Insecticidal properties of Thymus persicus essential oil against Tribolium castaneum and Sitophilus oryzae

2009 ◽  
Vol 83 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Akram Taghizadeh Saroukolai ◽  
Saeid Moharramipour ◽  
Mohammad Hadi Meshkatalsadat
2008 ◽  
Vol 11 (4) ◽  
pp. 175-179 ◽  
Author(s):  
Bibi Zahra Sahaf ◽  
Saeid Moharramipour ◽  
Mohammad Hadi Meshkatalsadat

2021 ◽  
Vol 12 (2) ◽  
pp. 2262-2274

Pulicaria incisa (Lam) is a wild-growing plant in Morocco and has been traditionally used by farmers to control pests of stored grains. The present study was conducted to investigate the chemical composition and insecticidal effect of the essential oil of P. incisa against Sitophilus oryzae (L.) and Tribolium castaneum (Herbst.) by different methods (contact, fumigation and ingestion). The aerial parts of the plant were subjected to hydrodistillation using a Clevenger-type apparatus. The essential oil composition was analyzed by gas chromatography (GC) and mass spectrophotometry (MS). Sixty-six compounds representing 89.4% of total oil were identified. The main components were α-Ocimene (15.17%), τ-Cadinol (6.79%), α-Cadinol (4.51%), Alloaromadendrene (4.45%) δ-Cadinene, (+) - (4.13%). The repellant toxicity test results revealed a higher repellency effect in S. oryzae than T. castaneum. Lethal concentration (LC50), varied between 15.49 - 1.73 μL/cm2 and 20.89 - 2.29 μL/cm2 respectively. In the fumigation test, adults of S. oryzae and T. castaneum were sensitive to the essential oil with LC50 values varying between 16.21 - 2.08 and 18.62 - 2.51 μL/L air. In addition, experiments have shown that the ingestion method is the most toxic towards both insects with LC50 values of the order of 12.59 - 1.51 μL/g for S. oryzae and 14.12 - 2.39 μL/g for T. castaneum. While the lethal time (LT50) values decreased with increasing essential oil concentration, and in all cases, the increased susceptibility of both insects was directly associated with oil concentration and exposure time. This study aims to valorize medicinal and aromatic plants of the Moroccan flora in order to find novel bio-insecticidal products. Furthermore, the study reports for the first time the insecticidal activity of P. incisa against adults S. oryzae and T. castaneum.


2021 ◽  
Vol 70 (3-4) ◽  
pp. 140-148
Author(s):  
Sonja Gvozdenac ◽  
Biljana Kiprovski ◽  
Milica Aćimović ◽  
Jovana Stanković Jeremić ◽  
Mirjana Cvetković ◽  
...  

Summary The purpose of this study is to assess the repellent activity of lemongrass (Cymbopogon citratus) essential oil (EO), grown in Serbia under greenhouse conditions, against four prevalent stored product pests: Plodia interpunctella (larvae), Sitophilus oryzae, Acanthoscelides obtectus and Tribolium castaneum (adults). The lemongrass EO repellency was tested using filter paper in Petri dishes and a Y-tube olfactometer. According to the repellency index (RI), the lemongrass EO repellency was divided into 5 classes. Prior to biotesting, the chemical characterization of lemongrass EO was performed and the following main compounds were detected: myrcene (31.0%), geranial (30.0%), and neral (23.6%). The C. citratus EO considered was found to exhibit the Class III repellent activity against P. interpunctella larvae only at the highest concentration (namely 0.5%). This is the very first report on the C. citratus EO repellent activity against this pest. The lemongrass EO examined showed strong repellency (Class IV) against S. oryzae (0.2% and 0.5% of EO), A. obtectus (0.1% and 0.2%), and T. castaneum (0.05-0.1%). Moreover, higher lemongrass EO concentrations (0.5%) were found to exhibit extreme repellency (Class V) against A. obtectus and T. castaneum. The results obtained were confirmed in the bioassays performed, indicating the great potential of lemongrass EO as a bio-repellent when applied in higher concentrations to all the insects considered, regardless of the exposure period.


