The current study aimed to evaluate the efficiency of diffracting olive mill waste waters concentrations compared with the nematicide Oxamylon on Tomato Root-knot. OMWW was applied daily to tomato plants growing in sandy loam soil for three months. Represented five different concentration of OMWW (0%, 25%, 50%, 75%, and 100%) used for irrigation during the experiment. The positive control treatment was treated with Oxamyl at recommended dose. The negative control was watered only with tap water (0%). The results showed that mortality rates of M. incognita were significantly affected by concentrations. All treatments were superior to the untreated control in reducing nematode, except for OMWWs at 100 %, which was equivalent to the control treatment. The largest drop in root galling numbers Oxamyl was achieved (75.73%) was followed by 50% at (73.81%), 75% (68.01%), and 25% (39.67%). The highest reductions rated were recorded with the 50%and 75% concentrations used of OMWW. Overall, in the effort of efficient biological treatments was achieved for root knot control measures in tomato, the OMWW application may be used as one factor of integrated root-knot nematode management.

Dietary Supplementation of Olive Mill Waste Water Polyphenols in Rabbits: Evaluation of the Potential Effects on Hepatic Apoptosis, Inflammation and Metabolism through RT-qPCR Approach

Agro-industrial processing for the production of food or non-food products generates a wide range of by-products and residues rich in bioactive compounds including polyphenols. The concentration of these by-products is sometimes higher than in the original raw material as in the case of olive mill waste water (OMWW), one of the main by-products of olive oil extraction. Polyphenols are secondary plant metabolites that regulate the expression of specific inflammatory genes, transcriptional factors and pro/anti-apoptotic molecules, thus modulating the signaling pathways essential for cell health and homeostasis. The liver plays a key role in regulating homeostasis by responding to dietary changes in order to maintain nutritional and physiological states. In this study a nutrigenomic approach was adopted, which focuses on the effects of diet–health–gene interactions and the modulation of cellular processes, in order to evaluate the expression of the genes (AGER, BAX, COX2, IL1B, PPARA, PPARG, SIRT1, TNFA) involved in these interactions in the livers of rabbits fed with a diet supplemented with OMWW (POL) or without supplements (control, CTR). The RT-qPCR analysis showed the down-regulation of SIRT1, TNFA, AGER, BAX and PPARA transcripts in the POL group compared to the CTR group. These results show that OMWW dietary supplementation prevents cell death and tissue deterioration in rabbits.

A Polyphenol-Rich Extract of Olive Mill Wastewater Enhances Cancer Chemotherapy Effects, While Mitigating Cardiac Toxicity

Cardiovascular toxicity remains one of the most adverse side effects in cancer patients receiving chemotherapy. Extra-virgin olive oil (EVOO) is rich in cancer preventive polyphenols endowed with anti-inflammatory, anti-oxidant activities which could exert protective effects on heart cells. One very interesting derivative of EVOO preparation is represented by purified extracts from olive mill waste waters, (OMWW) rich in polyphenols. Here, we have investigated the anti-cancer activity, of a OMWW preparation named A009 when combined with chemotherapeutics as well as its potential cardioprotective activities, Mice bearing prostate cancer (PCa) xenografts were treated with cisplatin, alone or in combination with A009. In an in vivo model, we found synergisms of A009 and cisplatin in reduction of prostate cancer tumor weight. Hearts of mice were analyzed, and the mitochondria were studied by transmission electron microscopy. The hearts of mice co-treated with A009 extracts along with cisplatin had reduced mitochondria damage compared to the ones treated with chemotherapy alone, indicating a cardioprotective role. To confirm the in vivo results, tumor cell lines and rat cardiomyocytes were treated with cisplatin in vitro with and without A009. Another frequently used chemotherapeutic agent 5-fluorouracil (5-FU), was also tested in this assay, observing a similar effect. A009 in vitro was additive to cisplatin and 5-FU to reduce cancer cell growth, while it did not further reduced growth of rat cardiomyocytes treated with cisplatin and 5-FU. A009 cardioprotective effects towards 5-FU chemotherapy were further investigated in vitro, using cardiomyocytes freshly isolated from mice pups. A009 mitigated toxicity of the fluoropyrimidine. Our study demonstrates that the polyphenol rich purified A009 extracts enhances the effect of chemotherapy in vitro and in vivo, but mitigates chemotherpy adverse effects on heart and on isolated cardiomyocytes. Olive mill waste water extracts could therefore represent a potential candidate for cardiovascular prevention in patients undergoing cancer chemotherapy.

