Influence of nitrogen source and pH value on undesired poly(γ-glutamic acid) formation of a protease producing Bacillus licheniformis strain

2015 ◽  
Vol 42 (9) ◽  
pp. 1203-1215 ◽  
Author(s):  
Lena Meissner ◽  
Kira Kauffmann ◽  
Timo Wengeler ◽  
Hitoshi Mitsunaga ◽  
Eiichiro Fukusaki ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Nitrogen Source ◽  
Bacillus Licheniformis ◽  
Ph Value ◽  
Acid Formation

GLUTAMIC ACID FORMATION FROM GLUCOSE BY BACTERIA

1960 ◽  
Vol 47 (4) ◽  
pp. 414-421 ◽  
Author(s):  
ISAMU SHIIO ◽  
SHIN-ICHIRO OTSUKA ◽  
TOSHINAO TSUNODA
Keyword(s):  
Glutamic Acid ◽  
Acid Formation

scholarly journals Promoting Cell Growth And Poly-Y-Glutamic Acid Production By Boosting The Synthesis of Alanine And D-Alanyl-D-Alanine In Bacillus Licheniformis

2022 ◽  
Author(s):  
Zheng Zhang ◽  
Penghui He ◽  
Shiying Hu ◽  
Yanqing Yu ◽  
Xiaoting Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Microbial Biomass ◽  
Glutamic Acid ◽  
Bacillus Licheniformis ◽  
Alanine Racemase ◽  
High Yield ◽  
Effective Strategy ◽  
Genes Encoding ◽  
Alanine Synthesis ◽  
Glutamic Acid Production

Abstract Objective: The production of some bio-chemicals affected by the cell growth. This study aimed at promoting the cell growth by overexpressing the synthesis of peptidoglycans tetrapeptide tail components to improve poly-γ-glutamic acid (γ-PGA) production. Results: L-alanine, D-alanine and D-alanyl-D-alanine are primary precursors for the synthesis of peptidoglycans. The addition of L-alanine and D-alanine significantly increased both the cell growth and production of γ-PGA. Then, several genes encoding key enzymes for L/D-alanine and D-alanyl-D-alanine biosynthesis were overexpressed respectively, including ald (encoding alanine dehydrogenase), dal (encoding alanine racemase) and ddl (encoding D-alanine ligase). The results showed that the overexpression of genes ald , dal and ddl increased the production of γ-PGA by 19.72%, 15.91% and 60.90%, and increased the microbial biomass by 15.58%, 18.34% and 49.85%, respectively. Moreover, we demonstrated that the overexpression of genes ald , dal and ddl increased γ-PGA production mainly by enhancing cell growth rather than providing more precursors. Conclusions: This work illustrated the importance of the L/D-alanine and D-alanyl-D-alanine synthesis to the cell growth and the high yield of γ-PGA, and provided an effective strategy for producing γ-PGA .

Organic Acid Production by Propionibacterium shermanii: Effect of pH, Temperature and Vitamin-Nitrogen Source

1997 ◽  
Vol 52 (3-4) ◽  
pp. 193-196 ◽  
Author(s):  
A. Quesada-Chanto ◽  
A . G. Schroeder ◽  
A. C. Schmid-Meyer ◽  
J. A. López ◽  
M. M. Silveira ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Propionic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Ph Value ◽  
Corn Steep Liquor ◽  
Organic Acid Production ◽  
Propionibacterium Shermanii ◽  
Difco Yeast Extract ◽  
Steep Liquor

Abstract Production of propionic acid by Propionibacterium shermanii CDB 10014 was enhanced by a pH value of 6.5 and by temperatures in the range 35-37 °C. Depending on the type of yeast extract, succinic acid can be produced in higher proportions, with decreasing propionic acid yields. With respect to propionic acid production, Difco yeast extract has shown the best results when yeast extract preparations from other different suppliers were compared. To replace yeast extract by a cheaper vitamin-nitrogen source, corn-steep liquor was tested. A complete depletion of glucose was achieved, yielding a final propionic acid concentration of over 35 g/l. These results are even better than those obtained with Difco yeast extract and suggest the possibility of an economical process based on corn-steep liquor.

L-Glutamic Acid Formation from 2-Furoic Acid by Soil Bacteria

Nature ◽  
1964 ◽  
Vol 201 (4917) ◽  
pp. 420-421 ◽  
Author(s):  
ATSUSHI KAKINUMA ◽  
SABURŌ YAMATODANI
Keyword(s):  
Glutamic Acid ◽  
Soil Bacteria ◽  
Acid Formation ◽  
Furoic Acid
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