2018 ◽  
Vol 87 (3) ◽  
pp. 263-278 ◽  
Author(s):  
Hanane Lougraimzi ◽  
Salma El Iraqui ◽  
Abdelaaziz Bouaichi ◽  
Safae Gouit ◽  
El Hassan Achbani ◽  
...  

Abstract Stored grains are threatened by several insects, leading to losses in quality and quantity. Several studies have revealed the risks of using chemicals that can cause serious health problems for humans. It is in this perspective that the objective of our work should be seen: It was to assess the effect of the essential oil and leaf powder of Mentha pulegium against Sitophilus oryzae and Tribolium castaneum adults attacking post-harvest cereals. The insecticidal activity of the essential oil against these two insects was evaluated by three methods: contact, inhalation and ingestion. The ingestion method was used in the case of the leaf powder. The essential oil and leaf powder of Mentha pulegium exhibited insecticidal activity against Sitophilus oryzae and Tribolium castaneum adults (Mentha pulegium essential oil caused up to 100% mortality of both insects). Both insects were influenced affected by the dose, exposure time and the method by which the insecticidal activity of the essential oil was demonstrated. Adults of Sitophilus oryzae were more sensitive to different concentrations of essential oil and leaf powder than those of Tribolium castaneum. The essential oil has no effect on the germination rate of soft wheat grains (Triticum aestivum). On the contrary, it acts positively by reducing the damage caused by these major stock pests. The data from this study could present an alternative solution for replacing synthetic insecticides for the protection of stored commodities.


2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Meryem El Jemli ◽  
Naima Khattabi ◽  
Khadija Lachqer ◽  
Driss Touati ◽  
Yousra El Jemli ◽  
...  

The hydro-distilled essential oil from the leaves of Juniperus thurifera L. (JTEO) was analyzed by GC-MS and examined for its insecticidal, antifungal and antioxidant activities. A total of 51 compounds were identified. The major components were sabinene (22.4%), followed by terpinen-4-ol (11.0%) and α-pinene (5.9%). JTEO exhibited interesting insecticidal activity against adults of Acanthoscelides obtectus, Tribolium castaneum and Sitophilus oryzae, with LC50 values of 0.1, 0.6 and 2.0×10−2 μL/mL, respectively. Moreover, JTEO showed inhibitory activity against Alternaria alternata, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani and Verticillium dahlia with mycelial growth inhibition ranging from 24.0% to 92.1%. Finally, JTEO demonstrated a moderate antioxidant effect.


Author(s):  
Shanshan Gao ◽  
Haidi Sun ◽  
Jiahao Zhang ◽  
Yonglei Zhang ◽  
Peipei Sun ◽  
...  

Abstract Uridine diphosphate glucosyltransferases (UGTs), which are phase II detoxification enzymes, are found in various organisms. These enzymes play an important role in the detoxification mechanisms of plant allelopathy and in insects. Artemisia vulgaris L. (Asterales: Asteraceae: Artemisia) essential oil has strong contact toxicity to Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) larvae. However, the effect of A. vulgaris essential oil on UGTs is unclear. In this study, A. vulgaris essential oil was shown to significantly induce the expression of the TcUgt86Dg transcript. Furthermore, treatment of TcUgt86Dg-silenced individuals with A. vulgaris essential oil resulted in higher mortality than for the control individuals, indicating that TcUgt86Dg is involved in detoxification of A. vulgaris essential oil in T. castaneum. The developmental expression profile showed that the expression of TcUgt86Dg in late adults was higher than in other developmental stages. Furthermore, the expression profile in adult tissues revealed higher expression of TcUgt86Dg in the head, antenna, fat body, and accessory gland than in other tissues. These data show that TcUgt86Dg may be involved in the metabolism of exogenous toxins by T. castaneum; thus, our results have elucidated one possible mechanism of resistance to A. vulgaris essential oil and provide a theoretical basis for a control scheme for T. castaneum.


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