Rapid Isolation of Cell-Type-Specific Protein Tyrosine Kinases by Degenerate Polymerase Chain Reaction Combined with Differential Hybridization Technique

1995 ◽  
Vol 214 (1) ◽  
pp. 60-68 ◽  
Author(s):  
S.J. Kim ◽  
H. Sasaki ◽  
A. Takahashi ◽  
M. Katoh ◽  
T. Kakizoe ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Tyrosine Kinases ◽  
Specific Protein ◽  
Protein Tyrosine Kinases ◽  
Hybridization Technique ◽  
Cell Type ◽  
Chain Reaction ◽  
Rapid Isolation ◽  
Cell Type Specific ◽  
Polymerase Chain

Detection of Platelet-Specific Protein mRNAs in Different Megakaryoblasts Using the Reverse Transcriptase Polymerase Chain Reaction

1992 ◽  
Vol 7 (5-6) ◽  
pp. 505-510 ◽  
Author(s):  
Mutsumi Yasunaga ◽  
Ryukichi Ryo ◽  
Wataru Sugano ◽  
Nobuo Yamaguchi
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Specific Protein ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Resveratrol reduced the detrimental effects of malondialdehyde on human endothelial cells

2021 ◽  
Vol 13 (2) ◽  
pp. 131-140
Author(s):  
Mehdi Hassanpour ◽  
Çıgır Biray Avci ◽  
Reza Rahbarghazi ◽  
Aysa Rezabakhsh ◽  
Alireza Nourazarian ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Endothelial Cells ◽  
Surface Plasmon Resonance ◽  
Chromatin Remodeling ◽  
Tyrosine Kinases ◽  
Receptor Tyrosine Kinases ◽  
Pcr Array ◽  
Human Endothelial Cells ◽  
Chain Reaction ◽  
Polymerase Chain

Introduction: According to the statistics, vascular injury occurs during the onset of diabetic changes after the production of several byproducts. Many authorities have focused to find an alternative therapy for diabetic patients. In this study, we investigated the therapeutic effects of natural polyphenol like resveratrol on human endothelial cells exposed to malondialdehyde for 48 hours. Methods: Human Umbilical Vein Endothelial Cells were randomly classified into four groups;control, malondialdehyde (2.5 mM), resveratrol (100 μM), and cells received the combined regime for 48 hours. Cell viability was determined by 3-(4, 5-dimethyl thiazol-2-yl) 2, 5-diphenyl-tetrazoliumbromide (MTT) assay. Griess reaction was performed to measure the content of Nitric oxide (NO).Apoptosis was studied by using real-time polymerase chain reaction (RT-PCR) and western blotting assays. Levels of receptor tyrosine kinases like VEGFR-1, -2, Tie-1, and -2 were analyzed by enzyme-linked immunosorbent assay(ELISA). The affinity of resveratrol and malondialdehyde to serum albumin was measured by Surface Plasmon Resonance Assay. Any changes in chromatin remodeling were detected by PCR array analysis. Results: Resveratrol reduced cytotoxicity and NO content inside cells induced by malondialdehyde(MDA) (P < 0.05). Endothelial cell apoptosis was decreased by the reduction of pro-apoptotic factor Bax and increase of Bcl-2 following the incubation with resveratrol (P < 0.05). MDA-induced receptor tyrosine kinases increase was inhibited by resveratrol and reached near-to-normal levels (P < 0.05).Surface Plasmon Resonance revealed a higher affinity of resveratrol to albumin compared to the malondialdehyde-albumin complex. Polymerase chain reaction (PCR) array revealed the potency of resveratrol in chromatin remodeling following the treatment with malondialdehyde (P < 0.05). Conclusion: Based on our findings, resveratrol has the potential to decrease diabetic vascular injury induced by lipid byproducts such as MDA.

Molecular Pathology of Soft Tissue and Bone Tumors

1999 ◽  
Vol 123 (12) ◽  
pp. 1246-1259
Author(s):  
Andrzej Slominski ◽  
Jacobo Wortsman ◽  
Andrew Carlson ◽  
Martin Mihm ◽  
Brian Nickoloff ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factors ◽  
Soft Tissue ◽  
Reverse Transcription ◽  
Intracellular Signaling ◽  
Bone Tumors ◽  
Cellular Proliferation ◽  
Hybridization Technique ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Objective.—To present recent concepts on the molecular pathogenesis of tumors of soft tissue and bone, and on the use of molecular genetic methods, including their significance as diagnostic markers and prognostic indicators. Data Sources and Study Selection.—Reports on tumors of bone and/or soft tissue published in the English language literature and observations made using specimens available at the Departments of Pathology at Albany Medical College and Loyola University Medical Center. Data Extraction and Synthesis.—Studies on bone and soft tissue tumors containing chromosomal or genetic evaluation were selected for further analysis. Specific chromosomal abnormalities, such as numerical aberrations or translocations with production of fusion genes, were classified according to the tumor of origin. Data were also collected on mutations in tumor suppressor genes, genes coding for growth factors or their receptors, and genes coding for tyrosine kinases. Also noted were mutations of uncertain significance, for which the pathogenic connection between tumor production and mutated gene function is still unclear. Conclusions.—In general, the mutations reported interfere with the action of peptide growth factors coordinating mesenchyme proliferation and differentiation, although membrane-bound receptors expressing the intracellular signaling modifier, tyrosine kinase activity, have also been involved. Functional types of genes most commonly affected include tumor suppressors, oncogenes, and nuclear transcription factors. Thus, the mutations involved in the pathogenesis of soft tissue and bone tumors have affected multiple genes. Moreover, aberrant fusion gene products may be formed in tumoral tissue and may then act as transcription regulators stimulating cellular proliferation. Cytogenetic studies help at the clinical level by demonstrating aneuploidy and increased ploidy, which may correlate with malignant behavior. Diagnostic tumor-specific chromosomal translocations may be detected with Southern hybridization analysis, polymerase chain reaction, reverse-transcription polymerase chain reaction, or with the fluorescence in situ hybridization technique. Notably, early metastatic disease may be detectable in blood specimens using polymerase chain reaction or reverse-transcription polymerase chain reaction techniques.

Enhanced Detection of HIV-1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique

Vox Sanguinis ◽  
1991 ◽  
Vol 61 (1) ◽  
pp. 24-29 ◽  
Author(s):  
S.M. Bruisten ◽  
M.H.G.M. Koppelmann ◽  
C.L. van der Poel ◽  
J.G. Huisman
Keyword(s):  
Polymerase Chain Reaction ◽  
Hybridization Technique ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Hiv 1
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