Interactions between mineral fertilization and arbuscular mycorrhizal fungi improve nursery growth and drought tolerance of Prosopis alba seedlings

Mitogen-activated protein kinase (MAPK) cascades play important roles in the stress response in both plants and microorganisms. The mycorrhizal symbiosis established between arbuscular mycorrhizal fungi (AMF) and plants can enhance plant drought tolerance, which might be closely related to the fungal MAPK response and the molecular dialogue between fungal and soybean MAPK cascades. To verify the above hypothesis, germinal Glomus intraradices (syn. Rhizophagus irregularis) spores and potted experiments were conducted. The results showed that AMF GiMAPKs with high homology with MAPKs from Saccharomyces cerevisiae had different gene expression patterns under different conditions (nitrogen starvation, abscisic acid treatment, and drought). Drought stress upregulated the levels of fungi and soybean MAPK transcripts in mycorrhizal soybean roots, indicating the possibility of a molecular dialogue between the two symbiotic sides of symbiosis and suggesting that they might cooperate to regulate the mycorrhizal soybean drought-stress response. Meanwhile, the changes in hydrogen peroxide, soluble sugar, and proline levels in mycorrhizal soybean as well as in the accelerated exchange of carbon and nitrogen in the symbionts were contributable to drought adaptation of the host plants. Thus, it can be preliminarily inferred that the interactions of MAPK signals on both sides, symbiotic fungus and plant, might regulate the response of symbiosis and, thus, improve the resistance of mycorrhizal soybean to drought stress.

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Nitrogen capture by grapevine roots and arbuscular mycorrhizal fungi from legume cover-crop residues under low rates of mineral fertilization

Biology and Fertility of Soils ◽

10.1007/s00374-008-0281-7 ◽

2008 ◽

Vol 44 (7) ◽

pp. 965-973 ◽

Cited By ~ 5

Author(s):

Xiaomei Cheng ◽

Amy Euliss ◽

Kendra Baumgartner

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Cover Crop ◽

Crop Residues ◽

Arbuscular Mycorrhizal ◽

Mineral Fertilization ◽

Legume Cover Crop

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Combined Heat and Drought Tolerance of Tomato Plants when Treated with different Arbuscular Mycorrhizal Fungi

Proceedings of the 6th World Congress on Civil, Structural, and Environmental Engineering ◽

10.11159/iceptp21.lx.101 ◽

2021 ◽

Author(s):

Imane Haddidi ◽

Bence Gajdics ◽

Nguyen Hong Duc ◽

Katalin Posta

Keyword(s):

Drought Tolerance ◽

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Tomato Plants

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Differential responses of arbuscular mycorrhizal fungal communities to long-term fertilization in the wheat rhizosphere and root endosphere

Applied and Environmental Microbiology ◽

10.1128/aem.00349-21 ◽

2021 ◽

Author(s):

Yuying Ma ◽

Huanchao Zhang ◽

Daozhong Wang ◽

Xisheng Guo ◽

Teng Yang ◽

...

