Biological effects of low-level laser irradiation (LLLI) on stem cells from human exfoliated deciduous teeth (SHED)

2019 ◽  
Vol 24 (1) ◽  
pp. 167-180 ◽  
Author(s):  
M. Paschalidou ◽  
E. Athanasiadou ◽  
K. Arapostathis ◽  
N. Kotsanos ◽  
P. T. Koidis ◽  
...  
2017 ◽  
Vol 33 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Lélia Batista de Souza ◽  
Carlos Augusto Galvão Barboza

AIP Advances ◽  
2016 ◽  
Vol 6 (4) ◽  
pp. 045018 ◽  
Author(s):  
Hongli Chen ◽  
Hong Wang ◽  
Yingxin Li ◽  
Weichao Liu ◽  
Chao Wang ◽  
...  

2019 ◽  
Vol 34 (9) ◽  
pp. 1917-1924 ◽  
Author(s):  
Luciano Aparecido Almeida-Junior ◽  
Nádia Carolina Teixeira Marques ◽  
Mariel Tavares de Oliveira Prado ◽  
Thais Marchini Oliveira ◽  
Vivien Thiemy Sakai

2017 ◽  
Vol 15 (3) ◽  
pp. 334-338 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Carlos Augusto Galvão Barboza

ABSTRACT Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry. Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups. Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.


2016 ◽  
Vol 24 (4) ◽  
pp. 332-337 ◽  
Author(s):  
Ana Paula FERNANDES ◽  
Marina de Azevedo JUNQUEIRA ◽  
Nádia Carolina Teixeira MARQUES ◽  
Maria Aparecida Andrade Moreira MACHADO ◽  
Carlos Ferreira SANTOS ◽  
...  

2015 ◽  
Vol 30 (9) ◽  
pp. 2259-2264 ◽  
Author(s):  
Ivana Maria Zaccara ◽  
Fernanda Ginani ◽  
Haroldo Gurgel Mota-Filho ◽  
Águida Cristina Gomes Henriques ◽  
Carlos Augusto Galvão Barboza

Sign in / Sign up

Export Citation Format

Share Document