Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree

2015 ◽  
Vol 89 (4) ◽  
pp. 721-733 ◽  
Author(s):  
S. Perveen ◽  
M. Anis
Keyword(s):  
Biochemical Parameters ◽  
Albizia Lebbeck ◽  
Ex Vitro ◽  
Physiological And Biochemical

scholarly journals Evaluation of Indigenous Olive Biocontrol Rhizobacteria as Protectants against Drought and Salt Stress

2021 ◽  
Vol 9 (6) ◽  
pp. 1209
Author(s):  
Nuria Montes-Osuna ◽  
Carmen Gómez-Lama Cabanás ◽  
Antonio Valverde-Corredor ◽  
Garikoitz Legarda ◽  
Pilar Prieto ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stresses ◽  
Biochemical Parameters ◽  
Biocontrol Agent ◽  
Negative Effects ◽  
Experimental Conditions ◽  
Acds Gene ◽  
Drought And Salt Stress ◽  
Physiological And Biochemical

Stress caused by drought and salinity may compromise growth and productivity of olive (Olea europaea L.) tree crops. Several studies have reported the use of beneficial rhizobacteria to alleviate symptoms produced by these stresses, which is attributed in some cases to the activity of 1-aminocyclopropane-1-carboxylic acid deaminase (ACD). A collection of beneficial olive rhizobacteria was in vitro screened for ACD activity. Pseudomonas sp. PICF6 displayed this phenotype and sequencing of its genome confirmed the presence of an acdS gene. In contrast, the well-known root endophyte and biocontrol agent Pseudomonas simiae PICF7 was defective in ACD activity, even though the presence of an ACD-coding gene was earlier predicted in its genome. In this study, an unidentified deaminase was confirmed instead. Greenhouse experiments with olive ‘Picual’ plants inoculated either with PICF6 or PICF7, or co-inoculated with both strains, and subjected to drought or salt stress were carried out. Several physiological and biochemical parameters increased in stressed plants (i.e., stomatal conductance and flavonoids content), regardless of whether or not they were previously bacterized. Results showed that neither PICF6 (ACD positive) nor PICF7 (ACD negative) lessened the negative effects caused by the abiotic stresses tested, at least under our experimental conditions.

Physiological and Biochemical Responses of Merwilla plumbea Cultured In Vitro with Different Cytokinins After 1 Year of Growth Under Ex Vitro Conditions

2016 ◽  
Vol 36 (1) ◽  
pp. 83-95
Author(s):  
Mack Moyo ◽  
Stephen O. Amoo ◽  
Adeyemi O. Aremu ◽  
Lucie Plíhalová ◽  
Jiří Gruz ◽  
...  
Keyword(s):  
Ex Vitro ◽  
Biochemical Responses ◽  
Physiological And Biochemical

scholarly journals Physiological and biochemical changes of micropropagated Dendrobium anosmum Lindl. in ex vitro acclimatization process

2019 ◽  
Vol 2 (3) ◽  
pp. 59-67
Author(s):  
Bang Phi Cao
Keyword(s):  
Water Content ◽  
Environmental Changes ◽  
Photochemical Efficiency ◽  
Biochemical Changes ◽  
Ex Vitro ◽  
In Vitro Plantlets ◽  
Relative Water ◽  
Leaf Relative Water Content ◽  
Physiological And Biochemical

The ex vitro acclimatization process plays an important role in plant micropropagation. In vitro plantlets have to rapidly adapt to environmental changes. The current work aimed at assessing some physiological and biochemical changes of micropropagated Dendrobium anosmum Lindl. Plantlets during ex vitro acclimatization process, eg. contents of water (leaf relative water content), dry matter, proline and photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoid), chlorophyll fluorescence and antioxidant enzymes (peroxidase và catalase) activities. The analyzed results showed that water content decreased in acclimatized plantlets compared to in vitro ones. The chlorophylls and carotenoids contents of what were significantly higher in ex vitro plantlet leaves compared to the day 0 plantlets. The pigment contents were observed to increase during the ex vitro acclimatation process. When the plantlets were moved out of the in vitro medium, the maximum photochemical efficiency of photosystem II (Fv/Fm) significantly decreased at the early acclimatation points then restored at the end of acclimatation process. The content of proline and activities of antoxidant enzymes significantly increased with different periods of acclimatation process. The proline content and enzyme activities were recorded at the first ex vitro period when most water loss occurred in plantlets. These results suggest that Dendrobium anosmum Lindl in vitro plantlets have adapted to the transplantation by possesing some physiological responses of its photosynthetic system as well as its antioxidant machinery.

