Cloning and molecular characterization of a putative bZIP transcription factor VvbZIP23 from Vitis vinifera

PROTOPLASMA ◽  
2012 ◽  
Vol 250 (1) ◽  
pp. 333-345 ◽  
Author(s):  
Himanshu Tak ◽  
Minal Mhatre
Keyword(s):  
Transcription Factor ◽  
Vitis Vinifera ◽  
Molecular Characterization ◽  
Bzip Transcription Factor

Molecular characterization of a stress-response bZIP transcription factor in banana

2012 ◽  
Vol 113 (2) ◽  
pp. 173-187 ◽  
Author(s):  
Sen He ◽  
Wei Shan ◽  
Jian-fei Kuang ◽  
Hui Xie ◽  
Yun-yi Xiao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Response ◽  
Molecular Characterization ◽  
Bzip Transcription Factor

scholarly journals Isolation and molecular characterization of a Lotus japonicus R2R3-MYB subgroup 7 transcription factor gene

2017 ◽  
Vol 34 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Satoru Kunihiro ◽  
Daigo Tanabe ◽  
Yuiko Niwa ◽  
Keisuke Kitamura ◽  
Jun Abe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Lotus Japonicus ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
R2r3 Myb

Molecular characterization of Japanese indigenous grape cultivar ‘Koshu’ (Vitis vinifera) leaf and berry skin during grape development

2009 ◽  
Vol 3 (3) ◽  
pp. 225-241 ◽  
Author(s):  
Hironori Kobayashi ◽  
Keiko Fujita ◽  
Shunji Suzuki ◽  
Tsutomu Takayanagi
Keyword(s):  
Vitis Vinifera ◽  
Molecular Characterization ◽  
Berry Skin ◽  
Grape Cultivar

scholarly journals Molecular Characterization of Saccharomyces cerevisiae TFIID

2002 ◽  
Vol 22 (16) ◽  
pp. 6000-6013 ◽  
Author(s):  
Steven L. Sanders ◽  
Krassimira A. Garbett ◽  
P. Anthony Weil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Molecular Mass ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Gel Filtration ◽  
Calculated Molecular Mass ◽  
General Transcription Factor

ABSTRACT We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription in vitro and displays TATA box binding activity that is functionally distinct from that of TBP. Analyses of the stoichiometry of TFIID subunits indicated that several TAFs are present at more than 1 copy per TFIID complex. This conclusion was further supported by coimmunoprecipitation experiments with a systematic family of (pseudo)diploid yeast strains that expressed epitope-tagged and untagged alleles of the genes encoding TFIID subunits. Based on these data, we calculated a native molecular mass for monomeric TFIID. Purified TFIID behaved in a fashion consistent with this calculated molecular mass in both gel filtration and rate-zonal sedimentation experiments. Quite surprisingly, although the TAF subunits of TFIID cofractionated as a single complex, TBP did not comigrate with the TAFs during either gel filtration chromatography or rate-zonal sedimentation, suggesting that TBP has the ability to dynamically associate with the TFIID TAFs. The results of direct biochemical exchange experiments confirmed this hypothesis. Together, our results represent a concise molecular characterization of the general transcription factor TFIID from S. cerevisiae.

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