Development of Novel Microsatellite Loci and Assessment of Genetic Diversity in the Endangered Crested Ibis, Nipponia Nippon

2006 ◽  
Vol 7 (1) ◽  
pp. 157-160 ◽  
Author(s):  
Lu-Ping He ◽  
Qiu-Hong Wan ◽  
Sheng-Guo Fang ◽  
Yong-Mei Xi
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Nipponia Nippon ◽  
Crested Ibis

scholarly journals Low genetic diversity in the Endangered Crested Ibis Nipponia nippon and implications for conservation

2004 ◽  
Vol 14 (3) ◽  
pp. 183-190 ◽  
Author(s):  
BEI ZHANG ◽  
SHENG-GUO FANG ◽  
YONG-MEI XI
Keyword(s):  
Genetic Diversity ◽  
Control Region ◽  
Haplotype Diversity ◽  
Mtdna Control Region ◽  
Significant Information ◽  
Nipponia Nippon ◽  
Low Genetic Diversity ◽  
Captive Populations ◽  
Crested Ibis ◽  
Wide Range

Crested Ibis Nipponia nippon is an Endangered species that has experienced a severe but short-lived population bottleneck in recent years. Only one wild population and two captive populations exist in China today, all of which were probably initiated from two breeding pairs rediscovered in 1981. This paper represents the first study of the genetic diversity of Crested Ibis by analysing sequence variation of a fragment spanning domains II and III of the mitochondrial DNA (mtDNA) control region of both wild and captive populations. Two haplotypes were identified in both wild and captive populations, and the captive population had a bias towards haplotype 1. The species as a whole demonstrated an extremely low level of genetic diversity, with a haplotype diversity and nucleotide diversity of 0.386 ± 0.074 and 0.069% ± 0.013%, respectively. Despite the rapid increase in numbers of birds under intensive management, the paucity of genetic diversity remains a real threat to the species. The mtDNA control region variations detected in the present study could provide significant information additional to the studbook data of Crested Ibis; therefore we suggest that special attention be paid to individuals with haplotype 2 when considering captive breeding management. Overall, great care should be taken in the selection of reintroduction sites for this rare bird, as species with low genetic diversity are thought to be more limited in their ability to tolerate a wide range of environmental extremes and diseases.

Assessment of the genetic diversity of microsatellite loci of horses of Heavy Draft breeds

2018 ◽  
pp. 39-44
Author(s):  
N. Blohina ◽  
◽  
L. Khrabrova ◽  
A. Zaitcev ◽  
I. Gavrilicheva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci

Crested Ibis (Nipponia nippon)

2020 ◽  
Author(s):  
Eloïsa Matheu ◽  
Josep del Hoyo ◽  
Guy M. Kirwan ◽  
Ernest Garcia
Keyword(s):  
Nipponia Nippon ◽  
Crested Ibis

scholarly journals Stand out from the Crowd: Small-Scale Genetic Structuring in the Endemic Sicilian Pond Turtle

Diversity ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 343
Author(s):  
Luca Vecchioni ◽  
Federico Marrone ◽  
Marco Arculeo ◽  
Uwe Fritz ◽  
Melita Vamberger
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Small Scale ◽  
Moderate Degree ◽  
Geographical Pattern ◽  
Genetic Structuring ◽  
Pond Turtle ◽  
Emys Trinacris ◽  
Polymorphic Microsatellite ◽  
Entire Distribution

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

scholarly journals Genetic diversity and population structure of Plasmodium falciparum in Nigeria: insights from microsatellite loci analysis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Fehintola V. Ajogbasile ◽  
Adeyemi T. Kayode ◽  
Paul E. Oluniyi ◽  
Kazeem O. Akano ◽  
Jessica N. Uwanibe ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Population Structure ◽  
Microsatellite Loci ◽  
Control Strategies ◽  
Population Diversity ◽  
Parasite Population ◽  
Nested Polymerase Chain Reaction ◽  
Public Health Burden ◽  
Genetic Diversity And Structure

Abstract Background Malaria remains a public health burden especially in Nigeria. To develop new malaria control and elimination strategies or refine existing ones, understanding parasite population diversity and transmission patterns is crucial. Methods In this study, characterization of the parasite diversity and structure of Plasmodium falciparum isolates from 633 dried blood spot samples in Nigeria was carried out using 12 microsatellite loci of P. falciparum. These microsatellite loci were amplified via semi-nested polymerase chain reaction (PCR) and fragments were analysed using population genetic tools. Results Estimates of parasite genetic diversity, such as mean number of different alleles (13.52), effective alleles (7.13), allelic richness (11.15) and expected heterozygosity (0.804), were high. Overall linkage disequilibrium was weak (0.006, P < 0.001). Parasite population structure was low (Fst: 0.008–0.105, AMOVA: 0.039). Conclusion The high level of parasite genetic diversity and low population structuring in this study suggests that parasite populations circulating in Nigeria are homogenous. However, higher resolution methods, such as the 24 SNP barcode and whole genome sequencing, may capture more specific parasite genetic signatures circulating in the country. The results obtained can be used as a baseline for parasite genetic diversity and structure, aiding in the formulation of appropriate therapeutic and control strategies in Nigeria.

