Distribution of recombinant VP28 protein in tissues and its immunomodulatory effect against white spot syndrome virus in whiteleg shrimp, Litopenaeus vannamei (Boone, 1931)

2017 ◽  
Vol 25 (5) ◽  
pp. 1761-1776 ◽  
Author(s):  
G. Taju ◽  
N. Madan ◽  
S. Abdul Majeed ◽  
T. Rajkumar ◽  
M. A. Farook ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
Immunomodulatory Effect ◽  
White Spot ◽  
White Spot Syndrome ◽  
Whiteleg Shrimp

scholarly journals Phycoerythrin from Colaconema sp. Has Immunostimulatory Effects on the Whiteleg Shrimp Litopenaeus vannamei and Increases Resistance to Vibrio parahaemolyticus and White Spot Syndrome Virus

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2371
Author(s):  
Po-Tsang Lee ◽  
Jing Huang ◽  
Chin-Yi Huang ◽  
Zi-Xuan Liu ◽  
Han-Yang Yeh ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
White Spot Syndrome Virus ◽  
Expression Profiles ◽  
White Spot ◽  
Shrimp Culture ◽  
White Spot Syndrome ◽  
Whiteleg Shrimp

We investigated whether phycoerythrin (PE), a pigment sourced from marine algae, could act as an immunomodulatory agent in whiteleg shrimp (Litopenaeus vannamei). To this end, PE was extracted and purified from a PE-rich macroalgae, Colaconema sp. Our in vitro analysis demonstrated that PE enhanced prophenoloxidase and phagocytosis activity but inhibited the production of reactive oxygen species in hemocytes. Additionally, the PE signal could be detected using an in vivo imaging system after its injection into the ventral sinus of the cephalothorax of whiteleg shrimp. The expression profiles of fourteen immune-related genes were monitored in hemocytes from whiteleg shrimp injected with 0.30 μg of PE per gram of body weight, and crustin, lysozyme, penaiedin 4, and anti-lipopolysaccharide factor showed up-regulated post-stimulation. The induction of immune genes and enhancement of innate immune parameters by PE may explain the higher survival rates for shrimp that received different doses of PE prior to being challenged with Vibrio parahaemolyticus or white spot syndrome virus compared to controls. Combined, these results show that PE from Colaconema sp. can differentially stimulate the immune response of whiteleg shrimp in vitro and in vivo and could potentially be used as an immunomodulator in shrimp culture.

Influence of stocking density and exposure to white spot syndrome virus in biological performance, metabolic, immune, and bioenergetics response of whiteleg shrimp Litopenaeus vannamei

Aquaculture ◽  
2017 ◽  
Vol 479 ◽  
pp. 528-537 ◽  
Author(s):  
Juan P. Apún-Molina ◽  
Arlett Robles-Romo ◽  
Pindaro Alvarez-Ruiz ◽  
Apolinar Santamaria-Miranda ◽  
Olivia Arjona ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
Stocking Density ◽  
White Spot ◽  
White Spot Syndrome ◽  
Whiteleg Shrimp ◽  
Biological Performance

scholarly journals Small Molecule Inhibitors of White Spot Syndrome Virus: Promise in Shrimp Seedling Culture

2021 ◽  
Vol 22 (7) ◽  
pp. 3450
Author(s):  
Lei Liu ◽  
Li-Peng Shan ◽  
Yan Zhou ◽  
Jiong Chen
Keyword(s):  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
Environmental Issues ◽  
Therapeutic Strategies ◽  
Effective Concentration ◽  
White Spot ◽  
Rapid Production ◽  
White Spot Syndrome ◽  
Median Effective Concentration ◽  
Artificial Pressure

Rapid production of prawn (Litopenaeus vannamei) under artificial pressure can result in a series of obvious challenges and is vulnerable to serious losses related to aquatic environmental issues and the unrestrained outbreak of white spot syndrome (WSS). However, to date, there are no therapeutic strategies to contain the spread of the virus. Here, we synthesized 27 coumarin deriv-atives and evaluated their anti-white spot syndrome virus (WSSV) activity in L. vannamei larvae. We demonstrated that electron-withdrawing and electron-giving substituent groups play an im-portant role in reducing toxicity and WSSV replication, respectively. Two coumarin C2 (2-amino-5-oxo-4-(p-tolyl)-4H,5H-pyrano[3,2-c]chromene-3-carbonitrile) and C7 (2-amino-4-(4-chlorophenyl)-5-oxo-4H,5H-pyrano[3,2-c]chromene-3-carbonitrile) were regarded as the most promising anti-WSSV compounds with maximum antiviral response <5% and median effective concentration <10 mg/L. The mortality of WSSV-infected larvae decreased by more than 60% after exposure to C2 and C7. With continuous immersion of C2 and C7 exchange, the mortality further decreased to 40% at 120 h.Additionally, C2 and C7 are the relatively stable in aquacultural water, making these agents

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