Revealing the high-resolution three-dimensional network of chromatin and interchromatin space: A novel electron-microscopic approach to reconstructing nuclear architecture

2009 ◽  
Vol 17 (6) ◽  
pp. 801-810 ◽  
Author(s):  
Jacques Rouquette ◽  
Christel Genoud ◽  
Gerardo H. Vazquez-Nin ◽  
Bernd Kraus ◽  
Thomas Cremer ◽  
...  
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Nuclear Architecture ◽  
Microscopic Approach ◽  
Electron Microscopic ◽  
Dimensional Network ◽  
Interchromatin Space

High-resolution transmission electron microscopic investigations of molybdenum thin films on faceted α-Al2O3

2005 ◽  
Vol 38 (2) ◽  
pp. 260-265 ◽  
Author(s):  
Leonore Wiehl ◽  
Jens Oster ◽  
Michael Huth
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Epitaxial Relationship ◽  
Electron Microscopic ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
High Resolution Images ◽  
Α Al2o3 ◽  
Relationship Of

Epitaxially grown Mo films on a faceted corundum (α-Al2O3)mplane were investigated by transmission electron microscopy. Low- and high-resolution images were taken from a cross-section specimen cut perpendicular to the facets. It was possible to identify unambiguously the crystallographic orientation of these facets and explain the considerable deviation (∼10°) of the experimental interfacet angle, as measured with atomic force microscopy (AFM), from the expected value. For the first time, proof is given for a smooth \{10\bar{1}1\} facet and a curvy facet with orientation near to \{10\bar{1}\bar{2}\}. Moreover, the three-dimensional epitaxial relationship of an Mo film on a faceted corundummsurface was determined.

Effect of acoustic overstimulation on the glycocalyx and the ciliary interconnections in the organ of Corti: High resolution scanning electron microscopic investigation

1989 ◽  
Vol 103 (12) ◽  
pp. 1125-1129 ◽  
Author(s):  
M. Takumida ◽  
L. Fredelius ◽  
D. Bagger-Sjöbäck ◽  
Y. Harada ◽  
J. Wersäll
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Organ Of Corti ◽  
Staining Technique ◽  
Microscopic Investigation ◽  
Electron Microscopic Investigation ◽  
Outer Hair Cells ◽  
Electron Microscopic ◽  
Acoustic Overstimulation ◽  
Scanning Electron

AbstractChanges in ciliary interconnections in the organ of Corti are described after acoustic overstimulation using a special high resolution scanning electron microscope and tannic acid-osmium staining technique, giving an almost three dimensional view. Guinea pigs were exposed to a 3.85 kHz pure tone at an intensity of 120 dB for 22.5 minutes. The first detectable change was a disarrangement of the cilia with a loosening of the interconnections. The ciliary plasma membrane presented with an abnormally smooth appearance. The tip links connecting the tips of the stereocilia to their taller neighbours were also affected showing elongation or even disappearance. The fine granules which cover the tips of the tallest stereocilia of the outer hair cells were decreased. These findings suggest that acoustic overstimulation may affect the carbohydrate metabolism exceding to degeneration of ciliary interconnections resulting in a disarrangement and detachment of cilia. The tip links, which may participate in sensory cell transduction, seem also to be affected by acoustic overstimulation.

The Structure of the System: Filler Rubber. An Electron-Microscopic Study

1954 ◽  
Vol 27 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Friedrich Endter
Keyword(s):  
Natural Rubber ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Three Dimensional ◽  
Electron Microscopic ◽  
Rubber Mixture ◽  
Dimensional Network ◽  
Bound Rubber ◽  
Microscopic Investigations

Abstract Electron microscopic investigations of the benzene-extraction residues of filler-natural rubber mixtures which are used for the determination of bound rubber, give a pattern of a three-dimensional network consisting of filler and bound rubber. The unchanged benzene-soluble rubber is present in the interstices of the network in the untreated filler-rubber mixture.

scholarly journals Fluorescence photooxidation with eosin: a method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy.

1994 ◽  
Vol 126 (4) ◽  
pp. 901-910 ◽  
Author(s):  
T J Deerinck ◽  
M E Martone ◽  
V Lev-Ram ◽  
D P Green ◽  
R Y Tsien ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Light And Electron Microscopy ◽  
Three Dimensional ◽  
Substantial Improvement ◽  
Electron Microscopic ◽  
Simple Method ◽  
High Voltage Electron Microscopy

A simple method is described for high-resolution light and electron microscopic immunolocalization of proteins in cells and tissues by immunofluorescence and subsequent photooxidation of diaminobenzidine tetrahydrochloride into an insoluble osmiophilic polymer. By using eosin as the fluorescent marker, a substantial improvement in sensitivity is achieved in the photooxidation process over other conventional fluorescent compounds. The technique allows for precise correlative immunolocalization studies on the same sample using fluorescence, transmitted light and electron microscopy. Furthermore, because eosin is smaller in size than other conventional markers, this method results in improved penetration of labeling reagents compared to gold or enzyme based procedures. The improved penetration allows for three-dimensional immunolocalization using high voltage electron microscopy. Fluorescence photooxidation can also be used for high resolution light and electron microscopic localization of specific nucleic acid sequences by in situ hybridization utilizing biotinylated probes followed by an eosin-streptavidin conjugate.

