Regional Comparison of Snow Leopard (Panthera uncia) Diet using DNA Metabarcoding

2021 ◽  
Vol 30 (3) ◽  
pp. 797-817
Author(s):  
Charlotte E. Hacker ◽  
Matthew Jevit ◽  
Shafqat Hussain ◽  
Ghulam Muhammad ◽  
Bariushaa Munkhtsog ◽  
...  
Keyword(s):  
Snow Leopard ◽  
Regional Comparison ◽  
Dna Metabarcoding ◽  
Panthera Uncia

Use of equine amniotic membrane free-island grafts for treatment of a midstromal corneal ulcer and descemetocele in a snow leopard (Panthera uncia)

2018 ◽  
Vol 253 (12) ◽  
pp. 1623-1629
Author(s):  
Amy M. Knollinger ◽  
Jessica E. McDonald ◽  
Nancy A. Carpenter ◽  
Erika K. Crook
Keyword(s):  
Amniotic Membrane ◽  
Corneal Ulcer ◽  
Snow Leopard ◽  
Panthera Uncia

scholarly journals Prey Preferences of the Snow Leopard (Panthera uncia): Regional Diet Specificity Holds Global Significance for Conservation

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e88349 ◽  
Author(s):  
Salvador Lyngdoh ◽  
Shivam Shrotriya ◽  
Surendra P. Goyal ◽  
Hayley Clements ◽  
Matthew W. Hayward ◽  
...  
Keyword(s):  
Snow Leopard ◽  
Prey Preferences ◽  
Panthera Uncia

scholarly journals Snow Leopard Dietary Preferences and Livestock Predation Revealed by Fecal DNA Metabarcoding: No Evidence for Apparent Competition Between Wild and Domestic Prey

2021 ◽  
Vol 9 ◽  
Author(s):  
Qi Lu ◽  
Lingyun Xiao ◽  
Chen Cheng ◽  
Zhi Lu ◽  
Jindong Zhao ◽  
...  
Keyword(s):  
Dietary Habits ◽  
Negative Relationship ◽  
Apparent Competition ◽  
Mitigation Strategies ◽  
Snow Leopard ◽  
Livestock Predation ◽  
Prey Preferences ◽  
Livestock Density ◽  
Dna Metabarcoding ◽  
Snow Leopards

Accurate assessments of the patterns and drivers of livestock depredation by wild carnivores are vital for designing effective mitigation strategies to reduce human-wildlife conflict. Snow leopard’s (Panthera uncia) range extensively overlaps pastoralist land-use and livestock predation there is widely reported, but the ecological determinants of livestock consumption by snow leopards remain obscure. We investigated snow leopard dietary habits at seven sites across the Sanjiangyuan region of the Qinghai–Tibetan Plateau (QTP), an area central to the species’ global range. Snow leopard abundance, wild prey composition, and livestock density varied among those sites, thus allowing us to test the effects of various factors on snow leopard diet and livestock predation. Using DNA metabarcoding, we obtained highly resolved dietary data from 351 genetically verified snow leopard fecal samples. We then analyzed the prey preferences of snow leopards and examined ecological factors related to their livestock consumption. Across the sites, snow leopard prey was composed mainly of wild ungulates (mean = 81.5% of dietary sequences), particularly bharal (Pseudois nayaur), and supplemented with livestock (7.62%) and smaller mammals (marmots, pikas, mice; 10.7%). Snow leopards showed a strong preference for bharal, relative to livestock, based on their densities. Interestingly, both proportional and total livestock consumption by snow leopards increased linearly with local livestock biomass, but not with livestock density. That, together with a slight negative relationship with bharal density, supports apparent facilitation between wild and domestic prey. We also found a significant positive correlation between population densities of snow leopard and bharal, yet those densities showed slight negative relationships with livestock density. Our results highlight the importance of sufficient wild ungulate abundance to the conservation of viable snow leopard populations. Additionally, livestock protection is critically needed to reduce losses to snow leopard depredation, especially where local livestock abundances are high.

scholarly journals Prey Preference of Snow Leopard (Panthera uncia) in South Gobi, Mongolia

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e32104 ◽  
Author(s):  
Wasim Shehzad ◽  
Thomas Michael McCarthy ◽  
Francois Pompanon ◽  
Lkhagvajav Purevjav ◽  
Eric Coissac ◽  
...  
Keyword(s):  
Prey Preference ◽  
Snow Leopard ◽  
Panthera Uncia

scholarly journals Snow Leopard (Panthera uncia ) Spermatozoa Are Sensitive to Alkaline pH, But Motility In Vitro Is Not Influenced by Protein or Energy Supplements

