Immuno-histochemical staining expression of Ki67 protein in canine mammary gland carcinoma

2010 ◽  
Vol 20 (6) ◽  
pp. 557-561 ◽  
Author(s):  
Annahita Rezaie ◽  
Abbas Tavasoli ◽  
Mitra Mehrazma ◽  
Alireza Bahonar
2009 ◽  
Vol 9 (4) ◽  
pp. 333-338 ◽  
Author(s):  
A. Rezaie ◽  
A. Tavasoli ◽  
A. Bahonar ◽  
M. Mehrazma

Author(s):  
Patricia de Faria Lainetti ◽  
Andressa Brandi ◽  
Antonio Fernando Leis Filho ◽  
Maria Carolina Mangini Prado ◽  
Priscila Emiko Kobayashi ◽  
...  

Mammary neoplasms affect a population of uncastrated and elderly female dogs and most of these neoplasms are malignant. In order to study this disease cell culture presents itself as a promising preclinical model, creating the opportunity to deposit cell lines at a cell bank, allowing a great repetition of the assays and making the validation of the results more reliable. Including, in vitro experiments for vasculogenic mimicry (VM) evaluation. VM is related to cancer cells capable of generate vascular-like structures without endothelial cells, mimicking the vasculogenic process. The aim of this study was to establish and characterize ten cell lines from canine mammary gland tumour according to immunophenotype and tumorigenicity, and with its ability to form vasculogenic mimicry-like structures in vitro. Fifteen samples from canine mammary gland carcinoma were collected and cultured in vitro and ten cell lines were established and characterized. Cells were evaluated for morphology, phenotype, vascular mimicry and tumorigenicity. All cell lines presented spindle shape morphology and expressed concomitant pan-cytokeratin and CK8/18. Four cell lines had vasculogenic mimicry ability and two cell showed in vivo tumorigenic potential. Cell characterization of those lines will help to create a database for more knowledge of mammary gland carcinomas in dogs, including studies of tumor behavior and new therapeutic targets.


PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180448 ◽  
Author(s):  
Ashley Case ◽  
Becky K. Brisson ◽  
Amy C. Durham ◽  
Suzanne Rosen ◽  
James Monslow ◽  
...  

2011 ◽  
Vol 21 (6) ◽  
pp. 1633-1636 ◽  
Author(s):  
Annahita Rezaie ◽  
Abbas Tavasoli ◽  
Mitra Mehrazma ◽  
Alireza Bahonar

Author(s):  
N M Triana ◽  
E Wilujeng ◽  
M W H Putri ◽  
D M P Yuda ◽  
A L Hardiono ◽  
...  

2014 ◽  
Vol 95 (6) ◽  
pp. 401-410 ◽  
Author(s):  
Peter Orendáš ◽  
Peter Kubatka ◽  
Bianka Bojková ◽  
Monika Kassayová ◽  
Karol Kajo ◽  
...  

2011 ◽  
Vol 23 (6) ◽  
pp. 1230-1233 ◽  
Author(s):  
Fabrizio Grandi ◽  
Marcia M. Colodel ◽  
Rafael M. Rocha ◽  
Julio L. Sequeira

Sebaceous metaplasia in canine mammary tumors is a rare condition with only 1 case documented. The current study describes a case of sebaceous metaplasia in a mammary gland carcinoma of an 8-year-old intact, nulliparous female Poodle dog with a subcutaneous tumor located in the left fifth mammary gland. The lesion measured 0.7 cm × 0.5 cm × 0.6 cm in diameter, was firm, circumscribed, painless, non-haired, and non-ulcerated, and did not adhere to deep tissues. The cut surface was non-lobulated, non-encapsulated, whitish to gray, and opaque. Histological evaluation revealed 3 different populations of cells: the first was composed of columnar to cuboidal malignant epithelial cells arranged in intraductal papillary projections, the second of myoepithelial cells associated with a myxoid stroma, and the third presenting sebaceous metaplasia similar to those previously described in both human and veterinary medicine.


2014 ◽  
Vol 45 (3) ◽  
pp. 682-685 ◽  
Author(s):  
Alonso Reyes Matute ◽  
Adriana Mendez Bernal ◽  
José Ramírez Lezama ◽  
Manzano Pech Linaloe Guadalupe ◽  
Galicia Avalos Marco Antonio

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