Cytoskeletal control of neuronal geometry: A serial EM analysis

1988 ◽  
Vol 46 ◽  
pp. 24-25
Author(s):  
John K. Stevens ◽  
Judy Trogadis
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Three Dimensional ◽  
Video Camera ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Direct Role ◽  
Live Image ◽  
Neuronal Geometry ◽  
Rotation Stage

The cytoskeleton plays a direct role in controlling neurite shape. To quantitatively study both the three dimensional shape and the sub-micron structure of the cytoskeleton requires complete serial reconstruction at the Electron Microscopic level. We have devised a computer reconstruction system specifically for this purpose.The system uses a 35mm film copy of 3.25 x 4.00 inch EM negative as the data source. The film is placed into a high speed film transport (15 frames/second), which is mounted on a X,Y and rotation stage controlled by stepping motors. The 35mm film is viewed through a stepping motor controlled zoom lens mounted on a high resolution (1119 x 1024) video camera. A high resolution frame grabber controlled by the computer can store one complete frame. Thus, the live image and a stored image may be displayed alternately on a high resolution monitor. Finally, a graphics overlay and mouse connected to the computer can be used to align successive sections via the stepping motors, as well as to trace the outlines of a profile, or of a microtubule.

scholarly journals Ultrastructure of the adhesion of bacteria to the epithelial cell membrane of three-day postnatal rat tongue mucosa: a transmission and high-resolution scanning electron microscopic study

2007 ◽  
Vol 18 (4) ◽  
pp. 320-323 ◽  
Author(s):  
Ii-sei Watanabe ◽  
Koichi Ogawa ◽  
Marcelo Cavenaghi Pereira da Silva ◽  
Aracy Akiko Motoyama ◽  
Eduardo Shigueaki Kado ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Cell Membrane ◽  
Membrane Surface ◽  
Three Dimensional ◽  
Electron Microscopic ◽  
Tem Analysis ◽  
Native Flora ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron

Togue mucosa surface of 3-day postnatal rats was examined under transmission electron microscopy (TEM) and high-resolution scanning electron microscopy (HRSEM). For HRSEM analysis, the specimens were fixed in the same solution for 24 h, postfixed in 2% osmiun tetroxide, critical-point dried and coated with platinum-palladium. For TEM analysis, the specimens were fixed using modified Karnovsky solution and embedded in Spurr resin. The results revealed the presence of numerous microplicae in the membrane surface of keratinized epithelial cells to which groups of bacteria were attached. These bacteria were staphylococcus and coccus organized either in rows or at random, which were visualized in three-dimensional HRSEM images. At high magnification, the TEM images revealed the adhesion of bacteria to the cell membrane through numerous filamentous structures comprising the glycocalyx. The fine fibrillar structures rising from each bacterium and from cell membrane were clearly seen. These characteristics on bacteria structure may be used for future control or prevention of bacterial diseases and for installation of the oral native flora.

scholarly journals Demonstration of antigens at both sides of plasma membranes in one coincident electron microscopic image: a double-immunogold replica study of virus-infected cells.

1988 ◽  
Vol 36 (8) ◽  
pp. 1015-1021 ◽  
Author(s):  
G Rutter ◽  
W Bohn ◽  
H Hohenberg ◽  
K Mannweiler
Keyword(s):  
High Resolution ◽  
Measles Virus ◽  
Plasma Membranes ◽  
Protein A ◽  
Three Dimensional ◽  
Electron Microscopic ◽  
Cytoplasmic Surface ◽  
Critical Point Drying ◽  
Electron Microscopic Image ◽  
Infected Cells

We present here a procedure for obtaining high-resolution topographical information about the spatial distribution of antigens at both sides of isolated plasma membranes. HeLa cells grown on coverslips and infected with measles virus served as a model system. Virus glycoproteins appearing at the cell surface were demonstrated by tagging them with rabbit anti-measles antibodies and protein A-gold probes. Cells were stabilized with tannic acid, covered with a cationized coverslip, and then split in potassium-containing buffer. Membranes adherent to the cationized coverslip were fixed in formaldehyde-glutaraldehyde and reacted with mouse monoclonal antibodies against various structural proteins of measles virus. Antibody binding sites at the cytoplasmic surface were visualized either by the antibody bridge method, using normal mouse Ig coupled to gold colloid of different sizes, or by the peroxidase-antiperoxidase procedure. After osmication and critical point-drying, the cytoplasmic surfaces were replicated by platinum-carbon evaporation and examined by TEM without prior cleaning from biological material. This new method permits concomitant localization of antigens present at the inner and outer leaflets of the plasma membrane, and provides high-resolution information about the three-dimensional organization of the cytoplasmic surface.

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