Detection and analysis of mycovirus-related RNA viruses from grape powdery mildew fungus Erysiphe necator

2018 ◽  
Vol 163 (4) ◽  
pp. 1019-1030 ◽  
Author(s):  
B. Pandey ◽  
R. A. Naidu ◽  
G. G. Grove
Keyword(s):  
Powdery Mildew ◽  
Rna Viruses ◽  
Powdery Mildew Fungus ◽  
Erysiphe Necator ◽  
Grape Powdery Mildew

Genetic Variation and Population Structure of the Grape Powdery Mildew Fungus, Erysiphe necator, in Southern France

2005 ◽  
Vol 113 (4) ◽  
pp. 407-416 ◽  
Author(s):  
Jean-Pierre Péros ◽  
Claire Troulet ◽  
Mikaël Guerriero ◽  
Corinne Michel-Romiti ◽  
Jean-Loup Notteghem
Keyword(s):  
Population Structure ◽  
Genetic Variation ◽  
Powdery Mildew ◽  
Powdery Mildew Fungus ◽  
Erysiphe Necator ◽  
Southern France ◽  
Grape Powdery Mildew

scholarly journals Adaptive genomic structural variation in the grape powdery mildew pathogen, Erysiphe necator

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 1081 ◽  
Author(s):  
Laura Jones ◽  
Summaira Riaz ◽  
Abraham Morales-Cruz ◽  
Katherine CH Amrine ◽  
Brianna McGuire ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Structural Variation ◽  
Erysiphe Necator ◽  
Genomic Structural Variation ◽  
Powdery Mildew Pathogen ◽  
Grape Powdery Mildew

scholarly journals Differential Gene Expression During Conidiation in the Grape Powdery Mildew Pathogen, Erysiphe necator

2011 ◽  
Vol 101 (7) ◽  
pp. 839-846 ◽  
Author(s):  
Laura Wakefield ◽  
David M. Gadoury ◽  
Robert C. Seem ◽  
Michael G. Milgroom ◽  
Qi Sun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Powdery Mildew ◽  
Differential Gene Expression ◽  
Polymorphism Analysis ◽  
Erysiphe Necator ◽  
Powdery Mildews ◽  
Differential Gene ◽  
Powdery Mildew Pathogen ◽  
Fungal Genes ◽  
Grape Powdery Mildew

Asexual sporulation (conidiation) is coordinately regulated in the grape powdery mildew pathogen Erysiphe necator but nothing is known about its genetic regulation. We hypothesized that genes required for conidiation in other fungi would be upregulated at conidiophore initiation or full conidiation (relative to preconidiation vegetative growth and development of mature ascocarps), and that the obligate biotrophic lifestyle of E. necator would necessitate some novel gene regulation. cDNA amplified fragment length polymorphism analysis with 45 selective primer combinations produced ≈1,600 transcript-derived fragments (TDFs), of which 620 (39%) showed differential expression. TDF sequences were annotated using BLAST analysis of GenBank and of a reference transcriptome for E. necator developed by 454-FLX pyrosequencing of a normalized cDNA library. One-fourth of the differentially expressed, annotated sequences had similarity to fungal genes of unknown function. The remaining genes had annotated function in metabolism, signaling, transcription, transport, and protein fate. As expected, a portion of orthologs known in other fungi to be involved in developmental regulation was upregulated immediately prior to or during conidiation; particularly noteworthy were several genes associated with the light-dependent VeA regulatory system, G-protein signaling (Pth11 and a kelch repeat), and nuclear transport (importin-β and Ran). This work represents the first investigation into differential gene expression during morphogenesis in E. necator and identifies candidate genes and hypotheses for characterization in powdery mildews. Our results indicate that, although control of conidiation in powdery mildews may share some basic elements with established systems, there are significant points of divergence as well, perhaps due, in part, to the obligate biotrophic lifestyle of powdery mildews.

