Local (but not systemic) photobiomodulation treatment reduces mast cell degranulation, eicosanoids, and Th2 cytokines in an experimental model of allergic rhinitis

2021 ◽  
Author(s):  
Adriana Schapochnik ◽  
Simone Klein ◽  
Robson Brochetti ◽  
Paula Tatiane Alonso ◽  
Amílcar Sabino Damazo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cell ◽  
Experimental Model ◽  
Mast Cell Degranulation ◽  
Th2 Cytokines

Transcutaneous systemic photobiomodulation reduced lung inflammation in experimental model of asthma by altering the mast cell degranulation and interleukin 10 level

2021 ◽  
Author(s):  
Paula Tatiane Alonso ◽  
Adriana Schapochnik ◽  
Simone Klein ◽  
Robson Brochetti ◽  
Amílcar Sabino Damazo ◽  
...  
Keyword(s):  
Mast Cell ◽  
Experimental Model ◽  
Lung Inflammation ◽  
Interleukin 10 ◽  
Mast Cell Degranulation

A laboratory demonstration for learning about mast cell degranulation.

1999 ◽  
Vol 276 (6) ◽  
pp. S19
Author(s):  
J Corado ◽  
A Eblen-Zajjur
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Experimental Model ◽  
Low Cost ◽  
Mast Cell Degranulation ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

A simple experimental model of cell degranulation was implemented that exposed mast cells obtained from Sprague-Dawley rats to saponin. The model is flexible, asy, and low cost, is not very time-consuming to run, and needs a minimum of laboratory resources. It has been used for the last three years in our undergraduate medical physiology courses and has replaced the classic utilization of slides and drawings.

scholarly journals Increased Mast Cell Degranulation within Thalamus in Early Pre-lesion Stages of an Experimental Model of Wernickeʼs Encephalopathy

1999 ◽  
Vol 58 (7) ◽  
pp. 773-783 ◽  
Author(s):  
Melissa Ferguson ◽  
Andrea M. Dalve-Endres ◽  
Robert Carter McRee ◽  
Philip J. Langlais
Keyword(s):  
Mast Cell ◽  
Experimental Model ◽  
Mast Cell Degranulation

scholarly journals Design of a heterotetravalent synthetic allergen that reflects epitope heterogeneity and IgE antibody variability to study mast cell degranulation

2012 ◽  
Vol 449 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Michael W. Handlogten ◽  
Tanyel Kiziltepe ◽  
Basar Bilgicer
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Experimental Model ◽  
Experimental System ◽  
Mast Cell Degranulation ◽  
Integrative Approach ◽  
Ige Antibody

The present paper describes the design of a HtTA (heterotetravalent allergen) as a multi-component experimental system that enables an integrative approach to study mast cell degranulation. The HtTA design allows presentation of two distinct haptens, each with a valency of 2, thereby better reflecting the complexity of natural allergens by displaying epitope heterogeneity and IgE antibody variability. Using the HtTA design, synthetic allergens HtTA-1 and HtTA-2 were synthesized to model a combination of epitope/IgE affinities. HtTA-1 presented DNP (2,4-dinitrophenyl) and dansyl haptens (Kd=22 and 54 nM for IgEDNP and IgEdansyl respectively) and HtTA-2 presented dansyl and the weak-affinity DNP-Pro (DNP-proline) haptens (Kd=550 nM for IgEDNP). Both HtTAs effectively induced degranulation when mast cells were primed with both IgEDNP and IgEdansyl antibodies. Interestingly tetravalent DNP-Pro or bivalent dansyl were insufficient in stimulating a degranulation response, illustrating the significance of valency, affinity and synergy in allergen–IgE interactions. Importantly, maximum degranulation with both HtTA-1 and HtTA-2 was observed when only 50% of the mast cell-bound IgEs were hapten-specific (25% IgEdansyl and 25% IgEDNP). Taken together, results of the present study establish the HtTA system as a physiologically relevant experimental model and demonstrates its utility in elucidating critical mechanisms of mast cell degranulation.

Effects of point application on celiac mast cell degranulation in mice with allergic rhinitis: An experimental study

2010 ◽  
Vol 8 (2) ◽  
pp. 75-79 ◽  
Author(s):  
Jie Chen ◽  
Xin-sheng Lai ◽  
Chun-zhi Tang ◽  
Li-lei He ◽  
Bing-xu Jin
Keyword(s):  
Experimental Study ◽  
Allergic Rhinitis ◽  
Mast Cell ◽  
Mast Cell Degranulation

Mast cell ultrastructure in the inferior turbinate and stroma of nasal polyps

1997 ◽  
Vol 111 (4) ◽  
pp. 340-345 ◽  
Author(s):  
Adrian Drake-Lee ◽  
Jacqueline Price
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cell ◽  
Mast Cells ◽  
Nasal Polyps ◽  
Inferior Turbinate ◽  
Cell Ultrastructure ◽  
Mast Cell Degranulation ◽  
Perennial Allergic Rhinitis ◽  
Ultrastructural Examination ◽  
Essentially Normal

AbstractFourteen unselected adult patients with nasal polyps had ultrastructural examination of mast cells from matching biopsies of the polyp and inferior turbinate. Between three and 10 blocks were examined for each patient in both tissues and every mast cell that had a nucleus was photographed for study. Fifty-three mast cells were found within the stroma of nasal polyps and 54 in the submucosa of the inferior turbinate biopsies. The number of granules ranged between 13 and 167 (mean 60) for polyps and 18 and 148 (mean 61) in the inferior turbinate. The mast cells appeared essentially normal in the inferior turbinate of four patients. The degree of degranulation of the mast cells was calculated as in previous studies and then averaged for both the polyp and the inferior turbinate of each patient. There was greater degranulation in the nasal polyp compared to inferior turbinate (p= 0.03). These results were compared with mast cell degranulation found in the normal nose and in the inferior turbinate of patients with perennial allergic rhinitis which we previously published. The inferior turbinates in these patients were more degranulated than the normal nose (p= 0.0001) but were similar to that found in patients with perennial allergic rhinitis. This suggested that some degree of degranulation may occur throughout the nose in two thirds of the patients with nasal polyps which supports the theory that mast cell reactions are not limited to the polyps in a proportion of patients.

scholarly journals The Anti-Allergic Rhinitis Effect of Traditional Chinese Medicine of Shenqi by Regulating Mast Cell Degranulation and Th1/Th2 Cytokine Balance

Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 504 ◽  
Author(s):  
Yang-Yang Shao ◽  
Yi-Ming Zhou ◽  
Min Hu ◽  
Jin-Ze Li ◽  
Cheng-Juan Chen ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cell ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Mast Cell Degranulation ◽  
Th2 Cytokine ◽  
Cytokine Balance

Mode of Mast Cell Degranulation

1971 ◽  
Vol 33 (3) ◽  
pp. 223-228
Author(s):  
Shojiro MORIYASU
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation
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