scholarly journals Characterization of upper respiratory tract isolates from children with recurrent tonsillitis

2021 ◽  
Vol 10 (15) ◽  
pp. e363101523181
Author(s):  
Aline Cristine Magalhães Costa Messias ◽  
Thaís Alves de Oliveira ◽  
Carolina Rodrigues Andrade ◽  
Raylane Pereira Gomes ◽  
Célia Regina Malveste Ito ◽  
...  

To analyze the profile of airway Upper Respiratory Tract isolates from children with recurrent tonsillitis. Samples were taken using nasal cavity, oropharynx and nasopharynx swabs from30 children called the test group before tonsillectomy. Counting, isolation, identification and sequencing of the 16S rRNA, biofilm production and antimicrobial sensitivity investigation were performed. Staphylococcus aureus (S. aureus) was the only microorganism recovered in 36.6% of patients, being more present in the oropharynx and with greater resistance to erythromycin 95%, penicillin 85% and cefoxitin 85%. All isolates were formed by biofilm, 20% formed by strongly adherent biofilm. S. aureus resistant and biofilm formed, were isolated in the three studied sites, suggesting that this species contributes to recurrent tonsillitis. Impact of the study: The ability of S. aureus to acquire resistance and its other associated factors such as biofilm formation, can make this microorganism recover more easily in the microbiota of patients, taking into account the other microorganisms present there after the use of antibacterial.

Author(s):  
Avis Aman Nowbuth ◽  
Josh Barrie Armstrong ◽  
Thomas Eugene Cloete ◽  
Pieter Rousseau Fourie

Sanitisation has become a major component of everyday life, with emphasis on the hands and surfaces. The face remains unsanitised due to the lack of an acceptable sanitiser. The use of masks has been mandated to reduce the spread of the pathogens by covering the face, however, there remain issues with the use of personal protective equipment. The face remains a harbour for upper respiratory tract infections, with constant deposition of microbes. By reducing microbial load, the risk of both infection and severity are reduced. HOCl has proven antimicrobial and anti-inflammatory activity, including efficacy against SARS-CoV-2. A facial sanitiser, alongside hand sanitisers and masks, improves protection against SARS-CoV-2. The advantages of regular sanitising of the face and mask include reduced level of microbial contamination, risk of biofilm formation, and respiratory tract and skin infections. HOCl was reviewed as a face and mask sanitiser, concluding that it was an ideal product.


Author(s):  
Avis Aman Nowbuth ◽  
Josh Barrie Armstrong ◽  
Thomas Eugene Cloete ◽  
Pieter Rousseau Fourie

Sanitisation has become a major component of everyday life, with emphasis on the hands and surfaces. The face remains unsanitised due to the lack of an acceptable sanitiser. The use of masks has been mandated to reduce the spread of the pathogens by covering the face, however, there remain issues with the use of personal protective equipment. The face remains a harbour for upper respiratory tract infections, with constant deposition of microbes. By reducing microbial load, the risk of both infection and severity are reduced. HOCl has proven antimicrobial and anti-inflammatory activity, including efficacy against SARS-CoV-2. A facial sanitiser, alongside hand sanitisers and masks, improves protection against SARS-CoV-2. The advantages of regular sanitising of the face and mask include reduced level of microbial contamination, risk of biofilm formation, and respiratory tract and skin infections. HOCl was reviewed as a face and mask sanitiser, concluding that it was an ideal product.


2021 ◽  
Vol 12 (3) ◽  
pp. 681-693
Author(s):  
Arianna Romero Flores ◽  
Marcelo Gottschalk ◽  
Gabriela Bárcenas Morales ◽  
Víctor Quintero Ramírez ◽  
Rosario Esperanza Galván Pérez ◽  
...  

Infections caused by Streptococcus suis (S. suis) pose a problem for the pig industry worldwide. Pigs often carry multiple serotypes of S. suis in the upper respiratory tract, where S. suis is frequently isolated from. The clinical diagnosis of the infection is presumptive and is generally based on clinical signs, the age of the animal and macroscopic lesions. In the laboratory, identification of S. suis is performed biochemically, and then, serotyping is performed with antisera to determine the serotype, but these tests can be inconclusive. To date, there are few studies that have documented the presence and diversity of S. suis serotypes in Mexico. In the present study, it was characterized S. suis strains from Mexican pig farms using molecular approaches; samples were first processed by PCR of the gdh gene to detect S. suis. Positive samples were then subjected to a two-step multiplex PCR (cps PCR) to detect and characterize each strain; the first step consisted of a grouping PCR and the second step consisted of a typing PCR. The serotypes detected in the pig farming areas of Mexico included 1/2, 2, 3, 5, 7, 8, 9, 17, and 23. These findings are important for the characterization of serotypes present in Mexico and for outbreak prevention.


2018 ◽  
Vol 70 (2) ◽  
pp. 333-341
Author(s):  
C. Jaramillo ◽  
M.P. Arias Gutierrez

ABSTRACT To determine the prevalence of diseases of the upper respiratory tract and associated factors in the Colombian Creole Horse Valley Aburrá in 2015, a random sampling of 15 farms in southern Aburrá Valley under different management conditions was performed. A total of 105 CCC underwent general clinical examination, respiratory endoscopic evaluation and a detailed inspection of housing conditions and management. A low prevalence of upper respiratory tract diseases was observed, including Pharyngeal Lymphoid Hyperplasia 38.1%, Recurrent Laryngeal Neurophaty 12.38%, Dorsal Displacement of the Soft Palate 7.62%, and presence of tracheal secretions not associated with a specific pathology. A bivariate analysis showed association between tracheal secretions and frequency of training per week (OR = 9.86), duration of the training sessions (OR = 5.55) and inadequate ventilation of the stable (OR = 10.52). Association between HLR and inadequately ventilated barns was also observed (OR = 14.9). Logistic regression showed association between tracheal secretions and inadequate ventilation (OR = 7.18). We conclude that inadequate ventilation was the most important factor for the incidence of upper respiratory tract diseases in horses at the southern area of the Aburrá Valley.


Microbiome ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Lingdi Zhang ◽  
Christian V. Forst ◽  
Aubree Gordon ◽  
Gabrielle Gussin ◽  
Adam B. Geber ◽  
...  

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