Expression of goose parvovirus VP1 capsid protein by a baculovirus expression system and establishment of fluorescent antibody test to diagnose goose parvovirus infection

1999 ◽  
Vol 144 (8) ◽  
pp. 1639-1645 ◽  
Author(s):  
K. Takehara ◽  
T. Nakata ◽  
K. Takizawa ◽  
C. K. Limn ◽  
K. Mutoh ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Fluorescent Antibody ◽  
Expression System ◽  
Antibody Test ◽  
Baculovirus Expression System ◽  
Baculovirus Expression ◽  
Goose Parvovirus ◽  
Vp1 Capsid Protein

scholarly journals Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses

2000 ◽  
Vol 38 (9) ◽  
pp. 3492-3494 ◽  
Author(s):  
Shinichi Kobayashi ◽  
Kenji Sakae ◽  
Yasumoto Suzuki ◽  
Kuniko Shinozaki ◽  
Mineyuki Okada ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Capsid Protein ◽  
Immunosorbent Assay ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Capsid ◽  
Specific Detection ◽  
Japanese Strain ◽  
Baculovirus Expression

The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.

scholarly journals Expression of 1B capsid protein of Foot-and-mouth disease virus (FMDV) using baculovirus expression system and its validation in detecting SAT 2- specific antisera

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8946
Author(s):  
Wael Elmenofy ◽  
Ismail Mohamed ◽  
Lamiaa El-Gaied ◽  
Reda Salem ◽  
Gamal Osman ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Disease Virus ◽  
Culture Filtrate ◽  
Insect Cells ◽  
Expression System ◽  
Foot And Mouth Disease ◽  
Baculovirus Expression System ◽  
Specific Antibodies ◽  
Baculovirus Expression ◽  
Mouth Disease Virus

Foot-and-mouth disease virus (FMDV) is one of the most devastating animal viruses that affect livestock worldwide. The 1B capsid of FMDV has been widely used to detect and confirm the infection. In the present study, the sequence coding for 1B subunit of FMDV capsid was expressed in insect cells using the baculovirus expression system under the polyhedrin (polh) promoter. The expression of 1B capsid protein was validated in the culture filtrate of insect cells using SDS-PAGE and western blotting. The culture filtrate containing recombinant 1B capsid (r1B) was used as a coated antigen in an indirect enzyme-linked immunosorbent assay (ELISA). The antigenicity and specificity of r1B against SAT 2 serotype-specific antibodies were assessed. Our results revealed that a protein concentration as low as 25 ng could detect SAT 2-specific antibodies in ELISA. The results highlight the application of insect cells developed r1B protein in the detection of FMDV. Further studies are required to determine the ability of r1B to detect other FMDV serotypes.

scholarly journals Expression of functional G protein beta gamma dimers of defined subunit composition using a baculovirus expression system.

1992 ◽  
Vol 267 (19) ◽  
pp. 13123-13126 ◽  
Author(s):  
S.G. Graber ◽  
R.A. Figler ◽  
V.K. Kalman-Maltese ◽  
J.D. Robishaw ◽  
J.C. Garrison
Keyword(s):  
G Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Subunit Composition ◽  
Baculovirus Expression
Sign in / Sign up

Export Citation Format

Share Document