Seasonal patterns of growth, alginate content and block structure of the alien invader Sargassum muticum (Fucales, Ochrophyta) from the Atlantic coast of Morocco

In the present study, alginate yield and composition were investigated during the seasonal life cycle of the alien brown alga Sargassum muticum harvested from the Atlantic coast of Morocco. Alginate yield ranged from 11.14% in winter to 25.62% in spring/early summer, coinciding with maximum vegetative growth. Monthly monitoring of the alginate block structure showed that the highest mannuronate (M)/guluronate (G) ratio was recorded during the maximum development of S. muticum, before sexual maturity and during resumption of vegetative growth, giving maximum flexibility to the alga. The unusually high molar monad fractions (FG) and dyad fractions (FGG) of guluronic acid in late summer/early autumn appeared to be related to stiffness of senescent thalli. Rheological characterisation showed that the alginate of S. muticum exhibited pseudoplastic behaviour, with the highest apparent viscosities measured in late summer/early autumn when the G blocks dominated the alginate structure. This study suggests that S. muticum could be exploited as an alginophyte for commercial applications. The best harvest time is May-June, which corresponds to the highest alginate yield, maximum thallus growth, and largely completed sexual reproduction, ensuring sustainable exploitation of the species.

Physiological Responses of Sargassum muticum, a Potential Golden Tide Species, to Different Levels of Light and Nitrogen

Sargassum golden tides have bloomed frequently in many sea areas throughout the world, and negatively impacted on the local marine ecology. Sargassum muticum commonly inhabits rocky shores. It is now distributed worldwide due to its invasiveness, and recently drifting individuals have been observed on the coasts of Canary Islands. However, as a potential golden tide alga, physiological, and ecological studies of this species have not been frequently explored. To investigate the responses of S. muticum to light and nitrogen, two key environmental factors in golden tide formation, we established three light levels (LL, low light, 10 μmol photons m–2 s–1; ML, medium light, 60 μmol photons m–2 s–1, and HL, high light, 300 μmol photons m–2 s–1) and two nitrogen levels (LN, low nitrogen, 25.0 μM of natural seawater; HN, high nitrogen, 125.0 μM), and cultivated the thalli under different conditions for 12 days before measuring the physiological properties of alga. The results showed that higher light and/or nitrogen levels enhanced the relative algal growth rate. The maximum net photosynthesis rate of alga increased with the light, while it remained unaffected by the nitrogen. The HN treatment had no effect on the apparent photosynthetic efficiency of algae in the LL culture, while increased it in the ML and HL cultures. The irradiance saturation point of photosynthesis was approximately 300 μmol photons m–2 s–1 with no significant difference among the six treatments, except for a slight increase under HLHN in contrast to the LLHN and MLLN treatments. HL treatment decreased the maximum quantum yield of photosynthesis (Fv/Fm) in both nitrogen levels. In the HN culture, ML and HL led to lower values of photoinhibition, indicating higher survivability in the alga. The HN culture led to higher nitrogen uptake but had no effects on Fv/Fm and the contents of pigments and soluble protein, regardless of culture light level. Based on these results, we speculate that drifting individuals of S. muticum would be possible to form a golden tide owing to its rapid growth rate at light level of 300 μmol photons m–2 s–1, when they encountered the sustained lower light level on the sea surface (≤300 μmol photons m–2 s–1). A high nitrogen supply caused by eutrophication of seawater might facilitate this process. Our results provide an important reference for the prediction of golden tides formed by S. muticum.