Biodegradation potential assessment of microbial consortia isolated from a diesel-contaminated soil

2000 ◽  
Vol 42 (5-6) ◽  
pp. 403-406 ◽  
Author(s):  
J. Milcic-Terzic ◽  
Y. Lopez-Vidal ◽  
M.M. Vrvic ◽  
S. Saval
Keyword(s):  
Contaminated Soil ◽  
Microbial Consortia ◽  
Catabolic Genes ◽  
Xyle Gene ◽  
Pcr Techniques ◽  
Naphthalene Biodegradation

Diesel, toluene and naphthalene-degrading microbial consortia were isolated from a diesel-contaminated soil. The presence of catabolic genes, xylE and ndoB responsible for toluene/xylene and naphthalene biodegradation, respectively, were screened by PCR techniques in all microbial consortia. The diesel-consortium possessed both catabolic genes, the toluene-consortium only the xylE gene, while the naphthalene-consortium possessed only the ndoB gene. On the basis of these results, it was concluded that contaminated soil has indigenous microbes with a high natural potential for biodegradation.

Impact of nanoparticles on transcriptional regulation of catabolic genes of petroleum hydrocarbon-degrading bacteria in contaminated soil microcosms

2018 ◽  
Vol 59 (2) ◽  
pp. 166-180
Author(s):  
Wael S. El-Sayed ◽  
Yasser Elbahloul ◽  
Mohamed E. Saad ◽  
Ahmed M. Hanafy ◽  
Abdelrahman H. Hegazi ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Soil Microcosms ◽  
Catabolic Genes ◽  
Degrading Bacteria

Effect of process parameters on the bioremediation of diesel contaminated soil by mixed microbial consortia

2016 ◽  
Vol 113 ◽  
pp. 375-385 ◽  
Author(s):  
Abu Lahel ◽  
Abaynesh Belay Fanta ◽  
Natalia Sergienko ◽  
Manisha Shakya ◽  
M. Estefanía López ◽  
...  
Keyword(s):  
Process Parameters ◽  
Contaminated Soil ◽  
Microbial Consortia

Isolation and Genetic Identification of Dibenzothiophene Degrading Bacteria from Contaminated Soil

2012 ◽  
Vol 610-613 ◽  
pp. 292-295 ◽  
Author(s):  
Lin Li ◽  
Chao Cheng Zhao ◽  
Qi You Liu ◽  
Yun Bo Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Contaminated Soil ◽  
Bacterial Strain ◽  
Degradation Efficiency ◽  
Genetic Identification ◽  
Microbial Consortia ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The biodegradation abilities of 10 dibenzothiophene degrading microbial consortia isolated from contaminated soil were investigated. 5 highly efficient dibenzothiophene degrading bacterial strains were obtained from the consortium LKY10 by screening on LB-agar plates.The bacterial strain LKY10-5 reduced more than 90% of dibenzothiophene with 40 mg•L-1concentration, and had higher degradation efficiency than enriched bacterial consortia in 7 days of cultivation. According to species identification and phylogenetic analysis, strain LKY10-1 and LKY10-3 belonged to Actinobacteria and could be included in Rhodococcus and Cellulosimicrobium genus, LKY10-5 and LKY10-6 belonged to Proteobacteria and could be included in Pseudomonas and Devosia genus, and LKY10-13 could be included in Lysinibacillus genus and belonged to Firmicutes.

scholarly journals Molecular Diversity of Plasmids Bearing Genes That Encode Toluene and Xylene Metabolism in Pseudomonas Strains Isolated from Different Contaminated Sites in Belarus

2000 ◽  
Vol 66 (7) ◽  
pp. 2842-2852 ◽  
Author(s):  
Vladimir S. Sentchilo ◽  
Alexander N. Perebituk ◽  
Alexander J. B. Zehnder ◽  
Jan Roelof van der Meer
Keyword(s):  
Dna Sequencing ◽  
Contaminated Soil ◽  
Molecular Diversity ◽  
Restriction Analysis ◽  
Soil Samples ◽  
Contaminated Sites ◽  
Diversity Analysis ◽  
Common Ancestry ◽  
Catabolic Genes ◽  
Genetic Mechanisms

