scholarly journals Nature, Incidence, and Symptomatology of Viruses Infecting Vanilla tahitensis in French Polynesia

Plant Disease ◽  
2004 ◽  
Vol 88 (2) ◽  
pp. 119-124 ◽  
Author(s):  
M. Grisoni ◽  
F. Davidson ◽  
C. Hyrondelle ◽  
K. Farreyrol ◽  
M. L. Caruana ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Significant Proportion ◽  
French Polynesia ◽  
Bean Common Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Cymbidium Mosaic Virus ◽  
Virus Identification ◽  
Society Islands ◽  
Leeward Islands

A survey was carried out to identify the viruses infecting vanilla in French Polynesia and to assess their incidence. Virus identification was based on enzyme-linked immunosorbent assay (ELISA) and, for potyviruses, on the sequence of part of the coat protein and inoculation assays. Between 1998 and 1999, 3,610 vanilla plants from 49 plots in the Society Islands were indexed. Cymbidium mosaic virus (CymMV) was detected in 500 vines from 10 plots in the Leeward Islands. The data suggest that this virus has spread widely since its first detection in French Polynesia in 1986, most likely through the dissemination of symptomless infected cuttings. Viruses belonging to the Potyvirus genus were found in 674 plants from 27 plots in the four islands surveyed. Three distinct potyviruses have been identified: (i) Vanilla mosaic virus, (ii) Watermelon mosaic virus, and (iii) and a virus related to Bean common mosaic virus. The symptoms induced on Vanilla tahitensis by the three potyviruses can be differentiated from each other and from those due to CymMV. A significant proportion of the plants surveyed (97/476) were symptomatic but tested negative by ELISA for CymMV and the Potyvirus group. Odontoglossum ringspot virus was not detected in any sample tested.

scholarly journals Occurrence and Distribution of Pumpkin and Squash Viruses in Illinois

Plant Disease ◽  
2008 ◽  
Vol 92 (1) ◽  
pp. 61-68 ◽  
Author(s):  
S. Jossey ◽  
M. Babadoost
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Dual Infection ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Virus Infections ◽  
Dark Green

Surveys were conducted during 2004 to 2006 to identify the viruses infecting pumpkin and squash in Illinois. In 2004, 16 jack-o-lantern pumpkin (Cucurbita pepo) samples and one squash (C. pepo) sample were collected from 11 counties. In 2005, 85 jack-o-lantern pumpkin, 12 processing pumpkin (Cucurbita moschata), 37 squash, and six gourd (C. pepo) samples were collected from 54 counties. In 2006, 85 jack-o-lantern pumpkin, 16 processing pumpkin, 51 squash, and 18 gourd samples were collected from 47 counties. Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), Squash mosaic virus (SqMV), Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV), and unknown potyviruses were detected in pumpkin, squash, and gourd fields during the surveys, using enzyme-linked immunosorbent assay (ELISA). Overall, 86, 11, 75, and 79% of jack-o-lantern pumpkin, processing pumpkin, squash, and gourds, respectively, were tested positive for virus infection during the survey. WMV was detected in 47, 46, and 52% of the samples in 2004, 2005, and 2006, respectively, and was the most prevalent virus throughout the state. SqMV was detected in more counties than any other virus because it was identified in 65 and 88% of the counties surveyed in 2005 and 2006, respectively. SqMV was detected in 6, 41, and 48% of the samples in 2004, 2005, and 2006, respectively. During the surveys, CMV was detected in 6, 4, and 3% of the samples; PRSV was detected in 6, 11, and 4% of the samples; and ZYMV was detected in 18, 4, and 4% of the samples tested in 2004, 2005, and 2006, respectively. TRSV was detected in 3% of the samples in 2005, for the first time on pumpkin in Illinois. Pathogenicity of the detected viruses was proved for CMV, PRSV, SqMV, WMV, and ZYMV on summer squash (‘Fortune’ and ‘Grey Zucchini’), jack-o-lantern pumpkin (‘Howden’), and processing pumpkin (‘Dickinson’). All of the viruses were present alone and mixed in the samples tested. Earlier in the growing seasons (July and early August), single-virus infections were detected. Mixed infections were more common from the second week of August until the end of the growing season in October. Dual infection of WMV and SqMV was the most prevalent mixed virus infection detected in Illinois. Most viruses infecting pumpkin and squash showed similar symptoms. The most common symptoms observed in the commercial fields and in the greenhouse studies were light- and dark-green mosaic, veinbanding, veinclearing, puckering, and deformation of leaves of pumpkin, squash, and gourds. Severe symptoms included fernleaf and shoestring on leaves and color breaking and deformation of fruit.

