Structural Characteristics and Antitumor Activity of Fucoidans from the Brown Alga Sargassum muticum

A water-soluble polysaccharide named as HPS-1 was isolated from the roots of Hedysarum polybotrys Hand.-Mazz by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its antitumor activity. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, GLCMS, methylation analysis, Periodate oxidation and Smith degradation. Based on the data obtained, HPS-1 was found to be an α- (14)-D-glucan, with a single α-D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 9.4×104 Da. MTT assay revealed that HPS-1 significantly inhibited the proliferation of Human hepatocellular carcinoma HEP-G2 cells and human gastric cancer MGC-803 cells in vitro, indicating HPS-1 could have a possible cancer therapeutic potential.

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Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-018-02936-5 ◽

2018 ◽

Vol 188 (2) ◽

pp. 481-490 ◽

Cited By ~ 6

Author(s):

Hai-yu Ji ◽

Pei Chen ◽

Juan Yu ◽

Ying-ying Feng ◽

An-jun Liu

Keyword(s):

Heat Treatment ◽

Antitumor Activity ◽

Structural Characteristics ◽

Grifola Frondosa

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Canopy characteristics of the brown alga Sargassum muticum (Fucales, Phaeophyta) in Lake Grevelingen, southwest Netherlands

Hydrobiologia ◽

10.1007/bf00040236 ◽

1990 ◽

Vol 204-205 (1) ◽

pp. 211-217 ◽

Cited By ~ 21

Author(s):

A. T. Critchley ◽

P. R. M. De Visscher ◽

P. H. Nienhuis

Keyword(s):

Brown Alga ◽

Sargassum Muticum ◽

Lake Grevelingen

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Enemy release an unlikely explanation for the invasive potential of the brown alga Sargassum muticum: experimental results, literature review and meta-analysis

Marine Biology ◽

10.1007/s00227-016-2968-x ◽

2016 ◽

Vol 163 (10) ◽

Cited By ~ 4

Author(s):

Morten Foldager Pedersen ◽

Kathrine L. Johnsen ◽

Louise L. Halle ◽

Nadja D. Karling ◽

Tiina Salo

Keyword(s):

Literature Review ◽

Brown Alga ◽

Meta Analysis ◽

Sargassum Muticum ◽

Experimental Results ◽

Enemy Release ◽

Invasive Potential

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Immunoadjuvant Activity of Fucoidans from the Brown Alga Fucus evanescens

Marine Drugs ◽

10.3390/md18030155 ◽

2020 ◽

Vol 18 (3) ◽

pp. 155 ◽

Cited By ~ 4

Author(s):

Tatyana A. Kuznetsova ◽

Tatyana P. Smolina ◽

Ilona D. Makarenkova ◽

Lydmila A. Ivanushko ◽

Elena V. Persiyanova ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Brown Alga ◽

Structural Characteristics ◽

Therapeutic Vaccines ◽

Fucus Evanescens ◽

Structural Modifications ◽

Adjuvant Effects ◽

Antigen Presenting

The study presents the results of a comparative evaluation of the effect of structural modifications of fucoidans from the brown alga Fucus evanescens (native, highly purified product of fucoidan enzymatic hydrolysis, a new regular 1→3;1→4-α-L-fucan, sulphated mainly at C2 and acetylated at C4 of the fucose residue) on the effector functions of innate and adaptive immunity cells in vitro and in vivo. Using flow cytometry, we found that all examined fucoidans induce the maturation of dendritic cells, enhance the ability of neutrophils to migrate and adhere, activate monocytes and enhance their antigen-presenting functions, and increase the cytotoxic potential of natural killers. Fucoidans increase the production of hepatitis B virus (HBs) specific IgG and cytokine Th1 (IFN-γ, TNF-α) and Th2 (IL-4) profiles in vivo. The data obtained suggest that in vitro and in vivo adjuvant effects of the products of fucoidan enzymatic hydrolysis with regular structural characteristics are comparable to those of the native fucoidan. Based on these data, the products of fucoidan enzymatic hydrolysis can be considered as an effective and safe candidate adjuvant to improve the efficacy of prophylactic and therapeutic vaccines.

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