ABSTRACT Twenty different Pseudomonas strains utilizingm-toluate were isolated from oil-contaminated soil samples near Minsk, Belarus. Seventeen of these isolates carried plasmids ranging in size from 78 to about 200 kb (assigned pSVS plasmids) and encoding the meta cleavage pathway for toluene metabolism. Most plasmids were conjugative but of unknown incompatibility groups, except for one, which belonged to the IncP9 group. The organization of the genes for toluene catabolism was determined by restriction analysis and hybridization with xyl gene probes of pWW0. The majority of the plasmids carried xyl-type genes highly homologous to those of pWW53 and organized in a similar manner (M. T. Gallegos, P. A. Williams, and J. L. Ramos, J. Bacteriol. 179:5024–5029, 1997), with two distinguishable metapathway operons, one upper pathway operon, and threexylS-homologous regions. All of these plasmids also possessed large areas of homologous DNA outside the catabolic genes, suggesting a common ancestry. Two other pSVS plasmids carried only onemeta pathway operon, one upper pathway operon, and one copy each of xylS and xylR. The backbones of these two plasmids differed greatly from those of the others. Whereas these parts of the plasmids, carrying the xyl genes, were mostly conserved between plasmids of each group, the noncatabolic parts had undergone intensive DNA rearrangements. DNA sequencing of specific regions near and within the xylTE and xylAgenes of the pSVS plasmids confirmed the strong homologies to thexyl genes of pWW53 and pWW0. However, several recombinations were discovered within the upper pathway operons of the pSVS plasmids and pWW0. The main genetic mechanisms which are thought to have resulted in the present-day configuration of thexyl operons are discussed in light of the diversity analysis carried out on the pSVS plasmids.

Bioremediation of PAH-Contaminated Soil via In-Vessel Composting

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2331-2334 ◽  
Author(s):  
A. O. Adenuga ◽  
J. H. Johnson ◽  
J. N. Cannon ◽  
L. Wan
Keyword(s):  
Moisture Content ◽  
Contaminated Soil ◽  
High Performance ◽  
Dry Weight ◽  
Total Radioactivity ◽  
Weight Basis ◽  
Microbial Consortia ◽  
Liquid Chromatograph ◽  
Polycyclic Aromatic ◽  
Pyrene Concentration

This study is being conducted to investigate the biodegradation of polycyclic aromatic hydrocarbons (PAHs) present in contaminated soil using composting technology. Pyrene, a four-ring aromatic hydrocarbon, was chosen as a model PAH compound for the study. Preliminary investigations were carried out to assess the feasibility of soil composting by monitoring (a) carbon to nitrogen (C/N) ratio, (b) microbial consortia diversity, (c) coefficient of permeability and (d) moisture content. The soil was augmented with composted sludge, which was used as a bulking agent and nutrient supplement, in quantities ranging from 0-50% dry weight basis. Tests were conducted in both batch and continuous composters. In the batch composter, forty grams of soil/sludge mixtures in different proportions were spiked with 13.5 mg/kg (dry weight basis) of unlabeled pyrene. The batch composters were aerated and placed in a constant temperature water bath for a period of 21 days. Samples were soxhelet extracted and analyzed on the High Performance Liquid Chromatograph to determine the reduction in pyrene concentration. Analysis of these samples is in progress. In the continuous composter, the soil/sludge mixtures are spiked with both labeled and un-labclcd pyrene to obtain a total radioactivity of 13.5 µci. This process is being monitored for temperature, moisture content, volatile solids and microbial population. Mineralization of pyrene is assessed by quantifying 14CO2 produced.

scholarly journals Bioremediation of a polyaromatic hydrocarbon contaminated soil by native soil microbiota and bioaugmentation with isolated microbial consortia

2009 ◽  
Vol 100 (20) ◽  
pp. 4669-4675 ◽  
Author(s):  
Ísis Serrano Silva ◽  
Eder da Costa dos Santos ◽  
Cristiano Ragagnin de Menezes ◽  
Andréia Fonseca de Faria ◽  
Elisangela Franciscon ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Microbial Consortia ◽  
Polyaromatic Hydrocarbon ◽  
Soil Microbiota ◽  
Native Soil

Isolation and characterization of naphthalene-catabolic genes and plasmids from oil-contaminated soil by using two cultivation-independent approaches

2007 ◽  
Vol 74 (2) ◽  
pp. 501-510 ◽  
Author(s):  
Akira Ono ◽  
Ryo Miyazaki ◽  
Masahiro Sota ◽  
Yoshiyuki Ohtsubo ◽  
Yuji Nagata ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Isolation And Characterization ◽  
Catabolic Genes
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