scholarly journals Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo

2019 ◽  
Author(s):  
Patricia de Faria Lainetti ◽  
Andressa Brandi ◽  
Antonio Fernando Leis Filho ◽  
Maria Carolina Mangini Prado ◽  
Priscila Emiko Kobayashi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Cell Lines ◽  
Vasculogenic Mimicry ◽  
Preclinical Model ◽  
Mammary Gland Carcinoma ◽  
Gland Carcinoma ◽  
Canine Mammary Gland

Mammary neoplasms affect a population of uncastrated and elderly female dogs and most of these neoplasms are malignant. In order to study this disease cell culture presents itself as a promising preclinical model, creating the opportunity to deposit cell lines at a cell bank, allowing a great repetition of the assays and making the validation of the results more reliable. Including, in vitro experiments for vasculogenic mimicry (VM) evaluation. VM is related to cancer cells capable of generate vascular-like structures without endothelial cells, mimicking the vasculogenic process. The aim of this study was to establish and characterize ten cell lines from canine mammary gland tumour according to immunophenotype and tumorigenicity, and with its ability to form vasculogenic mimicry-like structures in vitro. Fifteen samples from canine mammary gland carcinoma were collected and cultured in vitro and ten cell lines were established and characterized. Cells were evaluated for morphology, phenotype, vascular mimicry and tumorigenicity. All cell lines presented spindle shape morphology and expressed concomitant pan-cytokeratin and CK8/18. Four cell lines had vasculogenic mimicry ability and two cell showed in vivo tumorigenic potential. Cell characterization of those lines will help to create a database for more knowledge of mammary gland carcinomas in dogs, including studies of tumor behavior and new therapeutic targets.

scholarly journals Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo

2020 ◽  
Vol 7 ◽  
Author(s):  
Patrícia de Faria Lainetti ◽  
Andressa Brandi ◽  
Antonio Fernando Leis Filho ◽  
Maria Carolina Mangini Prado ◽  
Priscila Emiko Kobayashi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Vasculogenic Mimicry ◽  
Carcinoma Cell Lines ◽  
Gland Carcinoma ◽  
Canine Mammary Gland

TMOD-31. AN ORGANOTYPIC TISSUE PLATFORM TO BRIDGE IN VITRO AND IN VIVO ASSAYS FOR BRAIN CANCER TREATMENT

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi222-vi222
Author(s):  
Breanna Mann ◽  
Noah Bell ◽  
Denise Dunn ◽  
Scott Floyd ◽  
Shawn Hingtgen ◽  
...  
Keyword(s):  
Cell Lines ◽  
Brain Cancer ◽  
Brain Slice ◽  
Brain Slices ◽  
In Vitro Assays ◽  
Preclinical Model ◽  
Short Period ◽  
The Brain

Abstract Brain cancers remain one of the greatest medical challenges. The lack of experimentally tractable models that recapitulate brain structure/function represents a major impediment. Platforms that enable functional testing in high-fidelity models are urgently needed to accelerate the identification and translation of therapies to improve outcomes for patients suffering from brain cancer. In vitro assays are often too simple and artificial while in vivo studies can be time-intensive and complicated. Our live, organotypic brain slice platform can be used to seed and grow brain cancer cell lines, allowing us to bridge the existing gap in models. These tumors can rapidly establish within the brain slice microenvironment, and morphologic features of the tumor can be seen within a short period of time. The growth, migration, and treatment dynamics of tumors seen on the slices recapitulate what is observed in vivo yet is missed by in vitro models. Additionally, the brain slice platform allows for the dual seeding of different cell lines to simulate characteristics of heterogeneous tumors. Furthermore, live brain slices with embedded tumor can be generated from tumor-bearing mice. This method allows us to quantify tumor burden more effectively and allows for treatment and retreatment of the slices to understand treatment response and resistance that may occur in vivo. This brain slice platform lays the groundwork for a new clinically relevant preclinical model which provides physiologically relevant answers in a short amount of time leading to an acceleration of therapeutic translation.

In vitro and in vivo characterization of a novel hedgehog signaling antagonist in human glioblastoma cell lines

2012 ◽  
Vol 131 (2) ◽  
pp. E33-E44 ◽  
Author(s):  
Pietro Ferruzzi ◽  
Federica Mennillo ◽  
Antonella De Rosa ◽  
Cinzia Giordano ◽  
Marco Rossi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hedgehog Signaling ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
In Vivo Characterization ◽  
Glioblastoma Cell Lines

scholarly journals Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids

Virology ◽  
2008 ◽  
Vol 373 (2) ◽  
pp. 352-361 ◽  
Author(s):  
Z.Q. Yuan ◽  
E.A. Gault ◽  
P. Gobeil ◽  
C. Nixon ◽  
M.S. Campo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Model Systems ◽  
Fibroblast Cell Lines

scholarly journals Development and Characterization of siRNA Lipoplexes: Effect of Different Lipids, In Vitro Evaluation in Cancerous Cell Lines and In Vivo Toxicity Study