scholarly journals EFFECT OF CALCIUM THE PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF LARGE-BED HYDRANGEA (HYDRANGEA MACROPHYLLA SER.) IN A SLOW-GROWING CULTURE IN VITRO

2020 ◽  
Vol 6 (66) ◽  
pp. 270-283
Author(s):  
Valentina Ivanovna Malyarovskaya ◽  
◽  
Lyudmila Stepanovna Malyukova ◽  
Natalia Grigorievna Koninskaya ◽  
◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Culture In Vitro ◽  
Hydrangea Macrophylla ◽  
Slow Growing ◽  
Physiological And Biochemical

scholarly journals Evaluation of some physiological and biochemical parameters of Camelina sativa seedlings under osmotic stress

2019 ◽  
Vol 25 ◽  
pp. 287-292
Author(s):  
L. V. Nishchenko ◽  
L. O. Sakhno
Keyword(s):  
Superoxide Dismutase ◽  
Osmotic Stress ◽  
Genetic Transformation ◽  
Biochemical Parameters ◽  
Camelina Sativa ◽  
Total Soluble Protein ◽  
Fresh Weight ◽  
Sod Activity ◽  
Physiological And Biochemical

Aim. To find the most drought resistant spring camelina (Camelina sativa (L.) Crantz) genotype for further involvement in genetic transformation experiments, the some physiological and biochemical parameters of seedlings have been investigated under normal conditions and osmotic stress in vitro. Methods. Sterile seeds were planted on Murashige and Skoog agarized medium without hormones or the same media supplemented with mannitol as an osmotic stress inductor. Germination, fresh weight, total soluble protein content, and superoxide dismutase (SOD) activity have been investigated in 7-day-old seedlings. Results. The analyzed camelina genotypes were differed in their reactions on water deficit in vitro. It has been revealed that higher SOD activity of FEORZhYaF-1 seedlings was accompanied by higher germination and higher ability to fresh weight accumulation under osmotic stress as compared to other varieties. Conclusions. FEORZhYaF-1 breeding form could be involved in genetic transformation experiments to improve drought resistance. SOD activity should be used as a parameter for selection of osmotic stress resistant plant material. Keywords: Camelina sativa (L.) Crantz  drought resistance, superoxide dismutase, total soluble protein.

Albactam, a novel β- lactam derivative from the flowers of Albizia lebbeck with platelets anti-aggregatory activity in vitro

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
A El Gamal ◽  
M Abd El Halim ◽  
A Khalil ◽  
O Basoudan ◽  
A Al-Rehaily ◽  
...  
Keyword(s):  
Albizia Lebbeck

Effects of Allochthonous Nitrogen on Some Physiological and Biochemical Parameters in Carps

2007 ◽  
Vol 43 (1) ◽  
pp. 95-105
Author(s):  
O. G. Zinkovskiy ◽  
V. D. Solomatina ◽  
A. S. Potrokhov ◽  
N. A. Mogilevich
Keyword(s):  
Biochemical Parameters ◽  
Physiological And Biochemical

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

2017 ◽  
Vol 35 (1-2) ◽  
pp. 73-82
Author(s):  
Carlos Iván Espinosa ◽  
Gabriel Ríos
Keyword(s):  
Ex Vitro

El uso de herramientas biotecnológicas como la micropropagación se constituye en una alternativa de reproducción de especies amenazadas y con tamaños poblacionales reducidos. Sin embargo, uno de los problemas críticos en el uso de la micropropagación como herramienta de reproducción es la calidad de las plántulas resultantes en cuanto a su crecimiento y vigor. En el presente trabajo se evalua los efectos de la micropropagación sobre los patrones de crecimiento y sobrevivencia de plán­tulas in vitro de Cinchona officinalis L., una especie que ha sido fuertemente impactada por procesos de tala dentro de bosques naturales durante la época de la colonia. Se realizó un monitoreo de un total de 120 plántulas in vitro y 1988 plántulas ex vitro por 8 meses a partir del último repique. Adi­cionalmente, en cada plántula se contabilizó la cantidad de brotes axilares. Los resultados obtenidos mostraron un efecto remanente de los procesos de micropropagación, los cuales inicialmente inciden en la cantidad de brotes de las plántulas y en el crecimiento; sin embargo, este efecto no influye de forma negativa en la sobrevivencia de las plántulas durante la fase ex vitro

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