1996 ◽  
Vol 17 (5) ◽  
pp. 558-566 ◽  
Author(s):  
Terri L. Roth ◽  
William F. Swanson ◽  
Darin Collins ◽  
Michael Burton ◽  
Della M. Garell ◽  
...  
Keyword(s):  
Snow Leopard ◽  
Alkaline Ph ◽  
Energy Supplements ◽  
Panthera Uncia

Current Status of the Eastern Sayan Snow Leopard (Panthera uncia) Grouping and Its Nutritive Base

2018 ◽  
Vol 45 (9) ◽  
pp. 1106-1115
Author(s):  
A. S. Karnaukhov ◽  
S. V. Malykh ◽  
M. P. Korablev ◽  
Yu. M. Kalashnikova ◽  
A. D. Poyarkov ◽  
...  
Keyword(s):  
Current Status ◽  
Snow Leopard ◽  
Eastern Sayan ◽  
Panthera Uncia

Inducing pluripotency in somatic cells from the snow leopard (Panthera uncia), an endangered felid

2012 ◽  
Vol 77 (1) ◽  
pp. 220-228.e2 ◽  
Author(s):  
R. Verma ◽  
M.K. Holland ◽  
P. Temple-Smith ◽  
P.J. Verma
Keyword(s):  
Somatic Cells ◽  
Snow Leopard ◽  
Panthera Uncia

118 INDUCING PLURIPOTENCY IN SOMATIC CELLS FROM THE SNOW LEOPARD (PANTHERA UNCIA), AN ENDANGERED FELID

2012 ◽  
Vol 24 (1) ◽  
pp. 171
Author(s):  
R. Verma ◽  
M. Holland ◽  
P. Smith ◽  
P. Verma
Keyword(s):  
Fluorescent Protein ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Retroviral Vectors ◽  
Ips Cells ◽  
Transduction Efficiency ◽  
Snow Leopard ◽  
Ips Cell ◽  
Induced Pluripotency ◽  
Panthera Uncia

Induced pluripotency is a new approach to produce embryonic stem-like cells from somatic cells that provides a unique means to understand both pluripotency and lineage assignment. To investigate whether this technology could be applied to endangered species, where the limited availability of gametes makes research on embryonic stem cells difficult, we attempted generation of induced pluripotent stem (iPS) cells from snow leopard (Panthera uncia) fibroblasts by retroviral transfection with Moloney-based retroviral vectors (pMX) encoding either 4 (Oct-4, Sox-2, Klf-4 and cMyc) or 5 (Oct-4, Sox-2, Klf-4, cMyc and Nanog) human transcription factors. Transduction efficiency of the retrovirus was ascertained using pMX-green fluorescent protein transgene expression and averaged 96% from 3 repeated experiments. The reprogramming efficiency of initial colony formation was 0.000308% (37/120 000 cells plated) for 4-factor induction compared with 0.000517% (62/120 000) for 5-factor induction. Transduction with 4 factors resulted in the formation of small colonies of cells, which could not be maintained for more than 4 passages (P4). However, addition of Nanog to the transfection cocktail produced stable iPS cell colonies, which formed as early as Day 3. Colonies of cells were selected at Day 5 and expanded in vitro on mouse embryonic fibroblast feeder cells. The resulting cell line was positive for alkaline phosphatase, Oct-4, Nanog and stage-specific embryonic antigen-4 at P14. Also, RT-PCR confirmed that endogenous Oct-4 and Nanog were expressed by snow leopard iPS cells from P4; although all 5 human transgenes were transcribed at P4, Oct-4, Sox-2 and Nanog transgenes were silenced as early as P14, suggesting that reprogramming of the endogenous pluripotent genes had occurred. When injected into immune-deficient mice, snow leopard iPS cells formed teratomas containing tissues representative of the 3 germ layers. This study describes the first derivation of iPS cells from the endangered snow leopard and is also the first report on induced pluripotency in felid species. Our results demonstrate that addition of Nanog to the reprogramming cocktail was essential for derivation of iPS lines in this felid and that iPS cells provide a unique source of pluripotent cells with utility in conservation for cryopreservation of genetics, as a source of reprogrammed donor cells for nuclear transfer or for directed differentiation to gametes in the future.

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