2014 ◽  
Vol 15 (6) ◽  
pp. 1630-1643 ◽  
Author(s):  
Nirav Khatri ◽  
Dipesh Baradia ◽  
Imran Vhora ◽  
Mohan Rathi ◽  
Ambikanandan Misra
Keyword(s):  
Cell Lines ◽  
Toxicity Study ◽  
In Vitro Evaluation ◽  
In Vivo Toxicity ◽  
Cancerous Cell

A novel orthotopic animal model of human chondrosarcoma

2012 ◽  
Vol 21 (04) ◽  
pp. 296-300
Author(s):  
K. Horas ◽  
M. Tonak ◽  
A. A. Kurth
Keyword(s):  
Animal Model ◽  
Cell Lines ◽  
Bone Tumour ◽  
In Vivo Studies ◽  
Preclinical Model ◽  
Orthotopic Implantation ◽  
Chondrosarcoma Cell ◽  
Human Chondrosarcoma

SummaryChondrosarcoma is the second most common primary malignant bone tumour in humans. Currently, surgical resection is the only appropriate curative approach as it is relatively unresponsive to traditional chemoand radiotherapy. However, a complete resection is often hindered due to the proximity to organs resulting in a poor outcome of this challenging malignancy. Few novel antitumour agents have been tested on different chondrosarcoma cell lines in vitro so far. In order to qualify new agents in vivo, animal models are often used in which cell lines are subcutaneously injected prior to chemotherapeutical treatment. These types of models often lack relevance to the human chondrosarcoma as the number of agents that fail in the clinic far outweighs those considered effective on in vivo studies. Orthotopic xenograft models however are of much more predictive value. Thus, the development of a novel orthotopic animal model for human chondrosarcoma using a three-dimensional matrix carrying tumour cells, was the aim of this study. For that purpose, SW-1353, a human bone chondrosarcoma cell line, was first cultured in MatrigelTM, followed by orthotopic implantation into10 SCID mice by intra-tibial injection. After 40 days, the animals developed localized bone tumours verified by radiographic and histological examinations. Radiologic and histological sections showed osteolysis and invasive tumour growth. This study demonstrates a promising new method for effective and reproducible orthotopic implantation of human chondrosarcoma. The presented animal model allows further examination and can be used as a predictive preclinical model for anticancer drug activity in humans.

Grading in Canine Mammary Gland Carcinoma

2009 ◽  
Vol 9 (4) ◽  
pp. 333-338 ◽  
Author(s):  
A. Rezaie ◽  
A. Tavasoli ◽  
A. Bahonar ◽  
M. Mehrazma
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Carcinoma ◽  
Gland Carcinoma ◽  
Canine Mammary Gland

Immuno-histochemical staining expression of Ki67 protein in canine mammary gland carcinoma

2010 ◽  
Vol 20 (6) ◽  
pp. 557-561 ◽  
Author(s):  
Annahita Rezaie ◽  
Abbas Tavasoli ◽  
Mitra Mehrazma ◽  
Alireza Bahonar
Keyword(s):  
Mammary Gland ◽  
Histochemical Staining ◽  
Mammary Gland Carcinoma ◽  
Gland Carcinoma ◽  
Canine Mammary Gland

scholarly journals FKBPL-based peptide, ALM201, targets angiogenesis and cancer stem cells in ovarian cancer

2019 ◽  
Vol 122 (3) ◽  
pp. 361-371 ◽  
Author(s):  
Stephanie Annett ◽  
Gillian Moore ◽  
Amy Short ◽  
Andrea Marshall ◽  
Cian McCrudden ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Vasculogenic Mimicry ◽  
Tissue Microarrays ◽  
Protective Role ◽  
Growth Delay ◽  
Protein Levels ◽  
Tumour Growth Delay

Abstract Background ALM201 is a therapeutic peptide derived from FKBPL that has previously undergone preclinical and clinical development for oncology indications and has completed a Phase 1a clinical trial in ovarian cancer patients and other advanced solid tumours. Methods In vitro, cancer stem cell (CSC) assays in a range of HGSOC cell lines and patient samples, and in vivo tumour initiation, growth delay and limiting dilution assays, were utilised. Mechanisms were determined by using immunohistochemistry, ELISA, qRT-PCR, RNAseq and western blotting. Endogenous FKBPL protein levels were evaluated using tissue microarrays (TMA). Results ALM201 reduced CSCs in cell lines and primary samples by inducing differentiation. ALM201 treatment of highly vascularised Kuramochi xenografts resulted in tumour growth delay by disruption of angiogenesis and a ten-fold decrease in the CSC population. In contrast, ALM201 failed to elicit a strong antitumour response in non-vascularised OVCAR3 xenografts, due to high levels of IL-6 and vasculogenic mimicry. High endogenous tumour expression of FKBPL was associated with an increased progression-free interval, supporting the protective role of FKBPL in HGSOC. Conclusion FKBPL-based therapy can (i) dually target angiogenesis and CSCs, (ii) target the CD44/STAT3 pathway in tumours and (iii) is effective in highly vascularised HGSOC tumours with low levels of IL-6.

Sign in / Sign up

Export Citation Format

Share Document