scholarly journals Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

AIP Advances ◽  
2016 ◽  
Vol 6 (4) ◽  
pp. 045018 ◽  
Author(s):  
Hongli Chen ◽  
Hong Wang ◽  
Yingxin Li ◽  
Weichao Liu ◽  
Chao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Laser Irradiation ◽  
Umbilical Cord ◽  
Biological Effects ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation

Biological effects of low-level laser irradiation (LLLI) on stem cells from human exfoliated deciduous teeth (SHED)

2019 ◽  
Vol 24 (1) ◽  
pp. 167-180 ◽  
Author(s):  
M. Paschalidou ◽  
E. Athanasiadou ◽  
K. Arapostathis ◽  
N. Kotsanos ◽  
P. T. Koidis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Laser Irradiation ◽  
Biological Effects ◽  
Deciduous Teeth ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation ◽  
Human Exfoliated Deciduous Teeth

Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix

2005 ◽  
Vol 20 (3-4) ◽  
pp. 138-146 ◽  
Author(s):  
Liat Abramovitch-Gottlib ◽  
Talia Gross ◽  
Doron Naveh ◽  
Shimona Geresh ◽  
Salman Rosenwaks ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Laser Irradiation ◽  
Three Dimensional ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation

scholarly journals Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells

2014 ◽  
Vol 12 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Carlos Augusto Galvão Barboza ◽  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Águida Cristina Gomes Henriques ◽  
Roseana de Almeida Freitas
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Laser Irradiation ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Nuclear Alterations ◽  
Low Level Laser Irradiation

Objective : To evaluate the effect of low-level laser irradiation on the proliferation and possible nuclear morphological changes of mouse mesenchymal stem cells. Methods : Mesenchymal stem cells derived from bone marrow and adipose tissue were submitted to two applications (T0 and T48 hours) of low-level laser irradiation (660nm; doses of 0.5 and 1.0J/cm2). The trypan blue assay was used to evaluate cell viability, and growth curves were used to analyze proliferation at zero, 24, 48, and 72 hours. Nuclear alterations were evaluated by staining with DAPI (4’-6-diamidino-2-phenylindole) at 72 hours. Results : Bone marrow-derived mesenchymal stem cells responded to laser therapy in a dose-dependent manner. Higher cell growth was observed when the cells were irradiated with a dose of 1.0J/cm2, especially after 24 hours (p<0.01). Adipose-derived mesenchymal stem cells responded better to a dose of 1.0J/cm2, but higher cell proliferation was observed after 48 hours (p<0.05) and 72 hours (p<0.01). Neither nuclear alterations nor a significant change in cell viability was detected in the studied groups. Conclusion : Low-level laser irradiation stimulated the proliferation of mouse mesenchymal stem cells without causing nuclear alterations. The biostimulation of mesenchymal stem cells using laser therapy might be an important tool for regenerative therapy and tissue engineering.

scholarly journals Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

2017 ◽  
Vol 15 (3) ◽  
pp. 334-338 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Carlos Augusto Galvão Barboza
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Laser Irradiation ◽  
Adipose Derived Stem Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
Low Level Laser Irradiation

ABSTRACT Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry. Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups. Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.

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