Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil

Interactions among jasmonates and auxin in the photoperiodic flower induction of a short-day plant Ipomoea nil were examined. Therefore, we measured changes in jasmonic acid (JA) and jasmonic acid methyl ester (JAMe) levels in the cotyledons of I. nil during the inductive night, as well as the effects of indole-3-acetic acid (IAA) on their content. We noticed an interesting result, that IAA applied on the cotyledons of I. nil is an effective stimulator of JAMe production in seedlings cultivated under inductive night conditions. IAA treatment also significantly increased the transcriptional activity of InJMT (JASMONIC ACID CARBOXYL METHYLTRANSFERASE), while did not affect the expression of JA biosynthesis genes (lipoxyganease, allene oxide synthase, 12-oxophytodienoate reductase). These data, as well as the results of our previous research, suggest that exogenous IAA participates in I. nil flower induction process by stimulating InJMT expression and, as a consequence of that, enhancing the level of JAMe, a flowering inhibitor.

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A new MFS-transporter gene next to the gibberellin biosynthesis gene cluster of Gibberella fujikuroi is not involved in gibberellin secretion

Current Genetics ◽

10.1007/s002940100218 ◽

2001 ◽

Vol 39 (5-6) ◽

pp. 377-383 ◽

Cited By ~ 8

Author(s):

Thorsten Vo� ◽

Jessica Schulte ◽

Bettina Tudzynski

Keyword(s):

Gene Cluster ◽

Gibberella Fujikuroi ◽

Gibberellin Biosynthesis ◽

Transporter Gene ◽

Biosynthesis Gene Cluster ◽

Biosynthesis Gene ◽

Mfs Transporter

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Class I TCP Transcription Factors Target the Gibberellin Biosynthesis Gene GA20ox1 and the Growth-Promoting Genes HBI1 and PRE6 during Thermomorphogenic Growth in Arabidopsis

Plant and Cell Physiology ◽

10.1093/pcp/pcz137 ◽

2019 ◽

Vol 60 (8) ◽

pp. 1633-1645 ◽

Cited By ~ 7

Author(s):

Lucía V Ferrero ◽

Ivana L Viola ◽

Federico D Ariel ◽

Daniel H Gonzalez

Keyword(s):

Transcription Factors ◽

Ambient Temperature ◽

Cell Expansion ◽

High Ambient Temperature ◽

Class I ◽

Gibberellin Biosynthesis ◽

Binding Motifs ◽

Growth Defects ◽

Biosynthesis Gene ◽

Growth Promoting

Abstract Plants respond to a rise in ambient temperature by increasing the growth of petioles and hypocotyls. In this work, we show that Arabidopsis thaliana class I TEOSINTE BRANCHED 1, CYCLOIDEA, PCF (TCP) transcription factors TCP14 and TCP15 are required for optimal petiole and hypocotyl elongation under high ambient temperature. These TCPs influence the levels of the DELLA protein RGA and the expression of growth-related genes, which are induced in response to an increase in temperature. However, the class I TCPs are not required for the induction of the auxin biosynthesis gene YUCCA8 or for auxin-dependent gene expression responses. TCP15 directly targets the gibberellin biosynthesis gene GA20ox1 and the growth regulatory genes HBI1 and PRE6. Several of the genes regulated by TCP15 are also targets of the growth regulator PIF4 and show an enrichment of PIF4- and TCP-binding motifs in their promoters. PIF4 binding to GA20ox1 and HBI1 is enhanced in the presence of the TCPs, indicating that TCP14 and TCP15 directly participate in the induction of genes involved in gibberellin biosynthesis and cell expansion by high temperature functionally interacting with PIF4. In addition, overexpression of HBI1 rescues the growth defects of tcp14 tcp15 double mutants, suggesting that this gene is a major outcome of regulation by both class I TCPs during thermomorphogenesis.

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