A Quantitative Real-Time PCR Approach for the Detection and Characterization of Endothelial Cells in Whole Blood

2011 ◽  
Vol 39 (10) ◽  
pp. 2627-2636 ◽  
Author(s):  
Sudhir P. Deosarkar ◽  
Pooja Bhatt ◽  
Christopher J. Lewis ◽  
Douglas J. Goetz
Keyword(s):  
Endothelial Cells ◽  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Quantitative Real Time Pcr

scholarly journals Improved HF183 Quantitative Real-Time PCR Assay for Characterization of Human Fecal Pollution in Ambient Surface Water Samples

2014 ◽  
Vol 80 (10) ◽  
pp. 3086-3094 ◽  
Author(s):  
Hyatt C. Green ◽  
Richard A. Haugland ◽  
Manju Varma ◽  
Hana T. Millen ◽  
Mark A. Borchardt ◽  
...  
Keyword(s):  
Real Time ◽  
Surface Waters ◽  
Real Time Pcr ◽  
Fecal Pollution ◽  
Qpcr Assay ◽  
Taqman Assay ◽  
Reference Database ◽  
Quantitative Real Time Pcr ◽  
Ambient Surface

ABSTRACTQuantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genusBacteroidesare among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a modified TaqMan assay (HF183/BacR287) that alleviates this problem. The performance of each qPCR assay was compared in head-to-head experiments investigating limits of detection, analytical precision, predicted hybridization to 16S rRNA gene sequences from a reference database, and relative marker concentrations in fecal and sewage samples. The performance of the modified HF183/BacR287 assay is equal to or improves upon that of the original HF183/BFDrev assay. In addition, a qPCR chemistry designed to combat amplification inhibition and a multiplexed internal amplification control are included. In light of the expanding use of PCR-based methods that rely on the detection of extremely low concentrations of DNA template, such as qPCR and digital PCR, the new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.

Characterization of fecal concentrations in human and other animal sources by physical, culture-based, and quantitative real-time PCR methods

2013 ◽  
Vol 47 (18) ◽  
pp. 6873-6882 ◽  
Author(s):  
Jared S. Ervin ◽  
Todd L. Russell ◽  
Blythe A. Layton ◽  
Kevan M. Yamahara ◽  
Dan Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Physical Culture ◽  
Quantitative Real Time Pcr

Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Anoectochilus roxburghii

2011 ◽  
Vol 39 (5) ◽  
pp. 5905-5912 ◽  
Author(s):  
Gang Zhang ◽  
Mingming Zhao ◽  
Chao Song ◽  
Anxiong Luo ◽  
Jianfa Bai ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr ◽  
Anoectochilus Roxburghii

Design and development of a quantitative real time PCR assay for monitoring of HTLV-1 provirus in whole blood

2012 ◽  
Vol 53 (4) ◽  
pp. 302-307 ◽  
Author(s):  
Mahmood Naderi ◽  
Mahdi Paryan ◽  
Kayhan Azadmanesh ◽  
Houshang Rafatpanah ◽  
Houri Rezvan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Design And Development

Molecular Characterization of Large Deletions in the von Hippel-Lindau (VHL) Gene by Quantitative Real-Time PCR

2006 ◽  
Vol 10 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Alberto Casarin ◽  
Maddalena Martella ◽  
Roberta Polli ◽  
Emanuela Leonardi ◽  
Laura Anesi ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Vhl Gene ◽  
Von Hippel Lindau ◽  
Large Deletions
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