Low-level laser irradiation induces in vitro proliferation of stem cells from human exfoliated deciduous teeth

2017 ◽  
Vol 33 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Lélia Batista de Souza ◽  
Carlos Augusto Galvão Barboza
Keyword(s):  
Stem Cells ◽  
Laser Irradiation ◽  
Deciduous Teeth ◽  
In Vitro Proliferation ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation ◽  
Human Exfoliated Deciduous Teeth

Biological effects of low-level laser irradiation (LLLI) on stem cells from human exfoliated deciduous teeth (SHED)

2019 ◽  
Vol 24 (1) ◽  
pp. 167-180 ◽  
Author(s):  
M. Paschalidou ◽  
E. Athanasiadou ◽  
K. Arapostathis ◽  
N. Kotsanos ◽  
P. T. Koidis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Laser Irradiation ◽  
Biological Effects ◽  
Deciduous Teeth ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation ◽  
Human Exfoliated Deciduous Teeth

In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation

2011 ◽  
Author(s):  
Nora Bloise ◽  
Enrica Saino ◽  
Francesca Bragheri ◽  
Paolo Minzioni ◽  
Ilaria Cristiani ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Human Osteoblast ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Vitro Analysis ◽  
Low Level Laser Irradiation ◽  
In Vitro Analysis

Effect of Low-Level Laser Irradiation on In Vitro Function of Pancreatic Islets

2009 ◽  
Vol 41 (10) ◽  
pp. 4313-4315 ◽  
Author(s):  
S. Irani ◽  
S.S. Mohseni Salehi Monfared ◽  
M. Akbari-Kamrani ◽  
S.N. Ostad ◽  
M. Abdollahi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Laser Irradiation ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation

Effect of single and multiple doses of low-level laser therapy on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED)

2019 ◽  
Vol 34 (9) ◽  
pp. 1917-1924 ◽  
Author(s):  
Luciano Aparecido Almeida-Junior ◽  
Nádia Carolina Teixeira Marques ◽  
Mariel Tavares de Oliveira Prado ◽  
Thais Marchini Oliveira ◽  
Vivien Thiemy Sakai
Keyword(s):  
Stem Cells ◽  
Laser Therapy ◽  
Low Level Laser Therapy ◽  
Deciduous Teeth ◽  
Low Level ◽  
Low Level Laser ◽  
Single And Multiple Doses ◽  
Multiple Doses ◽  
Level Laser ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

2017 ◽  
Vol 15 (3) ◽  
pp. 334-338 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Carlos Augusto Galvão Barboza
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Laser Irradiation ◽  
Adipose Derived Stem Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
Low Level Laser Irradiation

ABSTRACT Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry. Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups. Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.

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