Serological and molecular variability of watermelon mosaic virus (genus Potyvirus)

2007 ◽  
Vol 152 (4) ◽  
pp. 775-781 ◽  
Author(s):  
C. Desbiez ◽  
C. Costa ◽  
C. Wipf-Scheibel ◽  
M. Girard ◽  
H. Lecoq
Keyword(s):  
Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Molecular Variability

Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran

Virus Genes ◽  
2008 ◽  
Vol 37 (3) ◽  
pp. 304-313 ◽  
Author(s):  
Maraym Sharifi ◽  
Hossain Massumi ◽  
Jahangir Heydarnejad ◽  
Akbar Hosseini Pour ◽  
Mehdi Shaabanian ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Molecular Variability ◽  
Virus Isolates

Molecular variability of watermelon mosaic virus isolates from Argentina

2020 ◽  
Vol 156 (4) ◽  
pp. 1091-1099
Author(s):  
E. Pozzi ◽  
M. C. Perotto ◽  
S. Bertin ◽  
A. Manglli ◽  
C. Luciani ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Molecular Variability ◽  
Virus Isolates

scholarly journals Multiple aromatic amino acids are involved in potyvirus movement by forming π-stackings to maintain coat protein accumulation

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Zhi-Yong Yan ◽  
Xiao-Jie Xu ◽  
Le Fang ◽  
Chao Geng ◽  
Yan-Ping Tian ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Infectious Clone ◽  
Three Dimensional ◽  
Cell Movement ◽  
Systemic Infection ◽  
Watermelon Mosaic Virus ◽  
Protein Accumulation ◽  
Three Dimensional Model ◽  
Aromatic Residues

AbstractCoat protein (CP) is required for potyviruses to move and establish a systemic infection in plants. π-stackings formed by aromatic residues play critical roles in maintaining protein stability and functions. As we know, many aromatic residues located in the core region of potyvirus CPs are conserved. However, their roles in potyvirus infection remain largely unknown. Here, through analysis of the three-dimensional model of the tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) CP, 16 aromatic residues were predicated to form π-stackings. The results of transient expression experiments demonstrated that deletion of any of these 16 aromatic residues reduced CP accumulation. Infectivity assays showed that deletion of any of these aromatic residues in the TVBMV infectious clone abolished cell-to-cell movement and reduced replication of the virus. Substitution of Y105 and Y147 individually with non-aromatic residues alanine or glycine reduced CP accumulation, virus replication, and abolished the ability of TVBMV to move intercellularly, while substitution of these two residues individually with aromatic residues phenylalanine or tryptophan, had no or little effect on CP accumulation and TVBMV systemic movement and replication. Similar results were obtained from the CP mutants of watermelon mosaic virus (WMV, genus Potyvirus). Taken together, our results demonstrate that multiple aromatic residues in CP are involved in potyvirus movement by forming π-stackings to maintain CP accumulation.

scholarly journals High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 53
Author(s):  
Vivek Khanal ◽  
Harrington Wells ◽  
Akhtar Ali
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
High Prevalence ◽  
The Us ◽  
Close Relationship ◽  
The Status ◽  
Low Incidence

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

scholarly journals Nature, Incidence, and Symptomatology of Viruses Infecting Vanilla tahitensis in French Polynesia

Plant Disease ◽  
2004 ◽  
Vol 88 (2) ◽  
pp. 119-124 ◽  
Author(s):  
M. Grisoni ◽  
F. Davidson ◽  
C. Hyrondelle ◽  
K. Farreyrol ◽  
M. L. Caruana ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Significant Proportion ◽  
French Polynesia ◽  
Bean Common Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Cymbidium Mosaic Virus ◽  
Virus Identification ◽  
Society Islands ◽  
Leeward Islands

A survey was carried out to identify the viruses infecting vanilla in French Polynesia and to assess their incidence. Virus identification was based on enzyme-linked immunosorbent assay (ELISA) and, for potyviruses, on the sequence of part of the coat protein and inoculation assays. Between 1998 and 1999, 3,610 vanilla plants from 49 plots in the Society Islands were indexed. Cymbidium mosaic virus (CymMV) was detected in 500 vines from 10 plots in the Leeward Islands. The data suggest that this virus has spread widely since its first detection in French Polynesia in 1986, most likely through the dissemination of symptomless infected cuttings. Viruses belonging to the Potyvirus genus were found in 674 plants from 27 plots in the four islands surveyed. Three distinct potyviruses have been identified: (i) Vanilla mosaic virus, (ii) Watermelon mosaic virus, and (iii) and a virus related to Bean common mosaic virus. The symptoms induced on Vanilla tahitensis by the three potyviruses can be differentiated from each other and from those due to CymMV. A significant proportion of the plants surveyed (97/476) were symptomatic but tested negative by ELISA for CymMV and the Potyvirus group. Odontoglossum ringspot virus was not detected in any sample tested.

scholarly journals Sintomas atípicos em frutos de meloeiro e de melancia ocasionados por Watermelon mosaic virus-2

2002 ◽  
Vol 27 (5) ◽  
pp. 546-546 ◽  
Author(s):  
J. ALBÉRSIO A. LIMA ◽  
M. ABÍLIO DE QUEIROZ ◽  
NAJARA F. RAMOS ◽  
M. FÁTIMA B. GONÇALVES
Keyword(s):  
Mosaic Virus ◽  
Watermelon Mosaic Virus

scholarly journals Herança da resistência ao Watermelon mosaic virus em melancia (Citrullus lanatus L.)

2006 ◽  
Vol 31 (3) ◽  
pp. 302-305 ◽  
Author(s):  
J. Evando A. Beserra Júnior ◽  
Wilson R. Maluf ◽  
Antonia R. Figueira ◽  
Beatriz M. Barguil
Keyword(s):  
Mosaic Virus ◽  
Citrullus Lanatus ◽  
Watermelon Mosaic Virus

Foi estudado o controle genético da resistência do genótipo não-comercial de melancia, PI 595201, ao vírus do mosaico da melancia (Watermelon mosaic virus, WMV). Avaliaram-se os genitores, a cultivar Crimson Sweet (P1 - suscetível) e a introdução PI 595201 (P2 - resistente), bem como as populações F1, F2, e os retrocruzamentos para ambos os parentais, RC11 (F1 x P1) e RC12 (F1 x P2). A severidade dos sintomas, depois da inoculação mecânica com WMV, foi avaliada de acordo com uma escala de notas de 1 (folhas sem sintomas) a 5 (mosaico intenso e deformações foliares). A cultivar Crimson Sweet apresentou média geral acima de 4,0, enquanto a introdução PI 595201 apresentou média 1,0, confirmando a reação contrastante das linhagens parentais. A hipótese de herança monogênica foi rejeitada, mostrando ser a resistência da introdução PI 595201 de controle oligo ou poligênico, com indicativo de dominância completa no sentido de maior resistência ao vírus. A estimativa de herdabilidade no sentido amplo foi acima de 0,8. A estimativa do número de genes, controlando o caráter, foi 4,16. O modelo aditivo-dominante é sugerido para explicar o controle genético da resistência.

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