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Rhizosphere Soil ◽

Organic Amendments ◽

Alpha Diversity ◽

Arbuscular Mycorrhizal ◽

Mineral Fertilization ◽

Amf Communities ◽

Arbuscular Mycorrhizal Fungal

Arbuscular mycorrhizal fungi (AMF) provide essential nutrients to crops and are critically impacted by fertilization in agricultural ecosystems. Understanding shifts in AMF communities in and around crop roots under different fertilization regimes can provide important lessons for improving agricultural production and sustainability. Here, we compared the responses of AMF communities in the rhizosphere (RS) and root endosphere (ES) of wheat ( Triticum aestivum ) to different fertilization treatments: Non-fertilization (Control), mineral fertilization only (NPK), mineral fertilization plus wheat straw (NPKS), and mineral fertilization plus cow manure (NPKM). We employed high-throughput amplicon sequencing and investigated the diversity, community composition, and network structure of AMF communities to assess their responses to fertilization. Our results elucidated that AMF communities in the RS and ES respond differently to fertilization schemes. Long-term NPK application decreased the RS AMF alpha diversity significantly, whereas additional organic amendments (straw or manure) had no effect. Contrastingly, NPK fertilization increased the ES AMF alpha diversity significantly, while additional organic amendments decreased it significantly. The effect of different fertilization schemes on AMF network complexity in the RS and ES were similar to their effects on alpha diversity. Changes to AMF communities in the RS and ES correlated mainly with the pH and phosphorus level of the rhizosphere soil under long-term inorganic and organic fertilization regimes. We suggest that the AMF community in the roots should be given more consideration when studying the effects of fertilization regimes on AMF in agroecosystems. Importance Arbuscular mycorrhizal fungi are an integral component of rhizospheres, bridging the soil and plant systems and are highly sensitive to fertilization. However, surprisingly little is known about how the response differs between the roots and the surrounding soil. Decreasing arbuscular mycorrhizal fungal diversity under fertilization has been reported, implying a potential reduction in the mutualism between plants and arbuscular mycorrhizal fungi. However, we found opposing responses to long-term fertilization managements of arbuscular mycorrhizal fungi in the wheat roots and rhizosphere soil. These results suggested that changes in the arbuscular mycorrhizal fungal community in soils do not reflect those in the roots, highlighting that the root arbuscular mycorrhizal fungal community is pertinent to understand arbuscular mycorrhizal fungi and their crop hosts’ responses to anthropogenic influences.

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Arbuscular Mycorrhizal Fungi Mediate Drought Tolerance and Recovery in Two Contrasting Carob (Ceratonia siliqua L.) Ecotypes by Regulating Stomatal, Water Relations, and (In)Organic Adjustments

Plants ◽

10.3390/plants9010080 ◽

2020 ◽

Vol 9 (1) ◽

pp. 80 ◽

Cited By ~ 11

Author(s):

Abderrahim Boutasknit ◽

Marouane Baslam ◽

Mohamed Ait-El-Mokhtar ◽

Mohamed Anli ◽

Raja Ben-Laouane ◽

...

Keyword(s):

Stomatal Conductance ◽

Drought Tolerance ◽

Arbuscular Mycorrhizal Fungi ◽

Oxidative Damage ◽

Water Content ◽

Mycorrhizal Fungi ◽

Early Stage ◽

Arbuscular Mycorrhizal ◽

Ceratonia Siliqua ◽

Improvement Programs

Irregular precipitation and drought caused an increase in tree mortality rates in multiple forest biomes with alterations in both ecosystem services and carbon balance. Carob (Ceratonia siliqua) growth and production in arid and semi-arid ecosystems are likely affected by climate change-induced droughts. Understanding the physiological responses of drought-induced early-stage tree death and strategies to enhance drought tolerance and optimize growth will help tree improvement programs. Mycorrhizal inoculation has a pronounced impact on plant growth, water absorption, mineral nutrition, and protection from abiotic stresses. However, a better understanding of these complex interconnected cellular processes and arbuscular mycorrhizal fungi (AMF)-mediated mechanisms regulating drought tolerance in plants will enhance its potential application as an efficient approach for bio-amelioration of stresses. The objectives of this work were to elucidate the different effects of autochthone AMF on inorganic solute and water content uptakes, organic adjustments (sugar and proteins content), leaf gas exchange (stomatal conductance and efficiency of photosystems I and II), and oxidative damage of two contrasting ecotypes of carob seedlings: coastal (southern ecotype (SE)) and in-land (northern ecotype (NE)) under control (C), drought (by cessation of irrigation for 15 days (15D)), and recovery (R) conditions. Our findings showed that AMF promoted growth, nutrient content, and physiological and biochemical parameters in plants of both ecotypes during C, 15D, and R conditions. After four days of recovery, stomatal conductance (gs), the maximum photochemical efficiency of PSII (Fv/Fm), water content, and plant uptake of mineral nutrients (P, K, Na, and Ca) were significantly higher in shoots of mycorrhizal (AM) than non-mycorrhizal (NM) control plants. Consequently, AMF reduced to a greater degree the accumulation of hydrogen peroxide (H2O2) and oxidative damage to lipid (malondialdehyde (MDA)) content in AM than NM plants in NE and SE, after recovery. Altogether, our findings suggest that AMF can play a role in drought resistance of carob trees at an early stage by increasing the inorganic solutes (P, K, Na, and Ca), water content uptake, organic solutes (soluble sugars and protein content), stomatal conductance, and defense response against oxidative damage during re-watering after drought stress.

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