Cloning of various promoters for foreign gene expression in Erwinia ananas

2004 ◽  
Vol 70 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Shinichi Numata ◽  
Shinichi Ui ◽  
Masamitsu Tomiyama ◽  
Akira Hasebe ◽  
Masami Nakajima ◽  
...  
Keyword(s):  
Gene Expression ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Erwinia Ananas

scholarly journals Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors.

1990 ◽  
Vol 87 (1) ◽  
pp. 88-92 ◽  
Author(s):  
H. Daniell ◽  
J. Vivekananda ◽  
B. L. Nielsen ◽  
G. N. Ye ◽  
K. K. Tewari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Tobacco Cells ◽  
Biolistic Delivery

scholarly journals Expression Pattern of the Alpha-Kafirin Promoter Coupled with a Signal Peptide fromSorghum bicolorL. Moench

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Norazlina Ahmad ◽  
Rajnesh Sant ◽  
Milovan Bokan ◽  
Kathryn J. Steadman ◽  
Ian D. Godwin
Keyword(s):  
Gene Expression ◽  
Signal Peptide ◽  
Sweet Corn ◽  
Signal Sequence ◽  
Seed Storage ◽  
Foreign Gene ◽  
Regulatory Sequences ◽  
Fluorescent Properties ◽  
Gfp Expression ◽  
Foreign Gene Expression

Regulatory sequences with endosperm specificity are essential for foreign gene expression in the desired tissue for both grain quality improvement and molecular pharming. In this study, promoters of seed storage α-kafirin genes coupled with signal sequence (ss) were isolated fromSorghum bicolorL. Moench genomic DNA by PCR. The α-kafirin promoter (α-kaf) contains endosperm specificity-determining motifs, prolamin-box, the O2-box 1, CATC, and TATA boxes required for α-kafirin gene expression in sorghum seeds. The constructs pMB-Ubi-gfpand pMB-kaf-gfpwere microprojectile bombarded into various sorghum and sweet corn explants. GFP expression was detected on all explants using the Ubi promoter but only in seeds for the α-kaf promoter. This shows that the α-kaf promoter isolated was functional and demonstrated seed-specific GFP expression. The constructs pMB-Ubi-ss-gfpand pMB-kaf-ss-gfpwere also bombarded into the same explants. Detection of GFP expression showed that the signal peptide (SP)::GFP fusion can assemble and fold properly, preserving the fluorescent properties of GFP.

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