Lindane removal in contaminated soil by defined microbial consortia and evaluation of its effectiveness by bioassays and cytotoxicity studies

The biodegradation abilities of 10 dibenzothiophene degrading microbial consortia isolated from contaminated soil were investigated. 5 highly efficient dibenzothiophene degrading bacterial strains were obtained from the consortium LKY10 by screening on LB-agar plates.The bacterial strain LKY10-5 reduced more than 90% of dibenzothiophene with 40 mg•L-1concentration, and had higher degradation efficiency than enriched bacterial consortia in 7 days of cultivation. According to species identification and phylogenetic analysis, strain LKY10-1 and LKY10-3 belonged to Actinobacteria and could be included in Rhodococcus and Cellulosimicrobium genus, LKY10-5 and LKY10-6 belonged to Proteobacteria and could be included in Pseudomonas and Devosia genus, and LKY10-13 could be included in Lysinibacillus genus and belonged to Firmicutes.

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Biodegradation potential assessment of microbial consortia isolated from a diesel-contaminated soil

Water Science & Technology ◽

10.2166/wst.2000.0541 ◽

2000 ◽

Vol 42 (5-6) ◽

pp. 403-406 ◽

Cited By ~ 3

Author(s):

J. Milcic-Terzic ◽

Y. Lopez-Vidal ◽

M.M. Vrvic ◽

S. Saval

Keyword(s):

Contaminated Soil ◽

Microbial Consortia ◽

Catabolic Genes ◽

Xyle Gene ◽

Pcr Techniques ◽

Naphthalene Biodegradation

Diesel, toluene and naphthalene-degrading microbial consortia were isolated from a diesel-contaminated soil. The presence of catabolic genes, xylE and ndoB responsible for toluene/xylene and naphthalene biodegradation, respectively, were screened by PCR techniques in all microbial consortia. The diesel-consortium possessed both catabolic genes, the toluene-consortium only the xylE gene, while the naphthalene-consortium possessed only the ndoB gene. On the basis of these results, it was concluded that contaminated soil has indigenous microbes with a high natural potential for biodegradation.

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Bioremediation of PAH-Contaminated Soil via In-Vessel Composting

Water Science & Technology ◽

10.2166/wst.1992.0729 ◽

1992 ◽

Vol 26 (9-11) ◽

pp. 2331-2334 ◽

Cited By ~ 2

Author(s):

A. O. Adenuga ◽

J. H. Johnson ◽

J. N. Cannon ◽

L. Wan

Keyword(s):

Moisture Content ◽

Contaminated Soil ◽

High Performance ◽

Dry Weight ◽

Total Radioactivity ◽

Weight Basis ◽

Microbial Consortia ◽

Liquid Chromatograph ◽

Polycyclic Aromatic ◽

Pyrene Concentration

This study is being conducted to investigate the biodegradation of polycyclic aromatic hydrocarbons (PAHs) present in contaminated soil using composting technology. Pyrene, a four-ring aromatic hydrocarbon, was chosen as a model PAH compound for the study. Preliminary investigations were carried out to assess the feasibility of soil composting by monitoring (a) carbon to nitrogen (C/N) ratio, (b) microbial consortia diversity, (c) coefficient of permeability and (d) moisture content. The soil was augmented with composted sludge, which was used as a bulking agent and nutrient supplement, in quantities ranging from 0-50% dry weight basis. Tests were conducted in both batch and continuous composters. In the batch composter, forty grams of soil/sludge mixtures in different proportions were spiked with 13.5 mg/kg (dry weight basis) of unlabeled pyrene. The batch composters were aerated and placed in a constant temperature water bath for a period of 21 days. Samples were soxhelet extracted and analyzed on the High Performance Liquid Chromatograph to determine the reduction in pyrene concentration. Analysis of these samples is in progress. In the continuous composter, the soil/sludge mixtures are spiked with both labeled and un-labclcd pyrene to obtain a total radioactivity of 13.5 µci. This process is being monitored for temperature, moisture content, volatile solids and microbial population. Mineralization of pyrene is assessed by quantifying 14CO2 produced.

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Bioremediation of a polyaromatic hydrocarbon contaminated soil by native soil microbiota and bioaugmentation with isolated microbial consortia

Bioresource Technology ◽

10.1016/j.biortech.2009.03.079 ◽

2009 ◽

Vol 100 (20) ◽

pp. 4669-4675 ◽

Cited By ~ 90

Author(s):

Ísis Serrano Silva ◽

Eder da Costa dos Santos ◽

Cristiano Ragagnin de Menezes ◽

Andréia Fonseca de Faria ◽

Elisangela Franciscon ◽

...

Keyword(s):

Contaminated Soil ◽

Microbial Consortia ◽

Polyaromatic Hydrocarbon ◽

Soil Microbiota ◽

Native Soil

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Detection of catabolic genes in indigenous microbial consortia isolated from a diesel-contaminated soil

Bioresource Technology ◽

10.1016/s0960-8524(00)00156-5 ◽

2001 ◽

Vol 78 (1) ◽

pp. 47-54 ◽

Cited By ~ 19

Author(s):

J Milcic-Terzic

Keyword(s):

Contaminated Soil ◽

Microbial Consortia ◽

Catabolic Genes

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Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

HKIE Transactions ◽

10.33430/v26n2thie-2018-0041 ◽

2019 ◽

Vol 26 (2) ◽

pp. 63-71

Author(s):

Ling Leng ◽

Ying Wang ◽

Peixian Yang ◽

Takashi Narihiro ◽

Masaru Konishi Nobu ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Microbial Consortia ◽

Chain Elongation ◽

Desulfovibrio Vulgaris ◽

Rrna Gene ◽

Selective Enrichment ◽

Microbial Network ◽

Cost Efficient ◽

Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

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