Erratum to: Influence of photoperiods on the growth rate and biomass productivity of green microalgae

Fortification of effective microbes (EM) such as Pediococcus acidilactici and photosynthetic bacteria in fertilizer and animal feed exhibiting effective outcomes in enhancing crops and livestock production as well as upholds sustainable agriculture concept. However, the productivity of the EM biomass stocks is unsurpassed to the demand due to non-optimized media. In this study, two sets of nutrient pair comprising of beef extract-manganese and yeast extract-glucose for P. acidilactici and photosynthetic bacteria respectively were studied. The optimum media formulated was based on the highest growth rate in 96-microwell plate system were subsequently tested in 1 L cultures volume for EM biomass production. Results showed that growth rate and dry weight of P. acidilactici in media supplemented with 64 g/L beef extract (0.145 h-1, 0.243 g) and 0.28 g/L manganese (0.1134 h-1, 0.32 g) were higher compared to control medium with an increment of 17.39 % and 54.59 % biomass productivity as compared to MRS medium respectively. Meanwhile, the growth rate and dry weight for Photosynthetic bacteria in media supplemented with 6.63 g/L glucose (0.2538 h-1, 0.167 g) and 0.44 g/L yeast extract (0.2733 h-1, 0.163 g) recorded 11.3 % and 8.73 % biomass productivity increment in comparison to the HCH medium.

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Effects of nitrogen limitation on uptake of inorganic carbon and specific activity of ribulose-1,5-bisphosphate carboxylase/oxygenase in green microalgae

Canadian Journal of Botany ◽

10.1139/b91-147 ◽

1991 ◽

Vol 69 (5) ◽

pp. 1146-1150 ◽

Cited By ~ 36

Author(s):

John Beardall ◽

Simon Roberts ◽

Jenny Millhouse

Keyword(s):

Growth Rate ◽

Key Words ◽

Growth Rates ◽

Nitrogen Limitation ◽

Inorganic Carbon ◽

Marked Decrease ◽

Specific Activity ◽

Green Microalgae ◽

Bisphosphate Carboxylase ◽

Cell Basis

The effects of nitrogen limitation on the characteristics of inorganic carbon-dependent O2 evolution have been examined in the green microalgae Chlorella emersonii and Gloeomonas sp. When cells were grown under 5% CO2 or air, decreasing the growth rate (increasing nitrogen limitation) caused a decrease in maximum rates of photosynthetic O2 evolution, although when expressed on a per cell basis such changes were only evident at growth rates below 0.16 day−1. Severe nitrogen limitation also caused a marked decrease in k1/2 (CO2) in light- and CO2-dependent O2 evolution. Although values for this parameter were not as low as for low CO2 grown cells with a fully induced CO2-concentrating mechanism, they were less than one-half the corresponding values from cells with the mechanism fully repressed. Nitrogen-limitation also resulted in decreases in the activity and cellular content of ribulose-1,5-bisphosphate carboxylase/oxygenase. The changes to activity and levels of this enzyme were not equivalent so the specific activity decreased dramatically between growth rates of 0.57 and 0.25 day−1. Similar effects were noted in Gloeomonas and in air-grown Chlorella. The results are discussed in relation to regulation of CO2 uptake and assimilation in microalgae. Key words: microalgae, CO2 assimilation, nitrogen limitation, Rubisco, Chlorella.

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Growth of Desmodesmus subspicatus green microalgae and nutrient removal from sugarcane vinasse clarified by electrocoagulation using aluminum or iron electrodes

DYNA ◽

10.15446/dyna.v86n211.72379 ◽

2019 ◽

Vol 86 (211) ◽

pp. 225-232

Author(s):

Mauricio Daniel Montaño Saavedra ◽

Flávia Paschino Bissoto ◽

Roniel Augusto De Souza ◽

Viktor Oswaldo Cárdenas Concha ◽

Reinaldo Gaspar Bastos

Keyword(s):

Electrode Materials ◽

Biomass Productivity ◽

Green Microalgae ◽

Chemical Production ◽

Microalgae Cultivation ◽

Iron Electrodes ◽

Desmodesmus Subspicatus ◽

High Turbidity ◽

Ph Neutralization ◽

Sugarcane Vinasse

Sugarcane ethanol production generates considerable quantities of vinasse, its main wastewater. Microalgae cultivation is a promising option for effluent remediation, since the generated biomass can be feedstock for biofuel and bio-based chemical production. Due to vinasse high turbidity, pretreatment is necessary to clarify this effluent, adapting it as a mixotrophic culture medium. In this context, the present research evaluated the integrated process of electrocoagulation (EC) of sugarcane vinasse with aluminum or iron electrodes and subsequent cultivation of green microalgae Desmodesmus subspicatus. Results indicate pH neutralization and high turbidity removal efficiency by EC with both electrode materials. Aluminum EC and subsequent microalgae cultivation removed 66 and 75% of initial total organic carbon and total nitrogen, respectively, with biomass productivity of 1.45 g L-1day-1 and maximum specific growth rate of 0.095 h-1. Microalgae productivity was inferior in vinasse pretreated by iron EC, suggesting possible interference of ferric compounds in the microalgal development.

