Assessing genetic diversity of wild populations of Prenantȁ9s schizothoracin, Schizothorax prenanti, using AFLP markers

Pawpaw (Asimina triloba) produces the largest fruit native to the United States. Six linkage groups were identified for A. triloba using the interspecific cross [PPF1-5 (A. triloba) × RET (A. reticulata Shuttlw. ex Chapman)], covering 206 centimorgans (cM). A total of 134 dominant amplification fragment length polymorphism (AFLP) markers (37 polymorphic and 97 monomorphic) were employed for estimating the genetic diversity of eight wild populations and 31 cultivars and advanced selections. For the wild populations, the percentage of polymorphic loci over all populations was 28.1% for dominant markers and Nei's genetic diversity (He) were 0.077 estimated by 134 dominant markers. Genetic diversity and the percentage of polymorphic loci estimated using only polymorphic dominant AFLPs were 0.245 and 79%, respectively, which are comparable with other plant species having the same characteristics. Estimated genetic diversity within populations accounted for 81.3% of the total genetic diversity. For cultivars and advanced selections, genetic diversity estimated by 134 dominant markers was similar to that of wild pawpaw populations (He = 0.071). Thirty-one cultivars and advanced selections were delineated by as few as nine polymorphic AFLP dominant loci. Genetic relationships among wild populations, cultivars and advanced selections were further examined by unweighted pair group method with arithmetic mean (UPGMA) of Nei's unbiased genetic distance. The genetic diversity estimated for wild populations using the clustered polymorphic markers was lower than the result estimated using the nonclustered polymorphic markers. Therefore, this study indicates that the number of sampled genomic regions, instead of the number of markers, plays an important role for the genetic diversity estimates.

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Genetic Diversity of 118 Sugarcane Germplasm Using AFLP Markers

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2014.01877 ◽

2014 ◽

Vol 40 (10) ◽

pp. 1877

Author(s):

Feng-Gang ZAN ◽

Cai-Wen WU ◽

Xue-Kuan CHEN ◽

Pei-Fang ZHAO ◽

Jun ZHAO ◽

...

Keyword(s):

Genetic Diversity ◽

Aflp Markers ◽

Sugarcane Germplasm

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Genetic diversity among five wild populations of Portunus trituberculatus

Journal of Fishery Sciences of China ◽

10.3724/sp.j.1118.2011.01327 ◽

2013 ◽

Vol 18 (6) ◽

pp. 1327-1334

Author(s):

Xiaoping LI ◽

Ping LIU ◽

Jian LI ◽

Baoquan GAO

Keyword(s):

Genetic Diversity ◽

Portunus Trituberculatus ◽

Wild Populations

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Microsatellite analysis of genetic diversity in 3 wild populations of Exopalaemon carinicauda

JOURNAL OF FISHERIES OF CHINA ◽

10.3724/sp.j.1231.2012.27900 ◽

2012 ◽

Vol 36 (12) ◽

pp. 1819

Author(s):

Shuwen JIA ◽

Ping LIU ◽

Jian LI ◽

Jitao LI ◽

Baoquan GAO ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Analysis ◽

Exopalaemon Carinicauda ◽

Wild Populations

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Microsatellite analysis on genetic diversity of seven wild populations of Scylla paramamosain in China

JOURNAL OF FISHERIES OF CHINA ◽

10.3724/sp.j.1231.2011.17102 ◽

2012 ◽

Vol 35 (7) ◽

pp. 977-984 ◽

Cited By ~ 2

Author(s):

Miao-an SHU ◽

Yu-fang ZHOU ◽

Xiao-yu ZHU ◽

Xiao-feng ZHAO ◽

Xiao-ling GUO

Keyword(s):

Genetic Diversity ◽

Microsatellite Analysis ◽

Scylla Paramamosain ◽

Wild Populations

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Mitochondrial Genetic Diversity among Farmed Stocks of Oreochromis spp. (Perciformes, Cichlidae) in Madagascar

Diversity ◽

10.3390/d13070281 ◽

2021 ◽

Vol 13 (7) ◽

pp. 281

Author(s):

Nicolas Hubert ◽

Elodie Pepey ◽

Jean-Michel Mortillaro ◽

Dirk Steinke ◽

Diana Edithe Andria-Mananjara ◽

...

Keyword(s):

Genetic Diversity ◽

Low Income ◽

Food System ◽

Production Systems ◽

Dna Barcode ◽

Diversity Indices ◽

Low Income Countries ◽

Wild Populations ◽

Fast Development ◽

Well Differentiated

The fast development of aquaculture over the past decades has made it the main source of fish protein and led to its integration into the global food system. Mostly originating from inland production systems, aquaculture has emerged as strategy to decrease malnutrition in low-income countries. The Nile tilapia (Oreochromis niloticus) was introduced to Madagascar in the 1950s, and is now produced nationally at various scales. Aquaculture mostly relies on fry harvested from wild populations and grow-out in ponds for decades. It has recently been diversified by the introduction of several fast-growing strains. Little is known how local genetic diversity compares to recently introduced strains, although high and comparable levels of genetic diversity have previously been observed for both wild populations and local stocks. Our study compares DNA barcode genetic diversity among eight farms and several strains belonging to three species sampled. DNA-based lineage delimitation methods were applied and resulted in the detection of six well differentiated and highly divergent lineages. A comparison of DNA barcode records to sequences on the Barcode of Life Data System (BOLD) helped to trace the origin of several of them. Both haplotype and nucleotide diversity indices highlight high levels of mitochondrial genetic diversity, with several local strains displaying higher diversity than recently introduced strains. This allows for multiple options to maintain high levels of genetic diversity in broodstock and provides more options for selective breeding programs.

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