Physiological and Biochemical Responses of Merwilla plumbea Cultured In Vitro with Different Cytokinins After 1 Year of Growth Under Ex Vitro Conditions

2016 ◽  
Vol 36 (1) ◽  
pp. 83-95
Author(s):  
Mack Moyo ◽  
Stephen O. Amoo ◽  
Adeyemi O. Aremu ◽  
Lucie Plíhalová ◽  
Jiří Gruz ◽  
...  
Keyword(s):  
Ex Vitro ◽  
Biochemical Responses ◽  
Physiological And Biochemical

Physiological and biochemical responses of in vitro Fusarium oxysporum f.sp. niveum to benzoic acid

2009 ◽  
Vol 54 (2) ◽  
pp. 115-122 ◽  
Author(s):  
H. -S. Wu ◽  
Y. Wang ◽  
C. -Y. Zhang ◽  
M. Gu ◽  
Y. -X. Liu ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Biochemical Responses ◽  
Physiological And Biochemical

scholarly journals Physiological and biochemical changes of micropropagated Dendrobium anosmum Lindl. in ex vitro acclimatization process

2019 ◽  
Vol 2 (3) ◽  
pp. 59-67
Author(s):  
Bang Phi Cao
Keyword(s):  
Water Content ◽  
Environmental Changes ◽  
Photochemical Efficiency ◽  
Biochemical Changes ◽  
Ex Vitro ◽  
In Vitro Plantlets ◽  
Relative Water ◽  
Leaf Relative Water Content ◽  
Physiological And Biochemical

The ex vitro acclimatization process plays an important role in plant micropropagation. In vitro plantlets have to rapidly adapt to environmental changes. The current work aimed at assessing some physiological and biochemical changes of micropropagated Dendrobium anosmum Lindl. Plantlets during ex vitro acclimatization process, eg. contents of water (leaf relative water content), dry matter, proline and photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoid), chlorophyll fluorescence and antioxidant enzymes (peroxidase và catalase) activities. The analyzed results showed that water content decreased in acclimatized plantlets compared to in vitro ones. The chlorophylls and carotenoids contents of what were significantly higher in ex vitro plantlet leaves compared to the day 0 plantlets. The pigment contents were observed to increase during the ex vitro acclimatation process. When the plantlets were moved out of the in vitro medium, the maximum photochemical efficiency of photosystem II (Fv/Fm) significantly decreased at the early acclimatation points then restored at the end of acclimatation process. The content of proline and activities of antoxidant enzymes significantly increased with different periods of acclimatation process. The proline content and enzyme activities were recorded at the first ex vitro period when most water loss occurred in plantlets. These results suggest that Dendrobium anosmum Lindl in vitro plantlets have adapted to the transplantation by possesing some physiological responses of its photosynthetic system as well as its antioxidant machinery.

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

2017 ◽  
Vol 35 (1-2) ◽  
pp. 73-82
Author(s):  
Carlos Iván Espinosa ◽  
Gabriel Ríos
Keyword(s):  
Ex Vitro

El uso de herramientas biotecnológicas como la micropropagación se constituye en una alternativa de reproducción de especies amenazadas y con tamaños poblacionales reducidos. Sin embargo, uno de los problemas críticos en el uso de la micropropagación como herramienta de reproducción es la calidad de las plántulas resultantes en cuanto a su crecimiento y vigor. En el presente trabajo se evalua los efectos de la micropropagación sobre los patrones de crecimiento y sobrevivencia de plán­tulas in vitro de Cinchona officinalis L., una especie que ha sido fuertemente impactada por procesos de tala dentro de bosques naturales durante la época de la colonia. Se realizó un monitoreo de un total de 120 plántulas in vitro y 1988 plántulas ex vitro por 8 meses a partir del último repique. Adi­cionalmente, en cada plántula se contabilizó la cantidad de brotes axilares. Los resultados obtenidos mostraron un efecto remanente de los procesos de micropropagación, los cuales inicialmente inciden en la cantidad de brotes de las plántulas y en el crecimiento; sin embargo, este efecto no influye de forma negativa en la sobrevivencia de las plántulas durante la fase ex vitro

Physiological and biochemical responses of harvested rambutan (Nephelium lappaceum L.) fruits to postharvest dip treatments during storage

2018 ◽  
Vol 19 (1&2) ◽  
pp. 217-222
Author(s):  
Manjunath J. Shetty ◽  
◽  
P.R. Geethalekshmi ◽  
C. Mini ◽  
Vijayaraghava Kumar ◽  
...  
Keyword(s):  
Biochemical Responses ◽  
Nephelium Lappaceum ◽  
Physiological And Biochemical

Long-term in vitro Storage Technique of Valuable Birch Genotypes and Plant Production on its Basis

2019 ◽  
pp. 57-67
Author(s):  
T.M. Tabatskaya ◽  
N.I. Vnukova
Keyword(s):  
Plant Production ◽  
High Survival ◽  
Ex Vitro ◽  
Regenerative Capacity ◽  
In Vitro Storage ◽  
Interspecific Differences ◽  
Regenerated Plants ◽  
Growing Conditions

A technique for the long-term (up to 27 years) in vitro storage of valuable birch genotypes under normal (25 °C, 2.0 klx, 16-h day and 8-h night) and low temperature (4 °C, 0.5 klx, 6-h day and 18-h night) growing conditions on hormone-free media has been described. The study explored for the first time the influence of different strategies to store the clones of Betula pubescens and B. pendula var. сarelica (6 genotypes) on the regenerative capacity of collection samples, adaptive potential of regenerated plants and plant production by the in vitro and ex vitro techniques. It was established that both storage strategies provided a persistently high survival rate (82-100%) and regenerative capacity of in vitro shoots (the multiplication coefficient of 4.2-6.3 and rhizogenic activity of 90-100%). The clones retained their characteristics of height growth under the in vitro and ex vitro conditions, and demonstrated intraclonal homogeneity and lack of signs of somaclonal variability. The plants showed substantial interspecific differences at the stage of multiplication and transfer to the greenhouse. The highest percentage of acclimated plants (75-98% depending on the clone genotype) was obtained after planting of micro plants straight in the greenhouse, which simplified the technology and made plant production less costly. long-term in vitro storage, birch, species, genotype, micropropagation, ex vitro adaptation, plant material

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