Prevalence and quantitative analysis of Epstein–Barr virus DNA and Porphyromonas gingivalis associated with Japanese chronic periodontitis patients

Abstract Background Initial periodontal therapy (IPT) is cornerstone of periodontal therapy and the first step to control of periodontal risk factors. Scaling and root planing are used to treat root surface irregularities and remove virulent factors caused by periodontal pathogens. This procedure also incorporated into periodontal surgery. To elucidate the effects of IPT on prevalence of Epstein-Barr virus (EBV) DNA and Porphyromonas gingivalis, we used subgingival plaque samples from chronic periodontitis (CP) patients. Methods Seventeen CP patients were recruited and measured periodontal clinical parameters such as probing pocket depth (PD) and bleeding on probing (BOP), and subgingival plaque samples were collected from two periodontal sites with PD of <3 mm (healthy sites: HS) or >5 mm (periodontitis sites: PS) at first visit and after IPT. Plaque samples were subjected to a real-time PCR to detect EBV DNA and P. gingivalis. Results EBV DNA and P. gingivalis were detected 9 (52.9%) and 14 (82.3%) in the subgingival samples from HS, and 13 (76.5%) and 14 (82.3%) in the PS at first visit. After IPT, number of detections of EBV DNA and P. gingivalis were decreased to 5 (29.4%) and 13 (76.5%) in the HS, and 9 (52.9%) and 10 (58.8%) in the PS. Significant improvements in PD and BOP were observed after IPT in PS. Coexistence of EBV DNA and P. gingivalis in the subgingival samples from PS at first visit (12; 70.6%) were significantly decreased after IPT (6; 35.3%). Conclusion These results suggest that the IPT was effective in improvement of clinical parameters such as PD and BOP and reducing the coexistence of EBV and P. gingivalis in the subgingival plaque from PS. However, IPT could not eradicate the EBV and P. gingivalis. Further research would be necessary for improving the periodontal treatment strategy.

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Effects of initial periodontal therapy on the prevalence of Epstein-Barr virus DNA and Porphyromonas gingivalis in Japanese chronic periodontitis patients

10.21203/rs.2.16315/v1 ◽

2019 ◽

Author(s):

Ayako Kato ◽

Yorinobu Ikeda ◽

Kenichi Imai ◽

Yorimasa Ogata

Keyword(s):

Porphyromonas Gingivalis ◽

Chronic Periodontitis ◽

Epstein Barr Virus ◽

Periodontal Therapy ◽

Subgingival Plaque ◽

Clinical Parameters ◽

Periodontal Pathogens ◽

Barr Virus ◽

Ebv Dna ◽

Epstein Barr

Abstract Background Initial periodontal therapy (IPT) is cornerstone of periodontal therapy and the first step to control of periodontal risk factors. Scaling and root planing are used to treat root surface irregularities and remove virulent factors caused by periodontal pathogens. This procedure also incorporated into periodontal surgery. To elucidate the effects of IPT on prevalence of Epstein-Barr virus (EBV) DNA and Porphyromonas gingivalis , we used subgingival plaque samples from chronic periodontitis (CP) patients.Methods Seventeen CP patients were recruited and determined measured periodontal status clinical parameters such as by probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP) and an X-ray examination, and subgingival plaque samples were collected from two periodontal sites with PD of < 3 mm (healthy sites: HS) or > 5 mm (periodontitis sites: PS) at first visit and after IPT. Plaque samples were subjected to a real-time PCR to detect EBV DNA and P. gingivalis.Results EBV DNA and P. gingivalis were detected 9 (52.9%) and 14 (82.3%) sites within the subgingival samples from HS, and 13 (76.5%) and 14 (82.3%) sites within the PS at first visit. After IPT, number of detections of EBV DNA and P. gingivalis were decreased to 5 (29.4%) and 13 (76.5%) sites within the subgingival samples from HS, and 9 (52.9%) and 10 (58.8%) sites within the PS. Significant improvements in PD and BOP were observed after IPT in PS. Coexistence of EBV DNA and P. gingivalis in the subgingival samples from PS at first visit (12 sites; 70.6%) were significantly decreased after IPT (6 sites; 35.3%).Conclusion These results suggest that the IPT was effective in improvement of clinical parameters such as PD and BOP and reducing the coexistence of EBV and P. gingivalis in the subgingival plaque from PS. However, IPT could not eradicate the EBV and P. gingivalis . Further research would be necessary for improving the periodontal treatment strategy.

