Identification and genetic characterization of a novel circular single-stranded DNA virus in a human upper respiratory tract sample

Infections caused by Streptococcus suis (S. suis) pose a problem for the pig industry worldwide. Pigs often carry multiple serotypes of S. suis in the upper respiratory tract, where S. suis is frequently isolated from. The clinical diagnosis of the infection is presumptive and is generally based on clinical signs, the age of the animal and macroscopic lesions. In the laboratory, identification of S. suis is performed biochemically, and then, serotyping is performed with antisera to determine the serotype, but these tests can be inconclusive. To date, there are few studies that have documented the presence and diversity of S. suis serotypes in Mexico. In the present study, it was characterized S. suis strains from Mexican pig farms using molecular approaches; samples were first processed by PCR of the gdh gene to detect S. suis. Positive samples were then subjected to a two-step multiplex PCR (cps PCR) to detect and characterize each strain; the first step consisted of a grouping PCR and the second step consisted of a typing PCR. The serotypes detected in the pig farming areas of Mexico included 1/2, 2, 3, 5, 7, 8, 9, 17, and 23. These findings are important for the characterization of serotypes present in Mexico and for outbreak prevention.

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Characterization of antibiotic resistance and host-microbiome interactions in the human upper respiratory tract during influenza infection

Microbiome ◽

10.1186/s40168-020-00803-2 ◽

2020 ◽

Vol 8 (1) ◽

Cited By ~ 5

Author(s):

Lingdi Zhang ◽

Christian V. Forst ◽

Aubree Gordon ◽

Gabrielle Gussin ◽

Adam B. Geber ◽

...

Keyword(s):

Antibiotic Resistance ◽

Respiratory Tract ◽

Influenza Infection ◽

Upper Respiratory Tract ◽

Upper Respiratory

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Characterization of the Upper Respiratory Tract Microbiomes of Patients with Pandemic H1N1 Influenza

PLoS ONE ◽

10.1371/journal.pone.0069559 ◽

2013 ◽

Vol 8 (7) ◽

pp. e69559 ◽

Cited By ~ 38

Author(s):

Bonnie Chaban ◽

Arianne Albert ◽

Matthew G. Links ◽

Jennifer Gardy ◽

Patrick Tang ◽

...

Keyword(s):

Respiratory Tract ◽

H1n1 Influenza ◽

Pandemic H1n1 ◽

Upper Respiratory Tract ◽

Pandemic H1n1 Influenza ◽

Upper Respiratory

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Characterization of the upper respiratory tract microbiome of turkeys

10.21203/rs.3.rs-33858/v1 ◽

2020 ◽

Cited By ~ 2

Author(s):

Olimpia Kursa ◽

Grzegorz Tomczyk ◽

Anna Sawicka-Durkalec ◽

Aleksandra Giza ◽

Magdalena Słomiany-Szwarc

Keyword(s):

16S Rrna ◽

Respiratory Tract ◽

Pathogenic Bacteria ◽

Rrna Gene ◽

Upper Respiratory Tract ◽

Bacterial Phyla ◽

Sequencing Technologies ◽

Upper Respiratory ◽

Commercial Turkey

Abstract Background: The respiratory tracts of turkeys are the main route of infection therefore plays important roles in the overall health and performance of the birds. Understanding the poultry microbiome has the potential to offer better diagnosis and rational management of many poultry diseases. Characterization of microbial communities in the upper respiratory tract of turkeys could help better understand the role of pathogenic bacteria and other commensal or symbiotic microorganisms in the infection. The aim of this study was microbiome characterization of upper respiratory tracks of commercial turkeys using next-generation sequencing technologies. Results: The microbiome from samples collected from commercial turkey flocks was determined using 16S rRNA metagenomic approach. Taxonomic analysis of the microbiome was done by of the V3 and V4 regions of 16S rRNA gene (MiSeq, Illumina) amplification. The phylogenetic analysis identified the 10 bacterial phyla in turkey, the most abundant were phyla Firmicutes and Proteobacteria, accounting for >99% of all the sequences. The turkey sequences represent 144 established bacterial genera. Differences between bacterial abundances were found at the family and genus level. Several defining markers of microbiome succession were identified, including the presence of Ornithobacterium and Mycoplasma. Conclusions: Understanding the turkey’s respiratory microbiome is very important. Unique informations about microbiome representing members of the four major phyla of the respiratory tract in turkeys was assembled. These results obtained in this study supply information about turkey microbiome and can be useful in controlling, diagnosing and treating commercial turkey flocks. Our study significantly broaden the knowledge of the upper respiratory tract microbiome of turkeys.

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Streptococcus equi subsp. zooepidemicus associated with sudden death of swine in North America

10.1101/812636 ◽

2019 ◽

Author(s):

Matheus O. de Costa ◽

Brad Lage

Keyword(s):

North America ◽

Sudden Death ◽

Respiratory Tract ◽

Whole Genome ◽

Upper Respiratory Tract ◽

Genome Characterization ◽

Swine Pathogen ◽

Streptococcus Equi ◽

Upper Respiratory

AbstractHistorically described as a commensal of the swine upper-respiratory tract, Streptococcus equi subsp. zooepidemicus was only reported previously in Asia as an important swine pathogen. Here we report the isolation and whole genome characterization of Streptococcus equi subsp. zooepidemicus associated with a sudden death outbreak in pigs in North America.

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Characterization of upper respiratory tract isolates from children with recurrent tonsillitis

Research Society and Development ◽

10.33448/rsd-v10i15.23181 ◽

2021 ◽

Vol 10 (15) ◽

pp. e363101523181

Author(s):

Aline Cristine Magalhães Costa Messias ◽

Thaís Alves de Oliveira ◽

Carolina Rodrigues Andrade ◽

Raylane Pereira Gomes ◽

Célia Regina Malveste Ito ◽

...

Keyword(s):

Respiratory Tract ◽

Biofilm Formation ◽

Associated Factors ◽

Test Group ◽

Upper Respiratory Tract ◽

Recurrent Tonsillitis ◽

Antimicrobial Sensitivity ◽

Biofilm Production ◽

Upper Respiratory

To analyze the profile of airway Upper Respiratory Tract isolates from children with recurrent tonsillitis. Samples were taken using nasal cavity, oropharynx and nasopharynx swabs from30 children called the test group before tonsillectomy. Counting, isolation, identification and sequencing of the 16S rRNA, biofilm production and antimicrobial sensitivity investigation were performed. Staphylococcus aureus (S. aureus) was the only microorganism recovered in 36.6% of patients, being more present in the oropharynx and with greater resistance to erythromycin 95%, penicillin 85% and cefoxitin 85%. All isolates were formed by biofilm, 20% formed by strongly adherent biofilm. S. aureus resistant and biofilm formed, were isolated in the three studied sites, suggesting that this species contributes to recurrent tonsillitis. Impact of the study: The ability of S. aureus to acquire resistance and its other associated factors such as biofilm formation, can make this microorganism recover more easily in the microbiota of patients, taking into account the other microorganisms present there after the use of antibacterial.

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