Effect of storage time on the biodegradability of olive oil mill wastewater from the cold extraction of olive oil system

The olive oil processing industry’s liquid effluents (OMW) have a polluting capacity for the ecosystems. This study aimed to evaluate the physicochemical quality of OMW of two varieties of separated and combined olives cultivated in Khenchela Eastern Algeria, from the cold extraction of the olive oil extraction system. These was to determine their degree of pollution and biodegradability during one year of storage at ambient temperature to recommend the correct treatment for each storage time. Results of the measured parameters pH, EC,TSS percent, H2O percent, lipids, DM, OM, MM, VM, COT percent, NTK percent, C/N, BOD5, COD, BI, TOM, BOD5/COD show that wastewater from olive oil mills has an acid pH, and they are very loaded with organic matter evaluated in terms of COD and BOD5, quite filled with minerals. The storage of olive mill waste can reduce progressively the pollution caused by this waste. Whereas during one year, the reduction rate of COD, BOD5, TOM, BI, is respectively 29.4%, 54.8%, 39.16%, 54.2%, but C/N, BOD5/COD continue to increase as well as pH that continues to decrease during the storage. Accordingly, storing olive mill waste during a year reduces its pollution rate, so it is slowly biodegradable. When disposing of it, an adequate treatment procedure must be required to protect the environment.

Effects of Dietary Polyphenols from Olive Mill Waste Waters on Inflammatory and Apoptotic Effectors in Rabbit Ovary

The aim of this study was to evaluate the effect of dietary polyphenols on the expression of the effectors involved in inflammation and apoptosis in rabbit ovary. New Zealand White female rabbits were fed a basal control diet (CTR), or the same diet supplemented with a polyphenolic concentrate (POL, 282.4 mg/kg) obtained from olive mill waste waters. The follicle counts and the relative mRNA (RT-qPCR) and protein (immunohistochemistry) expression of the effectors involved in inflammation (cyclooxygenase-2; interleukin-1beta; tumor necrosis factor-alpha, TNFA) and apoptosis (BCL2-associated X protein, BAX), detected in the ovaries of both groups, were examined. The POL diet increased the primary and total follicles number. Cyclooxygenase-2 gene expression was higher (p < 0.05) in the POL group than in the CTR group, whereas BAX was lower (p < 0.05) in POL than CTR. Immunohistochemistry revealed the presence of all the proteins examined, with weaker (p < 0.05) COX2 and BAX signals in POL. No differences between the CTR and POL groups were observed for IL1B and TNFA gene and protein expression. These preliminary findings show that dietary polyphenols modulate inflammatory and apoptotic activities in rabbit ovary, regulating cyclooxygenase-2 and BAX expression, thus suggesting a functional involvement of these dietary compounds in mammalian reproduction.

Antifungal activity of hydroxytyrosol enriched extracts from olive mill waste against Verticillium dahliae, the cause of Verticillium wilt of olive

Verticillium wilt (caused by Verticillium dahliae Kleb.) is an important disease affecting olive (Olea europaea L.) production. Effective control of this disease relies on integrated management strategies. In vitro antifungal activity of two hydroxytyrosol (HTyr) enriched extracts (HTE1 and HTE2) obtained from olive mill waste products (OMWP) was assessed against V. dahliae. Inhibitory effects of pure HTyr as a standard, and HTE1 and HTE2 at different concentrations, were evaluated on mycelium growth and conidium germination of V. dahliae. Chemical characterization of HTE1 and HTE2 allowed identification and quantification of HTyr as the main constituent in both extracts along with other low molecular weight phenols. HTE1 showed a higher inhibitory activity in both growth and conidium germination of V. dahliae. At the tested concentrations, low antifungal effects of HTyr were observed. After 3 d, 1 mg mL-1 of HTE1 gave greater inhibition of mycelium growth than HTE2 or HTyr. After 24 h, HTE1 gave 55% inhibition of conidium germination, and HTyr and HTE2 both gave 37% inhibition. This study has demonstrated that phenolic compounds derived from OMWP have antifungal activity against V. dahliae, indicating the potential of these compounds for eco-friendly control of Verticillium wilt.