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

2005 ◽  
Vol 95 (9) ◽  
pp. 1037-1042 ◽  
Author(s):  
Richard C. Larsen ◽  
Phillip N. Miklas ◽  
Keri L. Druffel ◽  
Stephen D. Wyatt
Keyword(s):  
Amino Acids ◽  
Mosaic Virus ◽  
Molecular Size ◽  
Bean Common Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Virus ◽  
Bean Plants ◽  
Recombination Point ◽  
Russian Strain ◽  
Polymerase Chain

A strain of Bean common mosaic necrosis virus (BCMNV) from Idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the NL-3 D strain (of Drifjhout) by reaction of differential bean cultivars. However, this BCMNV strain (designated NL-3 K) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. The nucleotide sequence encoding the predicted polyprotein of NL-3 K was 9,893 nucleotides (nt) in length, yielding a peptide with a molecular size of 362.1 kDa compared with a 9,626-nt, 350.9-kDa polyprotein for NL-3 D. Sequence analysis of the putative P1 protein suggests that the NL-3 K strain is a recombinant between NL-3 D and the Russian strain (RU1) of Bean common mosaic virus. The P1 protein of NL-3 K consisted of 415 amino acids compared with 317 for NL-3 D. The first 114 predicted amino acids of the NL-3 K P1 region were 98% identical with RU1. The remaining 301 amino acids of the protein shared only 34% identity with RU1 but were 98% identical with NL-3 D. Primers were designed that flanked the recombination point in the P1 coding sequence of NL-3 K. An amplicon of the expected size was produced by reverse-transcriptase polymerase chain reaction of total nucleic acid extracts of bean plants inoculated with NL-3 K, but not from those with NL-3 D or RU1. The increased symptom severity on selected common bean lines induced by NL-3 K suggests that the P1 gene may play a significant role in pathogenicity and virulence.

scholarly journals Resistance to the Emerging Moroccan Watermelon Mosaic Virus in Squash

2019 ◽  
Vol 109 (5) ◽  
pp. 895-903 ◽  
Author(s):  
Manuel Miras ◽  
Miguel Juárez ◽  
Miguel A. Aranda
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Genomic Structure ◽  
Severe Infection ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Amino Acid Substitutions ◽  
Multiple Sequence

Moroccan watermelon mosaic virus (MWMV) represents an emerging threat to cucurbit production in the Mediterranean Basin. We sequenced the near complete genome of MWMV-SQ10_1.1, a cloned Spanish isolate. MWMV-SQ10_1.1 has the typical potyvirus genomic structure, and phylogenetic analysis showed that it shared a common ancestor with other Mediterranean MWMV isolates. We used MWMV SQ10_1.1 to inoculate plants in a collection of commercial squash cultivars, including some described as potyvirus resistant. All inoculated plants from all cultivars showed severe infection symptoms. Twenty-four Cucurbita spp. accessions were then tested for their susceptibility to MWMV-SQ10_1.1. Plants of the C. ecuadorensis PI 432441 accession showed no symptoms and their enzyme-linked immunosorbent assay readings were similar to uninfected controls. Progeny analysis of F1 and F2 populations suggested that two recessive genes control PI 432441 resistance to MWMV. We hypothesized that this resistance could be associated with alleles of genes encoding the eukaryotic translation initiation factor 4E (eIF4E), particularly after determination of its recessive nature. A multiple sequence alignment including the two eIF4E ortholog sequences from PI 432441 (CeeIF4E1 and CeeIF4E2) identified three amino acid substitutions in CeeIF4E1 and two amino acid substitutions in CeeIF4E2 potentially involved in potyvirus resistance. Polymerase chain reaction markers for CeeIF4E1 and CeeIF4E2 were developed and used to genotype 156 F2 individuals already phenotyped; this analysis did not support an association of either CeeIF4E2 or CeeIF4E1 with MWMV resistance.

scholarly journals Occurrence, Distribution, and Relative Incidence of Seven Viruses Infecting Greenhouse-Grown Cucurbits in Iran

Plant Disease ◽  
2007 ◽  
Vol 91 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Hossain Massumi ◽  
Asghar Samei ◽  
Akbar Hosseini Pour ◽  
Mehdi Shaabanian ◽  
Heshmetollah Rahimian
Keyword(s):  
Mosaic Virus ◽  
Natural Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Fruit Samples ◽  
Cucumber Necrosis Virus ◽  
Papaya Ring Spot Virus ◽  
Wilt Virus

