Effect of Olive Mill Wastewater of in the Control of Olive Verticillium Wilt Caused by Verticillium dahlia Kleb

Muhrez, K.A., M.Z. Tawil and B. Barhoum. 2021. Effect of Olive Mill Wastewater of in the Control of Olive Verticillium Wilt Caused by Verticillium dahlia Kleb. Arab Journal of Plant Protection, 39(4): 273-280. https://doi.org/10.22268/AJPP-39.4.273280 This research aimed to evaluate the application of olive mill wastewater in controlling Verticillium dahliae the causal agent of olive wilt disease on olive trees. This study was carried out on 2 years old olive local variety (Khderi) plants planted in pots after it has been artificial inoculated with V. dahliae, and severity was recorded within 90 days. Olive mill wastewater (omww) efficacy was estimated at the end of the experiment. Severity was increased from 8.89% before treatment to 28.89% 60 days after treatment, and decreased to 13.33% 90 days after treatment with omww 450 ml/l. Disease severity increased from 11.11% before treatment to 37.78% 60 days after treatment and decreased to 35.56% 90 days after treatment with omww 300 ml/l. Whereas, disease severity increased from 17.78% before treatment to 40% 90 days after treatment with azoxystrobin compared to the inoculated control where disease severity increased from 13.33% before treatment to 100% 90 days after treatment. Omww 450 ml/l had the highest efficacy of 80% followed by azoxystrobin 70%, and omww 300 ml/l 57.33%. It can be concluded that olive mill wastewater has a potential application as a bio-fungicide for the control of olive verticillium wilt disease. Keywords: Olive mill waste waters, Verticillium dahlia, olive, Fungicide.

Mycobiota of the upper layer of pine forests litter in the parks of Surgut in the environmental assessment of anthropogenic impact on these territories

The paper studies mycobiota of the upper layer of pine forests litter in the parks of Surgut: Za Saimoy, Energetikov, Kedrovy Log, Neftyanik. The author identified 52 isolates of soil microscopic fungi belonging to 14 genera. Most of the isolates - anamorphic (mitospore) fungi - are represented by 9 genera: Acremonium , Annellosporium , Aspergillus , Fusarium , Humicola , Penicillium , Trichoderma , Ulocladium , Verticillium . Zygomycetes belong to three genera - Absidia , Mucor and Umbelopsis . Ascomycetes are represented by two genera: Eupenicillium and Talaromyces . The author also isolated Mycelia sterilia group. Representatives of the genera Absidia , Trichoderma , Fusarium and Penicillium are found in the mycocenoses of the studied parks. The genera Trichoderma , Fusarium and Penicillium also dominate by the number of isolates. Umbelopsis isabellina species is abundant in the Kedrovy Log park. Species of the genus Trichoderma are abundant in the Neftyanik park. Light-colored micromycetes of the group Mycelia sterilia are found and abundant only in the Za Saimoy park. Cellulolytics of the genus Trichoderma and the species Annellosporium nemorosum are abundant in the Energetikov park. Thus, mycobiota of the upper layer litter of the studied parks is close to the mycobiota of the background podzolic soils. It contains fungal species typical of the background soils. Fungi resistant to anthropogenic influences - dark-colored micromycetes Ulocladium alternarie and phytopathogenic species Verticillium dahlia var. longisporum . were found in the minority only in the Energetikov park. The obtained data allow us to conclude that the anthropogenic influence on the structure of the mycocenosis of the Energetikov park is more pronounced, but in general it does not have a significant effect on the mycobiota of the upper layer litter of the studied parks.

Novel Thiochromanone Derivatives Containing a Sulfonyl Hydrazone Moiety: Design, Synthesis, and Bioactivity Evaluation

A series of novel thiochromanone derivatives containing a sulfonyl hydrazone moiety were designed and synthesized. Their structures were determined by 1H-NMR, 13C-NMR, and HRMS. Bioassay results showed that most of the target compounds revealed moderate to good antibacterial activities against Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicolaby, and Xanthomonas axonopodis pv. citri. Compound 4i had the best inhibitory activity against Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicolaby, and Xanthomonas axonopodis pv. citri, with the EC50 values of 8.67, 12.65, and 10.62 μg/mL, which were superior to those of Bismerthiazol and Thiodiazole-copper. Meanwhile, bioassay results showed that all of the target compounds proved to have lower antifungal activities against Sclerotinia sclerotiorum, Fusarium oxysporum, Gibberella zeae, Rhizoctonia solani, Verticillium dahlia, and Botrytis cinerea than those of Carbendazim.