scholarly journals Isolation and Characterization of Microsatellite Loci of Clarias macrocephalus and Their Application to Genetic Diversity Study

1999 ◽  
Vol 65 (4) ◽  
pp. 520-526 ◽  
Author(s):  
Uthairat Na-Nakorn ◽  
Nobuhiko Taniguchi ◽  
Estu Nugroho ◽  
Shingo Seki ◽  
Wongpathom Kamonrat
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Isolation And Characterization ◽  
Clarias Macrocephalus ◽  
Genetic Diversity Study ◽  
Diversity Study

scholarly journals Breeding ecology and success of a reintroduced population of the endangered Crested Ibis Nipponia nippon

2014 ◽  
Vol 25 (2) ◽  
pp. 207-219 ◽  
Author(s):  
XIAOPING YU ◽  
XIA LI ◽  
ZHIPING HUO
Keyword(s):  
Endangered Species ◽  
Radio Telemetry ◽  
Natural Habitat ◽  
Breeding Ecology ◽  
Active Nest ◽  
Nipponia Nippon ◽  
Crested Ibis ◽  
South Slope ◽  
Reintroduced Population ◽  
Elaphe Carinata

SummaryReintroduction projects usually attempt to re-establish a self-sustaining population of endangered species within their historical ranges through the release of captive-bred individuals into the wild. We studied the breeding biology and nesting success of a reintroduced population of Crested Ibis Nipponia nippon in Ningshan County on the south slope of the Qinling Mountains of Shaannxi Province, China. From May 2007 to October 2011, 56 captive-bred individuals, composed of 26 females and 30 males, were released into the area and monitored using radio-telemetry. The average age of individuals at release was 5.2 ± 2.5 years for females and 6.4 ± 2.9 years for males. Mean clutch size was 3.14±1.06 (range 1-5). Mean number of fledglings per active nest was 1.57 ± 1.03 and mean number of fledglings per successful nest was 2.00 ± 0.87. Most of the fledglings survived to complete their post-fledging dispersal. Mean annual survival rate was 0.552 ± 0.064 for all released birds, 0.815 ± 0.054 for breeders, and 0.515 ± 0.058 for first year fledglings. The average number of young produced/year/pair was 1.57 ± 1.03 and the growth rate (λ) of the reintroduced population was 1.2193. We confirmed that starvation and predation by King Rat Snake Elaphe carinata were the main causes of death of released individuals, nestlings and fledglings. This is the first attempt to reintroduce captive-bred Crested Ibis to former natural habitat. The ability of captive-bred ibis to survive and breed successfully bodes well for future releases of this and other endangered species.

The spatial genetic structure of bank vole (Myodes glareolus) and yellow-necked mouse (Apodemus flavicollis) populations: The effect of distance and habitat barriers

2009 ◽  
Vol 59 (2) ◽  
pp. 169-187 ◽  
Author(s):  
Michal Kozakiewicz ◽  
Alicja Gryczyńska–Siemiątkowska ◽  
Hanna Panagiotopoulou ◽  
Anna Kozakiewicz ◽  
Robert Rutkowski ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Bank Vole ◽  
Microsatellite Loci ◽  
Myodes Glareolus ◽  
Apodemus Flavicollis ◽  
Island Populations ◽  
Local Populations ◽  
Bank Vole Myodes Glareolus ◽  
Yellow Necked Mouse

AbstractHabitat barriers are considered to be an important factor causing the local reduction of genetic diversity by dividing a population into smaller sections and preventing gene flow between them. However, the “barrier effect” might be different in the case of different species. The effect of geographic distance and water barriers on the genetic structure of populations of two common rodent species – the yellow-necked mouse (Apodemus flavicollis) and the bank vole (Myodes glareolus) living in the area of a lake (on its islands and on two opposite shores) was investigated with the use of microsatellite fragment analysis. The two studied species are characterised by similar habitat requirements, but differ with regard to the socio-spatial structure of the population, individual mobility, capability to cross environmental barriers, and other factors. Trapping was performed for two years in spring and autumn in north-eastern Poland (21°E, 53°N). A total of 160 yellow-necked mouse individuals (7 microsatellite loci) and 346 bank vole individuals (9 microsatellite loci) were analysed. The results of the differentiation analyses (FST and RST) have shown that both the barrier which is formed by a ca. 300 m wide belt of water (between the island and the mainland) and the actual distance of approximately 10 km in continuous populations are sufficient to create genetic differentiation within both species. The differences between local populations living on opposite lake shores are the smallest; differences between any one of them and the island populations are more distinct. All of the genetic diversity indices (the mean number of alleles, mean allelic richness, as well as the observed and expected heterozygosity) of the local populations from the lakeshores were significantly higher than of the small island populations of these two species separated by the water barrier. The more profound “isolation effect” in the case of the island populations of the bank vole, in comparison to the yellow-necked mouse populations, seems to result not only from the lower mobility of the bank vole species, but may also be attributed to other differences in the animals' behaviour.

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