Greenhouse-grown cucurbit crops in several Iranian regions were surveyed for the incidence of Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ring spot virus-type W (PRSV-W), Watermelon mosaic virus-2 (WMV-2), Zucchini yellow mosaic virus (ZYMV), Cucumber necrosis virus (CuNV) and Tomato spotted wilt virus (TSWV) from September 2002 to June 2004. In all, 1,304 random and 1,085 symptomatic leaf or fruit samples were collected. Samples were analyzed for virus infection by enzyme-linked immunosorbent assay. CMV and ZYMV were the viruses most frequently detected, accounting for 21.2 and 18% of the infected plants, respectively. WMV-2 was detected with 4.3% incidence in 15 regions and TSWV with 1.25% incidence only in 2 regions. CuNV, SqMV, and PRSV-W were not detected in any samples. Double and triple infections involving different combinations of CMV, ZYMV, WMV-2, and TSWV were noted in 117 and 4 samples, respectively. Natural infection of cucumber with TSWV and ZYMV is reported for the first time from Iran. Of 16 plant species from 14 genera, growing in or around greenhouse facilities, 6 were found infected with ZYMV, TSWV, WMV-2, and CMV and may act as reservoirs of the viruses. Four species are reported as new hosts of these viruses.

scholarly journals Cucumber green mottle mosaic virus in agroecosystems of Ukraine

2018 ◽  
Vol 76 (2) ◽  
pp. 71-78
Author(s):  
T. Rudnieva ◽  
T. Shevchenko ◽  
A. Shevchenko ◽  
I. Budzanivska
Keyword(s):  
Mosaic Virus ◽  
Open Field ◽  
Zucchini Yellow Mosaic Virus ◽  
Field Conditions ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Vegetable Crops ◽  
Seed Material ◽  
Planting Material

Cucumber green mottle mosaic virus (CGMMV) is a widespread pathogen capable of infecting plants cultivated in both greenhouse and openfield conditions with equal efficiency. The host range of CGMMV is restricted to cucurbit plants whereas induced crop losses may reach 25–50 %. Despite the wide array of available antiviral techniques, CGMMV could not be completely removed from the seed/planting material. In Ukraine, Cucumber green mottle mosaic virus occurs almost exclusively on greenhouse cucumbers. However, data from other countries suggest wide spread of CGMMV on watermelons, melons, cucumbers and squashes grown in both greenhouse and open-field conditions. In this view and taking into account high pathogenicity of CGMMV, we aimed at studying spread of Cucumber green mottle mosaic virus in Ukraine on cucurbit plants in the open-field conditions. Methods: enzyme-linked immunosorbent assay, electron microscopy, statistical methods. Results : Spread of Cucumber green mottle mosaic virus (CGMMV) on cucurbit vegetable crops grown in open-field conditions was analyzed in Ukraine. It was shown that CGMMV is an extremely rare pathogen in open-field conditions in our country. Duringthelast 8 years we have confirmed only several cases of CGMMV infection on cucumbers, squash, pumpkin and melon in Kyiv and Poltava regions. Additionally, half of these cases were mixed infection of 2–3 viruses. From 250 collected samples, CGMMV was detected in 5 plants totaling to only 2,4 %. Importantly, monoinfection of CGMMV was shown exclusively on cucumbers and watermelons, whereas melons and squashes were mixed infected. Melons were typically infected with CGMMV and Watermelon mosaic virus 2 (WMV 2) or with WMV 2 and Zucchini yellow mosaic virus (ZYMV). Squash plants were infected with CGMMV and Cucumber mosaic virus (CMV).Consequently, we have checked commercially available seeds of cucumber, squash, zucchini, melon and watermelon from different producers in Ukraine for virus contamination and have confirmed the occurrence of CGMMV in some seed batches. Conclusions: Long-term observations in Ukraine showed that Cucumber green mottle mosaic virus infected mostly cucumbers cultivated in greenhouses and was only rarely found in cucurbits grown in open-field conditions. Screening of commercially available seed material indicated that 16 % of it was contaminated with CGMMV. Basing on available data, it is presumed that virus-contaminated seed material may pose a major threat of CGMMV occurrence and spread on cucurbits cultivated in open-field conditions.

scholarly journals Inheritance of Resistance to the Watermelon Strain of Papaya Ringspot Virus in the Cucumber Line TMG-1

