Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

2015 ◽  
Vol 160 (11) ◽  
pp. 2727-2739 ◽  
Author(s):  
Diana Leibman ◽  
Shanmugam Prakash ◽  
Dalia Wolf ◽  
Aaron Zelcer ◽  
Ghandi Anfoka ◽  
...  
Keyword(s):  
Small Rna ◽  
Tomato Yellow Leaf ◽  
Transgenic Tomato ◽  
Yellow Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

Truncated Rep gene originated from Tomato yellow leaf curl virus-Israel [Mild] confers strain-specific resistance in transgenic tomato

2004 ◽  
Vol 144 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Y ANTIGNUS ◽  
R VUNSH ◽  
O LACHMAN ◽  
M PEARLSMAN ◽  
L MASLENIN ◽  
...  
Keyword(s):  
Specific Resistance ◽  
Tomato Yellow Leaf ◽  
Transgenic Tomato ◽  
Yellow Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

Transgenic Tomato Plants Expressing the Tomato Yellow Leaf Curl Virus Capsid Protein are Resistant to the Virus

1994 ◽  
Vol 12 (5) ◽  
pp. 500-504 ◽  
Author(s):  
Talya Kunik ◽  
Raffi Salomon ◽  
Daniel Zamir ◽  
Nir Navot ◽  
Muhammad Zeidan ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Tomato Yellow Leaf ◽  
Transgenic Tomato ◽  
Yellow Leaf ◽  
Virus Capsid ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Leaf Curl Virus ◽  
Virus Capsid Protein ◽  
Leaf Curl

Intron–hairpin RNA Derived from Replication Associated Protein C1 Gene Confers Immunity to Tomato Yellow Leaf Curl Virus Infection in Transgenic Tomato Plants

2006 ◽  
Vol 15 (3) ◽  
pp. 291-304 ◽  
Author(s):  
Alejandro Fuentes ◽  
Pedro L. Ramos ◽  
Elvira Fiallo ◽  
Danay Callard ◽  
Yadira Sánchez ◽  
...  
Keyword(s):  
Virus Infection ◽  
Tomato Yellow Leaf ◽  
Transgenic Tomato ◽  
Yellow Leaf ◽  
Hairpin Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals The tomato yellow leaf curl virus C4 protein alters the expression of plant developmental genes correlating to leaf upward cupping phenotype in tomato

2021 ◽  
Author(s):  
Chellappan Padmanabhan ◽  
Yi Zheng ◽  
Md Shamimuzzaman ◽  
Jennifer R. Wilson ◽  
Zhangjun Fei ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Tomato Yellow Leaf ◽  
Transgenic Tomato ◽  
Disease Symptom ◽  
Yellow Leaf ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Leaf Curl Virus ◽  
Leaf Curl

AbstractTomato yellow leaf curl virus (TYLCV), a monopartite begomovirus in the family Geminiviridae, is efficiently transmitted by the whitefly, Bemisia tabaci, and causes serious economic losses to tomato crops around the world. TYLCV-infected tomato plants develop distinctive symptoms of yellowing and leaf upper cupping. In recent years, excellent progress has been made in the characterization of TYLCV C4 protein function as a pathogenetic determinant in experimental plants, including Nicotiana benthamiana and Arabidopsis thaliana. However, molecular mechanism leading to disease symptom development in natural host plant tomato has yet to be characterized. The aim of the current study was to generate transgenic tomato plants expressing the TYLCV C4 gene and evaluate differential gene expression through comparative transcriptome analysis between the transgenic C4 plants and the transgenic green fluorescent protein (Gfp) gene control plants. Transgenic tomato plants expressing the TYLCV C4 developed phenotypes, including leaf upward cupping and yellowing that are similar the disease symptom expressed on tomato plants infected with TYLCV. In a total of 241 differentially expressed genes identified in the transcriptome analysis, a series of plant development-related genes, including transcription factors, glutaredoxins, protein kinases, R-genes and microRNA target genes, were significantly altered. These results provide further evidence to support the important function of the C4 protein in begomovirus pathogenicity. These transgenic tomato plants could serve as basic genetic materials for further characterization of plant receptors that are interacting with the TYLCV C4.