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Effects of Initial Periodontal Therapy on the Prevalence of Epstein-Barr Virus DNA and Porphyromonas gingivalis in Japanese Chronic Periodontitis Patients

International Journal of Oral-Medical Sciences ◽

10.5466/ijoms.19.30 ◽

2020 ◽

Vol 19 (1) ◽

pp. 30-37

Author(s):

Ayako Kato ◽

Yorinobu Ikeda ◽

Kenichi Imai ◽

Yorimasa Ogata

Keyword(s):

Porphyromonas Gingivalis ◽

Chronic Periodontitis ◽

Epstein Barr Virus ◽

Periodontal Therapy ◽

Barr Virus ◽

Epstein Barr

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Quantitative analysis of circulating cell-free Epstein-Barr virus (EBV) DNA levels in patients with EBV-associated lymphoid malignancies

British Journal of Haematology ◽

10.1046/j.1365-2141.2000.02344.x ◽

2000 ◽

Vol 111 (1) ◽

pp. 239-246 ◽

Cited By ~ 72

Author(s):

Kenny I. K. Lei ◽

Lisa Y.S. Chan ◽

Wing Y. Chan ◽

Philip J. Johnson ◽

Y. M. Dennis Lo

Keyword(s):

Quantitative Analysis ◽

Epstein Barr Virus ◽

Barr Virus ◽

Lymphoid Malignancies ◽

Ebv Dna ◽

Epstein Barr

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Real-time polymerase chain reaction quantification of Epstein-Barr virus in chronic periodontitis patients

Journal of Periodontal Research ◽

10.1111/j.1600-0765.2005.00796.x ◽

2005 ◽

Vol 40 (4) ◽

pp. 294-298 ◽

Cited By ~ 18

Author(s):

Antonis Konstantinidis ◽

Dimitra Sakellari ◽

Anna Papa ◽

Antonis Antoniadis

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Chronic Periodontitis ◽

Epstein Barr Virus ◽

Chain Reaction ◽

Barr Virus ◽

Polymerase Chain ◽

Epstein Barr

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How Does Epstein–Barr Virus Contribute to Chronic Periodontitis?

International Journal of Molecular Sciences ◽

10.3390/ijms21061940 ◽

2020 ◽

Vol 21 (6) ◽

pp. 1940 ◽

Cited By ~ 2

Author(s):

Kenichi Imai ◽

Yorimasa Ogata

Keyword(s):

Diabetes Mellitus ◽

Chronic Periodontitis ◽

Epstein Barr Virus ◽

Systemic Diseases ◽

Periodontopathic Bacteria ◽

Barr Virus ◽

The World ◽

Latently Infected ◽

Plaque Control ◽

Epstein Barr

Chronic periodontitis is spreading worldwide and mutually interacts with systemic diseases like diabetes mellitus. Although periodontopathic bacteria are inevitable pathogens in their onset and progression, many cases are not ascribable to the virulence of these bacteria because the effect of plaque control is limited. In contrast, Epstein–Barr virus (EBV) in the periodontium has been correlated with chronic periodontitis and has recently been considered as a promising pathogenic candidate for this disease. However, several important questions have yet to be addressed. For instance, although EBV latently infects more than 90% of individuals over the world, why do patients with chronic periodontitis exclusively harbor progeny EBV in the oral cavity? In addition, how does latently infected or reactivated EBV in the periodontium relate to the onset or progression of chronic periodontitis? Finally, is periodontitis incurable because EBV is the pathogen for chronic periodontitis? In this review, we attempt to answer these questions by reporting the current understanding of molecular relations and mechanisms between periodontopathic bacteria and EBV reactivation in the context of how this relationship may pertain to the etiology of chronic periodontitis.

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