Coniferous Diseases In The Andijan Region

The article provides data on the composition of diseases of decorative coniferous crops in the Andizhan region. In total, 11 species of micromycetes were identified. In the nursery, during the lodging of seedlings, species of the river were noted. Fusarium: Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc., Verticillium dahlia Klebn. By the frequency of occurrence, Fusarium blight was noted much more often. Lodging of seedlings was also caused by Pythium debaryanum Hesse. Among the diseases of adult plants, we have identified - rust juniper (exc. - Gymnosporangium confusum Plowr.), defeat of needles (phomosis, rust), shoots and branches (diplodiosis, phomosis). drying of needles and juniper shoots and black needles (Hormiscium pinophilum (Nees.) Lind., Fumago vagans Pers.).

Some Plants Showing Antagonism to Five Plant Pathogenic Fungi

Fungi are among the main agents of plant diseases, being responsible for major losses in agriculture. The control of these microorganisms carried out using chemical compounds and numerous cases of resistance have already been observed, which makes it necessary to search for alternative methods of management of these pathogens. Therefore, the aim of this study, to evaluate the antifungal potential in plants. Twenty-four (24) plant extracts were tested for their antifungal potential against five plant pathogenic fungi: Sclerotinia sclerotiorum (Ss), Stromatinia cepivora (Sc), Fusarium oxysporum (Fox), Colletotrichum gloesporioides (Cg) and Verticillium dahlia (Vd). For the evaluation of the fungicidal potential, plant extracts were prepared by liquefying the plants in distilled water. The extracts were incorporated into PDA (Potato-Dextrose-Agar) culture medium to a final concentration of 35% and autoclaved. Then, PDA discs colonized by the aforementioned fungi were added to the center of each plate with the respective treatments (plant extracts). When all control treatments (PDA medium without plant extract) had colonized the entire Petri dish, the diameters of the fungal colonies were measured to calculate the Mycelial Growth Inhibition Index (MGI). Tests showed that all these plant extracts have some antifungal activity, ranging from 0 to 100% inhibition. In general, extracts of basil, lavender, guaco, rue, toxic cassava and black plum were the ones that stood out, with MGIs above 50%. New studies are being conducted to evaluate the activity of plant extracts without autoclaving, inhibition of sclerotia formation, to determine the minimum inhibitory concentration, as well as other parts of plants like roots and seeds, mixtures of plant extracts and in vivo antagonism tests.

The Cotton miR477-CBP60A Module Participates in Plant Defense Against Verticillium dahlia

Previous reports have shown that, when Verticillium dahliae localizes at the root surface, many microRNAs (miRNAs) were identified at the early induction stage. Here, we constructed two groups from two timepoints of small RNA (sRNA) in cotton root responses to V. dahliae at the later induction stage, pathogen localizing in the interior of root tissue. We identified 71 known and 378 novel miRNAs from six libraries of the pathogen-induced and the control sRNAs. Combined with degradome and sRNA sequencing, 178 corresponding miRNA target genes were identified, in which 40 target genes from differentially expressed miRNAs were primarily associated with oxidation-reduction and stress responses. More importantly, we characterized the cotton miR477-CBP60A module in the later response of the plant to V. dahliae infection. A β-glucuronidase fusion reporter and cleavage site analysis showed that ghr-miR477 directly cleaved the messenger RNA of GhCBP60A in the posttranscriptional process. The ghr-miR477-silencing decreased plant resistance to this fungus, while the knockdown of GhCBP60A increased plant resistance, which regulated GhICS1 expression to determine salicylic acid level. Our data documented that numerous later-inducible miRNAs in the plant response to V. dahliae, suggesting that these miRNAs play important roles in plant resistance to vascular disease.

Complete Genome Sequence of Bacillus velezensis Strain AL7, a Biocontrol Agent for Suppression of Cotton Verticillium Wilt

Bacillus velezensis AL7, isolated from cotton soil, had strong antagonistic activity to Verticillium dahlia Kleb. The AL7 genome consisted of one chromosome with 3,894,709 bp (46.64% G+C content). Genome annotation predicted 3,706 protein-coding genes, 86 tRNAs, and 27 rRNAs. We sequenced and annotated the complete AL7 genome to help us better understand use of this strain.

Coelomic Fluid of Earthworm, Eudrilus Eugeniae, Inhibits the Growth of Fungal Hyphae, in Vitro

Knowledge on interactions between earthworms and microbes at molecular level has been obscure. Mechanism by which earthworms defend themselves against different microorganisms in the soil is largely unknown. Coelomic fluid from earthworm species has been shown to have antimicrobial activity. In this study we report that coelomic fluid isolated from Eudrilus eugeniae has antifungal activity against four different phytopathogens such as Rhizoctonia solani, Fusarium oxysporum, Aspergillus flavus and Verticillium dahlia. Incubation of coelomic fluid with fungal hyphae of all these four strains for 12 hours revealed that the growth of hyphae was greatly inhibited. Light microscopy studies confirmed that the hyphae growth was greatly reduced by incubation of coelomic fluid. Analysis of the fluid in the microscope showed the presence of different types of coelomocytes in the mucous fluid. Other molecules and compounds in the coelomic fluid might also have played role in antifungal activity.