Transcutaneous systemic photobiomodulation reduced lung inflammation in experimental model of asthma by altering the mast cell degranulation and interleukin 10 level

2021 ◽  
Author(s):  
Paula Tatiane Alonso ◽  
Adriana Schapochnik ◽  
Simone Klein ◽  
Robson Brochetti ◽  
Amílcar Sabino Damazo ◽  
...  
Keyword(s):  
Mast Cell ◽  
Experimental Model ◽  
Lung Inflammation ◽  
Interleukin 10 ◽  
Mast Cell Degranulation

Local (but not systemic) photobiomodulation treatment reduces mast cell degranulation, eicosanoids, and Th2 cytokines in an experimental model of allergic rhinitis

2021 ◽  
Author(s):  
Adriana Schapochnik ◽  
Simone Klein ◽  
Robson Brochetti ◽  
Paula Tatiane Alonso ◽  
Amílcar Sabino Damazo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cell ◽  
Experimental Model ◽  
Mast Cell Degranulation ◽  
Th2 Cytokines

A laboratory demonstration for learning about mast cell degranulation.

1999 ◽  
Vol 276 (6) ◽  
pp. S19
Author(s):  
J Corado ◽  
A Eblen-Zajjur
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Experimental Model ◽  
Low Cost ◽  
Mast Cell Degranulation ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

A simple experimental model of cell degranulation was implemented that exposed mast cells obtained from Sprague-Dawley rats to saponin. The model is flexible, asy, and low cost, is not very time-consuming to run, and needs a minimum of laboratory resources. It has been used for the last three years in our undergraduate medical physiology courses and has replaced the classic utilization of slides and drawings.

scholarly journals Increased Mast Cell Degranulation within Thalamus in Early Pre-lesion Stages of an Experimental Model of Wernickeʼs Encephalopathy

1999 ◽  
Vol 58 (7) ◽  
pp. 773-783 ◽  
Author(s):  
Melissa Ferguson ◽  
Andrea M. Dalve-Endres ◽  
Robert Carter McRee ◽  
Philip J. Langlais
Keyword(s):  
Mast Cell ◽  
Experimental Model ◽  
Mast Cell Degranulation

scholarly journals Design of a heterotetravalent synthetic allergen that reflects epitope heterogeneity and IgE antibody variability to study mast cell degranulation

2012 ◽  
Vol 449 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Michael W. Handlogten ◽  
Tanyel Kiziltepe ◽  
Basar Bilgicer
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Experimental Model ◽  
Experimental System ◽  
Mast Cell Degranulation ◽  
Integrative Approach ◽  
Ige Antibody

The present paper describes the design of a HtTA (heterotetravalent allergen) as a multi-component experimental system that enables an integrative approach to study mast cell degranulation. The HtTA design allows presentation of two distinct haptens, each with a valency of 2, thereby better reflecting the complexity of natural allergens by displaying epitope heterogeneity and IgE antibody variability. Using the HtTA design, synthetic allergens HtTA-1 and HtTA-2 were synthesized to model a combination of epitope/IgE affinities. HtTA-1 presented DNP (2,4-dinitrophenyl) and dansyl haptens (Kd=22 and 54 nM for IgEDNP and IgEdansyl respectively) and HtTA-2 presented dansyl and the weak-affinity DNP-Pro (DNP-proline) haptens (Kd=550 nM for IgEDNP). Both HtTAs effectively induced degranulation when mast cells were primed with both IgEDNP and IgEdansyl antibodies. Interestingly tetravalent DNP-Pro or bivalent dansyl were insufficient in stimulating a degranulation response, illustrating the significance of valency, affinity and synergy in allergen–IgE interactions. Importantly, maximum degranulation with both HtTA-1 and HtTA-2 was observed when only 50% of the mast cell-bound IgEs were hapten-specific (25% IgEdansyl and 25% IgEDNP). Taken together, results of the present study establish the HtTA system as a physiologically relevant experimental model and demonstrates its utility in elucidating critical mechanisms of mast cell degranulation.

Mode of Mast Cell Degranulation

1971 ◽  
Vol 33 (3) ◽  
pp. 223-228
Author(s):  
Shojiro MORIYASU
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation

scholarly journals Transcriptional regulation of the mucosal mast cell-specific protease gene, MMCP-2, by interleukin 10 and interleukin 3.

1992 ◽  
Vol 267 (12) ◽  
pp. 8473-8477 ◽  
Author(s):  
N Ghildyal ◽  
H.P. McNeil ◽  
M.F. Gurish ◽  
K.F. Austen ◽  
R.L. Stevens
Keyword(s):  
Mast Cell ◽  
Transcriptional Regulation ◽  
Interleukin 10 ◽  
Mucosal Mast Cell ◽  
Protease Gene ◽  
Interleukin 3 ◽  
Specific Protease

scholarly journals Platelet and Erythrocyte Extravasation across Inflamed Corneal Venules Depend on CD18, Neutrophils and Mast Cell Degranulation

2021 ◽  
Vol 22 (14) ◽  
pp. 7360
Author(s):  
Angie De La Cruz ◽  
Aubrey Hargrave ◽  
Sri Magadi ◽  
Justin A. Courson ◽  
Paul T. Landry ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Types ◽  
Mast Cell Degranulation ◽  
Wild Type ◽  
Delayed Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Abrasion ◽  
Low Levels ◽  
Endothelial Pores

Platelet extravasation during inflammation is under-appreciated. In wild-type (WT) mice, a central corneal epithelial abrasion initiates neutrophil (PMN) and platelet extravasation from peripheral limbal venules. The same injury in mice expressing low levels of the β2-integrin, CD18 (CD18hypo mice) shows reduced platelet extravasation with PMN extravasation apparently unaffected. To better define the role of CD18 on platelet extravasation, we focused on two relevant cell types expressing CD18: PMNs and mast cells. Following corneal abrasion in WT mice, we observed not only extravasated PMNs and platelets but also extravasated erythrocytes (RBCs). Ultrastructural observations of engorged limbal venules showed platelets and RBCs passing through endothelial pores. In contrast, injured CD18hypo mice showed significantly less venule engorgement and markedly reduced platelet and RBC extravasation; mast cell degranulation was also reduced compared to WT mice. Corneal abrasion in mast cell-deficient (KitW-sh/W-sh) mice showed less venule engorgement, delayed PMN extravasation, reduced platelet and RBC extravasation and delayed wound healing compared to WT mice. Finally, antibody-induced depletion of circulating PMNs prior to corneal abrasion reduced mast cell degranulation, venule engorgement, and extravasation of PMNs, platelets, and RBCs. In summary, in the injured cornea, platelet and RBC extravasation depends on CD18, PMNs, and mast cell degranulation.

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