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Production of Microalgal Slow-Release Fertilizer by Valorizing Liquid Agricultural Digestate: Growth Experiments with Tomatoes

Applied Sciences ◽

10.3390/app10113890 ◽

2020 ◽

Vol 10 (11) ◽

pp. 3890

Author(s):

Raquel Jimenez ◽

Giorgos Markou ◽

Saida Tayibi ◽

Abdellatif Barakat ◽

Camille Chapsal ◽

...

Keyword(s):

Growth Rate ◽

Slow Release ◽

Biomass Productivity ◽

Weather Conditions ◽

Added Value ◽

Batch Mode ◽

Microalgal Biomass ◽

Slow Release Fertilizer ◽

Liquid Digestate ◽

Synthetic Fertilizer

Anaerobic Digestion (AD) is a process that is well-known and fast-developing in Europe. AD generates large amounts of digestate, especially in livestock-intensive areas. Digestate has potential environmental issues due to nutrients (such as nitrogen) lixiviation or volatilization. Using liquid digestate as a nutrient source for microalgae growth is considered beneficial because digestate could be valorized and upgraded by the production of an added value product. In this work, microalgal biomass produced using liquid digestate from an agricultural biogas plant was investigated as a slow-release fertilizer in tomatoes. Monoraphidium sp. was first cultivated at different dilutions (1:20, 1:30, 1:50), in indoor laboratory-scale trials. The optimum dilution factor was determined to be 1:50, with a specific growth rate of 0.13 d−1 and a complete nitrogen removal capacity in 25 days of culture. Then, outdoor experiments were conducted in a 110 dm3 vertical, closed photobioreactors (PBRs) in batch and semi-continuous mode with 1:50 diluted liquid digestate. During the batch mode, the microalgae were able to remove almost all NH4+ and 65 (±13) % of PO43−, while the microalgal growth rate reached 0.25 d−1. After the batch mode, the cultures were switched to operate under semi-continuously conditions. The cell densities were maintained at 1.3 × 107 cells mL−1 and a biomass productivity around 38.3 mg TSS L−1 d−1 during three weeks was achieved, where after that it started to decline due to unfavorable weather conditions. Microalgae biomass was further tested as a fertilizer for tomatoes growth, enhancing by 32% plant growth in terms of dry biomass compared with the control trials (without fertilization). Similar performances were achieved in tomato growth using synthetic fertilizer or digestate. Finally, the leaching effect in soils columns without plant was tested and after 25 days, only 7% of N was leached when microalgae were used, against 50% in the case of synthetic fertilizer.

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KARAKTERISTIK PERTUMBUHAN Tetraselmis sp. DAN Nannochloropsis sp.

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.10749 ◽

2016 ◽

Vol 18 (1) ◽

pp. 15 ◽

Cited By ~ 1

Author(s):

Muhammad Fakhri ◽

Nasrullah B. Arifin

Keyword(s):

Growth Rate ◽

Optical Density ◽

Doubling Time ◽

Maximum Growth ◽

Maximum Growth Rate ◽

Growth Characteristics ◽

Green Microalgae ◽

Microalgae Growth

Monitoring of microalgae growth (Tetraselmis sp. and Nannochloropsis sp.) is one of the essential factors in fish and shrimp cultures. The purpose of this study was to determine the growth characteristics of Tetraselmis sp. and Nannochloropsis sp. by measuring optical density using spectrophotometry method. Absorbance of 600 nm was used for both species. The results showed that the maximum growth was achieved at day 6 for both microalgae with OD value of 1.734±0.013 and 1.329±0,002 for Tetraselmis sp. and Nannochloropsis sp., respectively. Tetraselmis sp. had a maximum growth rate of 0.74/day and doubling time of 22.43 hours while Nannochloropsis sp. had a maximum growth rate of 0.86/day and doubling time of 19.25 hours. This study shows that absorbance of 600 nm is suitable for determine the growth of green microalgae and spectrophotometry method can be used efficiently to monitor microalgal growth.