Control of the Bemisia tabaci/Tomato yellow leaf curl virus complex on protected tomato crops in Algarve (Portugal)*

EPPO Bulletin ◽  
2002 ◽  
Vol 32 (1) ◽  
pp. 31-35
Author(s):  
A. F. Arsenio ◽  
E. Neto ◽  
N. Ramos ◽  
S. Mangerico ◽  
E. Fortunato ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Virus Complex ◽  
Leaf Curl Virus ◽  
Leaf Curl

Study of a collection of tomato hybrids with genetic resistance to the yellow leaf curl virus in Southern Russia

2020 ◽  
pp. 30-34
Author(s):  
С.Ф. Гавриш ◽  
Т.А. Редичкина ◽  
А.В. Буц ◽  
Г.М. Артемьева
Keyword(s):  
Resistance Genes ◽  
Molecular Genetic Analysis ◽  
Tomato Yellow Leaf ◽  
Tomato Mosaic Virus ◽  
Average Weight ◽  
Yellow Leaf ◽  
The South ◽  
Economically Valuable Traits ◽  
Leaf Curl Virus ◽  
Leaf Curl

Дана информация об изучении коллекции гибридов F1томата (Solanum lycopersicum L.) зарубежной селекции различных фирм-оригинаторов, рекомендованных производителями семян как толерантные к вирусу желтой курчавости листьев томата. Все гибриды обладали комплексом хозяйственно ценных признаков и набором генов устойчивости к основным заболеваниям томата, в том числе к новому для юга России опасному патогену с максимальным потенциальным риском – вирусу желтой курчавости листьев томата (Tomato yellow leaf curl virus — TYLCV). Исследования проведены в 2017-2018 годах в лаборатории пасленовых культур ООО «НИИСОК» и в лаборатории молекулярной диагностики растений ООО «Семеновод». Всего было протестировано 34 гибрида F1 томата. Гибриды оценивали по совокупности хозяйственно ценных признаков, также проводили молекулярно-генетический анализ на наличие и аллельное состояние основных генов устойчивости: к вирусу табачной мозаики (Tm2а), фузариозному увяданию (I2), вертициллезному увяданию (Ve), к кладоспориозу (Cf9), нематодам (Mi1.2), вирусу бронзовости томата (Sw5), вирусу желтой курчавости листьев томата (Ty3a). Установлено, что все проанализированные гибриды томата с заявленной оригинаторами семян устойчивостью к вирусу желтой курчавости листьев были гетерозиготны по гену Ty3a. На основании проведенных исследований и с учетом требований рынка разработаны модели гибридов F1 томата юга России. Перспективный гибрид томата должен обладать индетерминантным типом роста с укороченными междоузлиями (4,5-5 см) а также хорошей облиственностью. Плоды томата должны быть с красной равномерной окраской без зеленого пятна у плодоножки, с плоскоокруглой или округлой формой плода и со средней массой 220-270 г. Для повышения транспортабельности томатов необходимо, чтобы плоды отличались высокой прочностью и характеризовались хорошей лежкостью. Урожайность гибрида томата должна быть более 30 кг/м2, а товарность - не менее 85%. Гибрид томата должен обладать следующим набором генов устойчивости в гетерозиготном состоянии: Ty3a, Mi1.2, Cf-9, а также в гомозиготном состоянии: Tm2a, I2, Ve. The article provides information on the study of the collection of F1 tomato hybrids (Solanum lycopersicumL.) of foreign breeding from various firms-originators recommended for cultivation in regions with a strong spread of tomato yellow leaf curl virus. All hybrids had a complex of economically valuable traits and a set of genes for resistance to the main diseases of tomato, including a new dangerous pathogen for the South of Russia with a maximum potential risk — the tomato yellow leaf curl virus (TYLCV). The studies were carried out in 2017-2018 in the Solanaceae Laboratory of LLC NIISOK and in the Molecular Diagnostics Laboratory of Plants of LLC Semenovod. A total of 34 F1 tomato hybrids were tested. The hybrids were assessed by a set of economically valuable traits. Molecular genetic analysis was also carried out for the presence and allelic state of the main resistance genes: Tomato mosaic virus (Tm2a), Fusarium wilt (I2), Werticillium wilt (Ve), Cladosporium fulvum (Cf9), Nematodes (Mi1.2), Tomato spotted wilt virus (Sw5), Tomato yellow leaf curl virus (Ty3a). It was found that all the analyzed tomato hybrids with the declared by seed originators resistance to yellow leaf curl virus were heterozygous for the Ty3a gene. Based on the conducted research and taking into account the market requirements, models of F1 tomato hybrids for protected ground for the South of Russia have been developed. A promising tomato hybrid should have an indeterminate growth type with shortened internodes (4.5-5 cm) and good foliage. Tomato fruits should have a uniform red color without green shoulders, with a flat-round or round shape of the fruit and with an average weight of 220-270 g. To increase the transportability of tomatoes, it is necessary that the fruits are highly firm and characterized by good shelf life. The yield of tomato hybrid should be more than 30 kg/m2, and marketability should be at least 85%. The tomato hybrid should have the following set of resistance genes in a heterozygous state: Ty3a, Mi1.2, Cf-9, and also in a homozygous state: Tm2a, I2, Ve.

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