Specific Isolation of RNA from the Grape Powdery Mildew Pathogen Erysiphe necator, an Epiphytic, Obligate Parasite

2010 ◽  
Vol 158 (1) ◽  
pp. 69-71 ◽  
Author(s):  
Lance Cadle-Davidson ◽  
Laura Wakefield ◽  
Robert C. Seem ◽  
David M. Gadoury
Keyword(s):  
Powdery Mildew ◽  
Erysiphe Necator ◽  
Obligate Parasite ◽  
Powdery Mildew Pathogen ◽  
Grape Powdery Mildew

scholarly journals Evaluation of Quinoxyfen Resistance of Erysiphe necator (Grape Powdery Mildew) in a Single Virginia Vineyard

Plant Disease ◽  
2018 ◽  
Vol 102 (12) ◽  
pp. 2586-2591 ◽  
Author(s):  
Xuewen Feng ◽  
Mizuho Nita ◽  
Anton B. Baudoin
Keyword(s):  
United States ◽  
Powdery Mildew ◽  
Disease Control ◽  
Low Dose ◽  
Disease Incidence ◽  
The United States ◽  
Field Trials ◽  
Erysiphe Necator ◽  
Late Season ◽  
Grape Powdery Mildew

The protectant fungicide quinoxyfen has been used against grape powdery mildew (Erysiphe necator) in the United States since 2003. In 2013, isolates of grape powdery mildew with reduced quinoxyfen sensitivity (here designated as quinoxyfen lab resistance or QLR) were detected in a single vineyard in western Virginia, USA. Field trials were conducted in 2014, 2015, and 2016 at the affected vineyard to determine to what extent quinoxyfen might still contribute to disease control. Powdery mildew control by quinoxyfen was similar to, or only slightly less than, that provided by myclobutanil and boscalid in all three years. In 2016, early- versus late-season applications of quinoxyfen were compared to test the hypothesis that early-season applications were more effective, but differences were small. A treatment with two early quinoxyfen applications, at bloom and 2 weeks later, followed by a myclobutanil-boscalid plus a low dose of sulfur rotation provided slightly better control of foliar disease incidence than treatments containing four quinoxyfen applications or two midseason or two late quinoxyfen applications supplemented by myclobutanil and boscalid applications; severity differences were small and nonsignificant. Metrafenone and benzovindiflupyr generally provided excellent powdery mildew control. The frequency of QLR in vines not treated with quinoxyfen slowly declined from 65% in 2014 to 46% in 2016. Further research is needed to explain how, despite this QLR frequency, quinoxyfen applied to grapes in the field was still able to effectively control powdery mildew.

Interruption and Reduction of Erysiphe necator Chasmothecia Development Utilizing Fungicidal Oil

2018 ◽  
Vol 19 (2) ◽  
pp. 153-155
Author(s):  
L. D. Thiessen ◽  
T. M. Neill ◽  
W. F. Mahaffee
Keyword(s):  
Powdery Mildew ◽  
Disease Incidence ◽  
Growing Season ◽  
Management Option ◽  
Erysiphe Necator ◽  
Grape Powdery Mildew

Grape powdery mildew epidemics, caused by Erysiphe necator, are initiated by the release and subsequent germination of ascospores from mature chasmothecia. Interrupting the development of chasmothecia prior to overwintering may reduce or prevent the overwintering of E. necator in vineyards, thereby reducing initial disease incidence observed the following growing season. At the end of the grape growing season in 2014 and 2015, one application of Organic JMS Stylet Oil (Vero Beach, FL) at a rate of 10 ml/liter was applied on four treatment dates using an air-assisted backpack sprayer onto Chardonnay grapevines within a vineyard. Leaves were collected weekly and chasmothecia enumerated from the first observation of chasmothecia primordia development until the onset of rains (approximately 5 weeks). In 2014, all stylet oil treatment plots developed significantly fewer chasmothecia than nontreated control plots in 2014 (P = 0.04), and there were no differences in treatment date observed. No treatment differences were observed in 2015. Because chasmothecia were still produced despite stylet oil treatment, a single post-véraison stylet oil application to interrupt chasmothecia development would not be an economical management option for reduction of grape powdery mildew.

A mechanistic model simulating ascosporic infections by Erysiphe necator, the powdery mildew fungus of grapevine

2010 ◽  
Vol 60 (3) ◽  
pp. 522-531 ◽  
Author(s):  
T. Caffi ◽  
V. Rossi ◽  
S. E. Legler ◽  
R. Bugiani
Keyword(s):  
Powdery Mildew ◽  
Mechanistic Model ◽  
Powdery Mildew Fungus ◽  
Erysiphe Necator

A degree-day model to initiate fungicide spray programs for management of grape powdery mildew [Erysiphe necator]

2009 ◽  
Vol 31 (2) ◽  
pp. 186-194 ◽  
Author(s):  
O. Carisse ◽  
R. Bacon ◽  
A. Lefebvre ◽  
K. Lessard
Keyword(s):  
Powdery Mildew ◽  
Erysiphe Necator ◽  
Degree Day ◽  
Grape Powdery Mildew
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