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Characteristics of the growth rate and lipid production in fourteen strains of Baltic green microalgae

Oceanological and Hydrobiological Studies ◽

10.1515/ohs-2018-0002 ◽

2018 ◽

Vol 47 (1) ◽

pp. 10-18 ◽

Cited By ~ 4

Author(s):

Marek Klin ◽

Filip Pniewski ◽

Adam Latała

Keyword(s):

Growth Rate ◽

Mass Culture ◽

Lipid Production ◽

Growth Patterns ◽

Green Microalgae ◽

Yield Improvement ◽

Final Cell Density ◽

Lipid Yield ◽

Green Algal ◽

High Lipid

Abstract Screening of fourteen Baltic green algal strains provided basic data on their mass culture potential for the purpose of valuable biomass production with particular emphasis on lipid content. Selected microalgae were grown under non-stressed conditions in order to identify those characterized by efficient lipid production. The tested strains exhibited significant differences in growth patterns and lipid yields. Strains belonging to Chlorella and Stichococcus genera exhibited the highest growth rates, ranging from 0.39 d-1 to 0.50 d-1 and thus the highest final cell density (> 107 cells ml-1). Furthermore, five strains: C. minutissima BA-12, C. fusca BA-18, C. vulgaris BA-80, Monoraphidium sp. BA-165 and Chlorella sp. BA-167 were characterized by distinctively high lipid yield (> 60 mg l-1). The same strains, together with C. vulgaris BA-02, were also shown as those with the highest volumetric lipid productivity, reaching > 30 mg l-1 d-1. The tested Baltic strains performed well in terms of lipid production compared to the literature data, still leaving a great spectrum of opportunities for further lipid yield improvement.

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PEMANFAATAN LIMBAH CAIR INDUSTRI TAHU UNTUK PRODUKSI BIOMASSA MIKROALGA Nannochloropsis Sp SEBAGAI BAHAN BAKU BIODIESEL

REAKTOR ◽

10.14710/reaktor.15.4.253-260 ◽

2016 ◽

Vol 15 (4) ◽

pp. 253

Author(s):

Widayat Widayat ◽

H Hadiyanto

Keyword(s):

Waste Water ◽

Growth Rate ◽

Total Lipid ◽

Liquid Waste ◽

Biomass Productivity ◽

Optimum Growth

Indonesia is one of one of productive tofu producer both for industry or home made scale. Tofu industries contribute wastewater up to 20 millions cubic meter (m3) per year. Currently, tofu waste water was treated by anaerobic and aerobic methods which reveal some disadvantages. To overcome the disadvantages of both methods, wastewater of tofu industry was used as a medium for the cultivation of microalgae. Nannochloropsis sp is one of microalgae species which has high content of lipid and thus can be converted to biodiesel. This study examined the growth rate, biomass productivity, reduced organic levels of COD and BOD, and total lipid of microalgae Nannochloropsis sp which cultivated in tofu wastewater medium. The optimum growth of Nannochloropsis sp was found at 20% vol of liquid waste water which produce lipids of 34.25 % and also can reduce COD up to 77.40 %. The optimum nutrients of NaHCO3 was showed at the composition of 70 mg / L which decrease COD up to 78.81 % and produce lipids 16.841 %. The addition of nutrition every 4 days was able to reduce the COD up to 81.835 % and produce 14.195 % yield of lipids. Keywords: tofu wastewater, Nannochloropsis sp., biodiesel, COD & BODAbstrak Indonesia merupakan salah satu negara penghasil tahu yang cukup produktif baik skala industri maupun rumahan. Industri tahu menyumbang limbah cair mencapai 20 juta meter kubik (m3) per tahun. Pada saat ini pengolahan limbah tahu cair dilakukan dengan metode anaerobik maupun aerobik. Akan tetapi kedua metode tersebut memiliki beberapa kelemahan. Untuk mengatasi kelemahan kedua metode tersebut, limbah tahu cair dapat dimanfaatkan sebagai media kultivasi mikroalga. Nannochloropsis sp merupakan mikroalga yang mampu menghasilkan lipid yang besar tersebut dapat dikonversi menjadi salah satu energi alternative biodiesel. Dalam penelitian ini dikaji laju pertumbuhan, produktivitas biomassa, penurunan kadar organik COD dan BOD, dan total lipid mikroalga jenis Nannochloropsis sp yang dikultivasi pada medium limbah tahu cair. Pertumbuhan Nannochloropsis sp. optimum pada medium 20% volume limbah tahu cair yang menghasilkan lipid sebanyak 34,25% dan dapat menurunkan COD hingga 77,40%. Penambahan nutrisi NaHCO3optimum pada komposisi 70 mg/l yang mampu menurunkan COD sebesar 78,81% dan menghasilkan lipid sebanyak 16,841%. Waktu penambahan nutrisi setiap 4 hari sekali mampu menurunkan COD sebesar 81,835% dan menghasilkan lipid sebesar 14,195%. Keywords: limbah tahu, Nannochloropsis sp., biodiesel, COD & BOD

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