Aquatic birnavirus capsid protein, VP3, induces apoptosis via the Bad-mediated mitochondria pathway in fish and mouse cells

Mouse cells which normally permitted polyoma virus development, and hamster cells which were non-permissive (BHK-21 and polyoma virus-transformed hamster cells), were subjected to cell-fusion techniques, and the resultant cultures were examined for the capacity of heterokaryons to yield polyoma viral capsid protein. Little or no capsid protein was synthesized in the heterokaryons, although individual mouse cells and mouse homokaryons gave normal yields. Furthermore, the hamster cell inhibitory effect manifested itself even when the mouse cells had been infected with the virus before cell fusion.

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U.V.-enhanced Reactivation of Capsid Protein Synthesis and Infectious Centre Formation in Mouse Cells Infected with U.V.-irradiated Minute-Virus-of-Mice

International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine ◽

10.1080/09553008214550131 ◽

1982 ◽

Vol 41 (2) ◽

pp. 119-126 ◽

Cited By ~ 3

Author(s):

Luo Zu Yu ◽

J.J. Cornelis ◽

J.M. Vos ◽

J. Rommelaere

Keyword(s):

Protein Synthesis ◽

Capsid Protein ◽

Minute Virus Of Mice ◽

Minute Virus ◽

Mouse Cells

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Some Structural Features of Metaphase Chromosomes of Mice and Men

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060969 ◽

1967 ◽

Vol 25 ◽

pp. 190-191

Author(s):

Godfrey C. Hoskins ◽

Betty B. Hoskins

Keyword(s):

Electron Microscopy ◽

Electron Micrographs ◽

Structural Features ◽

Metaphase Chromosomes ◽

The Future ◽

Of Mice And Men ◽

Mouse Cells ◽

Human And Mouse

Metaphase chromosomes from human and mouse cells in vitro are isolated by micrurgy, fixed, and placed on grids for electron microscopy. Interpretations of electron micrographs by current methods indicate the following structural features.Chromosomal spindle fibrils about 200Å thick form fascicles about 600Å thick, wrapped by dense spiraling fibrils (DSF) less than 100Å thick as they near the kinomere. Such a fascicle joins the future daughter kinomere of each metaphase chromatid with those of adjacent non-homologous chromatids to either side. Thus, four fascicles (SF, 1-4) attach to each metaphase kinomere (K). It is thought that fascicles extend from the kinomere poleward, fray out to let chromosomal fibrils act as traction fibrils against polar fibrils, then regroup to join the adjacent kinomere.

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Human Adenovirus Uptake by Preirnplantation Mouse Embryos

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094607 ◽

1972 ◽

Vol 30 ◽

pp. 268-269

Author(s):

D. G. Chase ◽

W. Winters ◽

L. Piko

Keyword(s):

Hela Cells ◽

Human Adenovirus ◽

Mouse Embryos ◽

Equilibrium Density ◽

Cell Stage ◽

Virus Attachment ◽

Preimplantation Mouse Embryos ◽

Embryo Culture Medium ◽

Preimplantation Mouse ◽

Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

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