Rapid Isolation of DNA from Fresh and Preserved Fish Scales for Polymerase Chain Reaction

2001 ◽  
Vol 3 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Gen Hua Yue ◽  
Laszlo Orban
Keyword(s):  
Polymerase Chain Reaction ◽  
Fish Scales ◽  
Chain Reaction ◽  
Rapid Isolation ◽  
Polymerase Chain

A Simple and Affordable Method of DNA Extraction from Fish Scales for Polymerase Chain Reaction

2012 ◽  
Vol 51 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
Yanhe Li ◽  
Yasmeen Gul ◽  
Zexia Gao ◽  
Wei Luo ◽  
Weimin Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Fish Scales ◽  
Chain Reaction ◽  
Polymerase Chain

Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction

Genomics ◽  
1990 ◽  
Vol 6 (3) ◽  
pp. 475-481 ◽  
Author(s):  
Susan A. Ledbetter ◽  
David L. Nelson ◽  
Stephen T. Warren ◽  
David H. Ledbetter
Keyword(s):  
Polymerase Chain Reaction ◽  
Repetitive Sequence ◽  
Dna Probes ◽  
Specific Chromosome ◽  
Chain Reaction ◽  
Rapid Isolation ◽  
Polymerase Chain ◽  
Chromosome Regions

Rapid Isolation of Flanking Genomic DNA Using Biotin-RAGE, a Variation of Single-Sided Polymerase Chain Reaction

1992 ◽  
Vol 11 (10) ◽  
pp. 791-797 ◽  
Author(s):  
BRIAN T. BLOOMQUIST ◽  
RICHARD C. JOHNSON ◽  
RICHARD E. MAINS
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Chain Reaction ◽  
Rapid Isolation ◽  
Polymerase Chain

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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