Establishment and application of an indirect ELISA for porcine circovirus 3

2017 ◽  
Vol 163 (2) ◽  
pp. 479-482 ◽  
Author(s):  
Junhua Deng ◽  
Xiangdong Li ◽  
DingDing Zheng ◽  
Yanwei Wang ◽  
Lingyan Chen ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Sujiao Zhang ◽  
Dongliang Wang ◽  
Yifan Jiang ◽  
Zhoumian Li ◽  
Yawen Zou ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Indirect Elisa ◽  
Porcine Circovirus ◽  
Igg Antibodies ◽  
Porcine Circovirus 3

Molecular Characterization of Novel Porcine Circovirus 3 (PCV3) in Pig Populations in the North of Vietnam

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nguyen Van Giap ◽  
Chung Hee Chun ◽  
Huynh Thi My Le ◽  
Cao Thi Bich Phuong ◽  
Vu Thi Ngoc ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Circovirus ◽  
The North ◽  
Porcine Circovirus 3

Development and application of an indirect ELISA for the detection of antibody to porcine circovirus 4 in pigs

2021 ◽  
Author(s):  
Zhengmin Lian ◽  
Jinbiao Liu ◽  
Panrao Liu ◽  
Zhenbang Zhu ◽  
Xiaohui Yao ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Porcine Circovirus

Enzymatic recombinase amplification coupled with CRISPR-Cas12a for ultrasensitive, rapid, and specific Porcine circovirus 3 detection

2021 ◽  
Vol 59 ◽  
pp. 101763
Author(s):  
Wuyin Zhang ◽  
Liang Xu ◽  
Qi Liu ◽  
Yingli Cao ◽  
Kankan Yang ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Porcine Circovirus 3

Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

2018 ◽  
Vol 253 ◽  
pp. 26-30 ◽  
Author(s):  
Yu-Ri Park ◽  
Hye-Ryung Kim ◽  
Seong-Hee Kim ◽  
Kyoung-Ki Lee ◽  
Young S. Lyoo ◽  
...  
Keyword(s):  
Visual Detection ◽  
Isothermal Amplification ◽  
Porcine Circovirus ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Porcine Circovirus 3

scholarly journals Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

2020 ◽  
Vol 72 (5) ◽  
pp. 1731-1736
Author(s):  
A.C.M. Cruz ◽  
I.L.F. Rodrigues ◽  
A.E. Souza ◽  
F.B. Knackfuss ◽  
R.L. Silveira ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Circovirus ◽  
Southeastern Brazil ◽  
Clinical Aspects ◽  
Statistical Association ◽  
Serum Samples ◽  
Clinical Presentations ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Swine Serum

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

scholarly journals Detection and genetic characterization of porcine circovirus 3 from aborted fetuses and pigs with respiratory disease in Korea

2018 ◽  
Vol 19 (5) ◽  
pp. 721 ◽  
Author(s):  
Seong-Hee Kim ◽  
Ji-Young Park ◽  
Ji-Youl Jung ◽  
Ha-Young Kim ◽  
Yu-Ri Park ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Genetic Characterization ◽  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Development of a SYBR green I-based duplex real-time PCR assay for detection of pseudorabies virus and porcine circovirus 3

2020 ◽  
Vol 53 ◽  
pp. 101593 ◽  
Author(s):  
Run-Bo Tian ◽  
Yue Jin ◽  
Tong Xu ◽  
Yu Zhao ◽  
Zhen-Ya Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pseudorabies Virus ◽  
Sybr Green ◽  
Sybr Green I ◽  
Porcine Circovirus ◽  
Pcr Assay ◽  
Porcine Circovirus 3

scholarly journals Sensitive detection of porcine circovirus 3 by droplet digital PCR

2019 ◽  
Vol 31 (4) ◽  
pp. 604-607
Author(s):  
Yuqi Liu ◽  
Hecheng Meng ◽  
Lei Shi ◽  
Lili Li
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Porcine Circovirus ◽  
Analytical Sensitivity ◽  
Swine Industry ◽  
Potential Threat ◽  
Emerging Virus ◽  
Clinical Detection ◽  
Porcine Circovirus 3 ◽  
High Degree

Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/μL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection.

scholarly journals Frequency of Detection and Phylogenetic Analysis of Porcine circovirus 3 (PCV-3) in Healthy Primiparous and Multiparous Sows and Their Mummified Fetuses and Stillborn

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 533 ◽  
Author(s):  
Viviane Saporiti ◽  
Susanna Martorell ◽  
Taís F. Cruz ◽  
Francini Klaumann ◽  
Florencia Correa-Fiz ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Circovirus ◽  
Reproductive Parameters ◽  
Reproductive Disorders ◽  
Time Points ◽  
Lack Of Information ◽  
Complete Genomes ◽  
Reproductive Disease ◽  
Porcine Circovirus 3 ◽  
Intrauterine Infections

Porcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders.

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