Medium optimization for production of gamma-aminobutyric acid by Lactobacillus brevis NCL912

Amino Acids ◽  
2009 ◽  
Vol 38 (5) ◽  
pp. 1439-1445 ◽  
Author(s):  
Haixing Li ◽  
Ting Qiu ◽  
Dandan Gao ◽  
Yusheng Cao
Keyword(s):  
Medium Optimization ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Lactobacillus Brevis Ncl912

scholarly journals Optimization of Gamma Aminobutyric Acid Production Using High Pressure Processing (HPP) by Lactobacillus brevis PML1

2022 ◽  
Vol 2022 ◽  
pp. 1-9
Author(s):  
Atefe Ghafurian Nasab ◽  
Sayed Ali Mortazavi ◽  
Farideh Tabatabaei Yazdi ◽  
Mahboobe Sarabi Jamab
Keyword(s):  
High Performance ◽  
Morphological Changes ◽  
Control Sample ◽  
Bioactive Compound ◽  
Lactobacillus Brevis ◽  
High Pressure Processing ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Viability Analysis ◽  
Layer Chromatography

In the present research, the production potential of gamma aminobutyric acid (GABA) using Lactobacillus brevis PML1 was investigated. In addition, the microorganism viability was examined in MAN, ROGOSA, and SHARPE (MRS) after undergoing high hydrostatic pressure at 100, 200, and 300 MPa for 5, 10, and 15 min. Response surface methodology (RSM) was applied to optimize the production conditions of GABA as well as the bacteria viability. Analysis of variance (ANOVA) indicated that both the independent variables (pressure and time) significantly influenced the dependent ones (GABA and bacteria viability) ( P < 0.05 ). The optimum extraction conditions to maximize the production of GABA included the pressure of 300 MPa and the time of 15 min. The amount of the compound was quantified using thin-layer chromatography (TLC) and spectrophotometry. For the process optimization, a central composite design (CCD) was created using Design Expert with 5 replications at the center point, whereby the highest content of GABA was obtained to be 397.73 ppm which was confirmed by high performance liquid chromatography (HPLC). Moreover, scanning electron microscopy (SEM) was utilized to observe the morphological changes in the microorganism. The results revealed that not only did have Lactobacillus brevis PML1 the potential for the production of GABA under conventional conditions (control sample) but also the content of this bioactive compound could be elevated by optimizing the production parameters.

scholarly journals Gamma-aminobutyric acid-producing lactobacilli positively affect metabolism and depressive-like behaviour in a mouse model of metabolic syndrome

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
E. Patterson ◽  
P. M. Ryan ◽  
N. Wiley ◽  
I. Carafa ◽  
E. Sherwin ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Mouse Model ◽  
Plasma Cholesterol ◽  
Forced Swim Test ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Metabolic Dysfunction ◽  
Gamma Aminobutyric Acid ◽  
Gaba Production ◽  
Glucose Challenge

Abstract Metabolic and neuroactive metabolite production represents one of the mechanisms through which the gut microbiota can impact health. One such metabolite, gamma-aminobutyric acid (GABA), can modulate glucose homeostasis and alter behavioural patterns in the host. We previously demonstrated that oral administration of GABA-producing Lactobacillus brevis DPC6108 has the potential to increase levels of circulating insulin in healthy rats. Therefore, the objective of this study was to assess the efficacy of endogenous microbial GABA production in improving metabolic and behavioural outcomes in a mouse model of metabolic dysfunction. Diet-induced obese and metabolically dysfunctional mice received one of two GABA-producing strains, L. brevis DPC6108 or L. brevis DSM32386, daily for 12 weeks. After 8 and 10 weeks of intervention, the behavioural and metabolic profiles of the mice were respectively assessed. Intervention with both L. brevis strains attenuated several abnormalities associated with metabolic dysfunction, causing a reduction in the accumulation of mesenteric adipose tissue, increased insulin secretion following glucose challenge, improved plasma cholesterol clearance and reduced despair-like behaviour and basal corticosterone production during the forced swim test. Taken together, this exploratory dataset indicates that intervention with GABA-producing lactobacilli has the potential to improve metabolic and depressive- like behavioural abnormalities associated with metabolic syndrome in mice.

scholarly journals Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice

2022 ◽  
Vol 11 (1) ◽  
pp. 165-176 ◽  
Author(s):  
Amro Abdelazez ◽  
Heba Abdelmotaal ◽  
Smith Etareri Evivie ◽  
Maha Bikheet ◽  
Rokayya Sami ◽  
...  
Keyword(s):  
Gastric Juice ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Diabetic Mice

scholarly journals Gamma-aminobutyric acid fermentation with date residue by a lactic acid bacterium, Lactobacillus brevis

2018 ◽  
Vol 125 (3) ◽  
pp. 316-319 ◽  
Author(s):  
Momoko Hasegawa ◽  
Daisuke Yamane ◽  
Kouichi Funato ◽  
Atsushi Yoshida ◽  
Yoshihiro Sambongi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Acid Fermentation
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