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 338-340 ◽  
Author(s):  
T. Wai ◽  
R. Grumet
Keyword(s):  
Mosaic Virus ◽  
Dominant Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Genetics Of Resistance ◽  
Elisa Data

The inbred cucumber (Cucumis sativus L.) line TMG-1 is resistant to three potyviruses: zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), and the watermelon strain of papaya ringspot virus (PRSV-W). In this study we sought to determine the genetics of resistance to PRSV-W. TMG-1 was crossed with WI-2757, an inbred line susceptible to all three viruses. Segregation data indicated that resistance to PRSV-W was due to a single dominant gene (proposed designation, Prsv-2). Enzyme-linked immunosorbent assay (ELISA) data suggested that the mechanism of resistance to PRSV-W differs from that for ZYMV and WMV, and may be better described as tolerance. Although the plants were free of symptoms, high PRSV-W titers existed in young expanding leaves of the TMG-1 plants and the WI-2757 × TMG-1 F1 progeny.

scholarly journals Biological, Serological and Molecular Characterisation of a New Virus Species Infecting Telfairia occidentalis in Calabar, Cross River State, Nigeria

2020 ◽  
pp. 23-33
Author(s):  
O. I. Eyong ◽  
A. T. Owolabi ◽  
A. A. J. Mofunanya ◽  
E. E. Ekpiken
Keyword(s):  
Mosaic Virus ◽  
Virus Isolate ◽  
Diagnostic Tools ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Virus Species ◽  
Cross River ◽  
Cross River State ◽  
Telfairia Occidentalis ◽  
Important Cash Crop

Telfairia occidentalis (Hook) belongs to the family Cucurbitaceae and is an economically important cash crop worldwide. It is widely cultivated in Nigeria including the South Eastern part of the country. This research was aimed at isolating, characterising and identifying a new virus species infecting Telfairia occidentalis in Calabar, Cross River State. Diagnostic tools employed included host range/symptomatology, insect transmission test, Antigen Coated Plate (ACP) Enzyme Linked-Immunosorbent Assay (ELISA) and gene sequencing. Results revealed that the virus isolate infected only members of the cucurbit family producing rugosity, mosaic, mottle and leaf malformation/deformation. The virus isolate was transmitted by Aphis spiraecola in a fore-gut manner and not by A. citricida. It reacted positively against universal potyvirus antiserum. Sequence analysis showed that the Telfairia occidentalis virus isolate had 75% sequence identity with Moroccan watermelon mosaic virus (MWM) which was found to be the closest. The virus was consequently considered a new species of potyvirus for which the name Telfairia severe mosaic virus (TeSMV) was suggested.

Cymbidium mosaic virus. [Distribution map].

2020 ◽  
Author(s):  
Keyword(s):  
New York ◽  
South Carolina ◽  
Mosaic Virus ◽  
New Caledonia ◽  
French Polynesia ◽  
Distribution Map ◽  
Cymbidium Mosaic Virus ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Cymbidium mosaic virus. Alphaflexiviridae: Potexvirus. Hosts: Orchidaceae, especially vanilla (Vanilla planifolia), Cattleya, Cymbidium and Phalaenopsis spp. Information is given on the geographical distribution in Europe (Belgium, Hungary, Lithuania, Poland, Ukraine), Asia (China, Fujian, Guangdong, Hainan, Yunnan, Zheijiang, India, Himachal Pradesh, Karnataka, Kerala, Sikkim, West Bengal, Indonesia, Japan, Korea Republic, Malaysia, Singapore, Taiwan, Thailand, Vietnam), Africa (Madagascar, Reunion), North America (Canada, Alberta, British Columbia, Ontario, Quebec, Mexico, USA, California, Colorado, Florida, Hawaii, Louisiana, Missouri, Nebraska, New Hampshire, New York, Pennsylvania, South Carolina, Texas, Virginia), Central America & Caribbean (Costa Rica, Guadeloupe, Puerto Rico), South America (Argentina, Brazil, Rio de Janeiro, Sao Paulo, Colombia, Paraguay, Venezuela), Oceania (Australia, Northern Territory, Cook Islands, Fiji, French Polynesia, Guam, New Caledonia, New Zealand, Niue, Tonga, Vanuatu).

scholarly journals Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

2001 ◽  
Vol 26 (1) ◽  
pp. 90-92 ◽  
Author(s):  
MARIA C. C. L. MOURA ◽  
J. ALBERSIO A. LIMA ◽  
VANÚZIA B. OLIVEIRA ◽  
M. FÁTIMA B. GONÇALVES
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Moschata

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

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