Rapid and cost-effective screening of CRISPR/Cas9-induced mutants by DNA-guided Argonaute nuclease

The issue of whether to screen individuals for Barrett’s esophagus (BE) to prevent esophageal adenocarcinoma (EAC) is highly controversial. Important considerations are that BE is not highly prevalent in the general population and that not many patients with BE develop or die from EAC. Studies that suggest an improved prognosis from surveillance programs are susceptible to lead-time bias. Most of the principles for effective screening, as outlined by the World Health Organization, are not met by endoscopic screening and surveillance protocols. The diagnosis of BE (and dysplasia) is often unclear. Most patients with BE are not identified by screening, and few deaths would be prevented by surveillance. A decision analysis found that the most cost effective screening protocol would be every five years, but the costs associated with prolongation of life are very high, even if a group at high risk for EAC could be identified.

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Restricting HFE genotyping to patients with a transferrin saturation >60% is a cost effective screening strategy in liver clinic patients

Journal of Hepatology ◽

10.1016/s0168-8278(00)80841-7 ◽

2000 ◽

Vol 32 ◽

pp. 133

Author(s):

S. Moodie ◽

L. Ang ◽

G.E. Levin ◽

J. Stenner ◽

V. Murday ◽

...

Keyword(s):

Transferrin Saturation ◽

Cost Effective ◽

Screening Strategy ◽

Effective Screening

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Cost-effective screening strategies for colorectal cancer

Journal of Public Health ◽

10.1093/oxfordjournals.pubmed.a042687 ◽

1992 ◽

Keyword(s):

Colorectal Cancer ◽

Cost Effective ◽

Effective Screening ◽

Screening Strategies

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Cost Effective Screening of Mine Waters Using Accessible Field Test Kits—Experience with a High School Project in the Wonderfonteinspruit Catchment, South Africa

Uranium - Past and Future Challenges ◽

10.1007/978-3-319-11059-2_64 ◽

2014 ◽

pp. 565-572

Author(s):

Lindsay Fyffe ◽

Henk Coetzee ◽

Christian Wolkersdorfer

Keyword(s):

South Africa ◽

High School ◽

Field Test ◽

Cost Effective ◽

Mine Waters ◽

Effective Screening ◽

School Project

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Development of a Dot-Immunoenzyme Filtration Assay for the Detection of Enterovirus 71 Antigen in Human Serum via Immunoglobulin M

Journal of International Medical Research ◽

10.1177/147323001204000332 ◽

2012 ◽

Vol 40 (3) ◽

pp. 1122-1129

Author(s):

A Zhang ◽

M Zhang ◽

H Zhang ◽

H Li ◽

Q Liu

Keyword(s):

Human Serum ◽

Healthy Adult ◽

Enterovirus 71 ◽

Foot And Mouth Disease ◽

Cost Effective ◽

Immunoglobulin M ◽

Healthy Controls ◽

Structural Protein ◽

Serum Samples ◽

Effective Screening

OBJECTIVES: This study evaluated the sensitivity and specificity of a rapid, sensitive dot-immunoenzyme filtration assay to detect enterovirus 71 (EV71) antigen in serum samples from paediatric patients with hand, foot and mouth disease (HFMD), through detection of anti-EV71 immunoglobulin (Ig)M. METHODS: Serum samples from HFMD patients and healthy adult controls were evaluated for the presence of anti-EV71 IgM using a dot-immunoenzyme filtration assay (DIEFA). The results were compared with those obtained using a dot-immunogold filtration assay (DIGFA). The EV71 structural protein VP1 was used as the antigen for both assays. RESULTS: Serum samples from 72 HFMD patients and 54 healthy controls were evaluated. The DIGFA procedure showed a sensitivity of 98.5% and a specificity of 100%, whereas the DIEFA procedure showed a sensitivity of 98.6% and a specificity of 98.0%. There were no significant differences between the assays in either specificity or sensitivity. CONCLUSION: The DIEFA procedure developed in this study has potential as a rapid, simple, sensitive and cost-effective screening technique for detecting EV71 antigen in serum samples from patients with HFMD.

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