Determination of the activity of hydrogen peroxide scavenging by using blue-emitting glucose oxidase–stabilized gold nanoclusters as fluorescent nanoprobes and a Fenton reaction that induces fluorescence quenching

2017 ◽  
Vol 184 (4) ◽  
pp. 1103-1108 ◽  
Author(s):  
Wanwan Cui ◽  
Haiyan Qin ◽  
Yang Zhou ◽  
Jianxiu Du
The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7650-7656 ◽  
Author(s):  
Hao-Hua Deng ◽  
Gang-Wei Wu ◽  
Dong He ◽  
Hua-Ping Peng ◽  
Ai-Lin Liu ◽  
...  

In this study, we used Fenton reaction-stimulated fluorescence quenching of N-acetyl-l-cysteine-protected gold nanoclusters as a reporter system for the determination of H2O2, glucose, and catalase.


2012 ◽  
Vol 538-541 ◽  
pp. 2434-2437 ◽  
Author(s):  
Bao Shan He ◽  
Na Gao ◽  
Fang Wei ◽  
Qi Yu Lu

In the presence of glucose oxidase, glucose in samples was oxygenated to hydrogen peroxide, the solution turned from colourless to yellow upon the reaction of potassium titanyl oxalate to the generated hydrogen peroxide. Using ultraviolet spectrophotometry, a new optical method for detecting glucose in amylofermentation liquid has been established. Results demonstrated that glucose concentrations were proportional to absorbance at the maximum absorption wavelength of 380 nm. A favorable linearity was presented in the range of 1 mmol/L to 60 mmol/L. The linear coeffciency was 0.993. This method was simple, reliable, and could be used for determing glucose in samples.


2019 ◽  
Vol 68 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Ryuta UEKI ◽  
Ryota KATO ◽  
Yoshitaka IMAIZUMI ◽  
Yoko IWAMOTO ◽  
Waqar A. JADOON ◽  
...  

1961 ◽  
Vol 14 (3) ◽  
pp. 489 ◽  
Author(s):  
MA Jermyn

Youatt (1958) has described a method for estimating cellobiase activity that uses the coupled glucose oxidase-catalase system to determine manometrically the amount of glucose liberated from cellobiose by the enzyme. The high specificity of glucose oxidase makes it the reagent of choice for the otherwise difficult determination of glucose in the presence of excess cellobiose. The hydrogen peroxide produced by glucose oxidase can, however, also be demonstrated qualitatively by the use of peroxidase and both Eyer, Linzenmeier, and von Schrader (1957) and Huggett and Nixon (1957) have described sensitive quantitative glucose oxidase-peroxidase systems for determining glucose as such.


2008 ◽  
Vol 627 (2) ◽  
pp. 270-276 ◽  
Author(s):  
Emmanuel F. Olasehinde ◽  
Shinya Makino ◽  
Hiroaki Kondo ◽  
Kazuhiko Takeda ◽  
Hiroshi Sakugawa

2016 ◽  
Vol 184 (3) ◽  
pp. 677-686 ◽  
Author(s):  
Shuang Zhao ◽  
Jun Zhang ◽  
Zhengping Li ◽  
Peixin Zhang ◽  
Yunxiao Li ◽  
...  

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